Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 1025799..1026441 Replicon chromosome
Accession NZ_CP044978
Organism Bacillus thuringiensis strain BT62

Toxin (Protein)


Gene name pemK Uniprot ID J8CWW5
Locus tag DDE73_RS05495 Protein ID WP_000635963.1
Coordinates 1026091..1026441 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID R8I8H2
Locus tag DDE73_RS05490 Protein ID WP_000004570.1
Coordinates 1025799..1026086 (+) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DDE73_RS05465 1021116..1022078 + 963 WP_103621421.1 UV DNA damage repair endonuclease UvsE -
DDE73_RS05470 1022071..1022643 - 573 WP_000908523.1 rhomboid family intramembrane serine protease -
DDE73_RS05475 1022737..1023096 + 360 WP_017673683.1 holo-ACP synthase -
DDE73_RS05480 1023253..1024203 + 951 WP_002004297.1 outer membrane lipoprotein carrier protein LolA -
DDE73_RS05485 1024321..1025490 + 1170 WP_000390615.1 alanine racemase -
DDE73_RS05490 1025799..1026086 + 288 WP_000004570.1 antitoxin EndoAI Antitoxin
DDE73_RS05495 1026091..1026441 + 351 WP_000635963.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
DDE73_RS05500 1026509..1028677 + 2169 WP_142390960.1 RNA-binding transcriptional accessory protein -
DDE73_RS05505 1028735..1028851 - 117 WP_001143642.1 cortex morphogenetic protein CmpA -
DDE73_RS05510 1029047..1029505 + 459 WP_103621423.1 SprT family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12974.12 Da        Isoelectric Point: 5.7168

>T141081 WP_000635963.1 NZ_CP044978:1026091-1026441 [Bacillus thuringiensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDEVMMIRVDEALQISLGLIDF

Download         Length: 351 bp

>T141081 NZ_CP059755:183540-183806 [Enterococcus faecium]
ATGAGTAACTATACAGTCGCAATCAAAAACTCTGCGAAAGTGGATTTAAGAAAAATCAAGCAAACGAATTTAAAGAAACA
ATTTGAAGAAGTGATTCAGACATTGAAGGAAGATCCATATATGCCCACGCAGTCTTTTGAAAAGCTGAGACCTACACATG
AAGGCAGATACTCACGCAGGCTGAATAGACAGCATCGAGTGGTTTACAAAGTTGATGAGGAAAATAAAGTTGTAGAAATT
TATTCTGCCTGGACACATTATGAGTGA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10780.36 Da        Isoelectric Point: 8.9605

>AT141081 WP_000004570.1 NZ_CP044978:1025799-1026086 [Bacillus thuringiensis]
VSESSVTTEIVVRLPKQMVTELDGIGKQENKNRHELICQATQLLLRQHKTKKRYQHESMRRGYIEMGKINLGIASEAFLA
EYEAAHTVERLVSGG

Download         Length: 288 bp

>AT141081 NZ_CP059755:183277-183540 [Enterococcus faecium]
ATGGCTAATACAACGATGAATCCTAGCACTGCTCGTAAAAATTTTTATCAGTTATTGAAAGAGGTAAATGAAAATCATAC
TGAAATCGAGATTATTAGTGATCGTAGTGGAAACAATGCTGTATTGATTGGGCTGGAAGACTGGAGAGCGATTCAAGAAA
CCCTTTTTCTTGAGCAAACAGGTACTTTAGATAAAGTACGAGACCGCGAAAAAGACGACAGTAGCTTTACGAATATCGAT
GATATTGATTGGGAAGCTCTTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4HKE
PDB 7BXY

Antitoxin

Source ID Structure
AlphaFold DB A0A366FY90

References