Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relE-dinJ/YafQ-RelB
Location 75661..76192 Replicon plasmid pPBL-H3_B4-2
Accession NZ_CP044974
Organism Hydrogenophaga sp. PBL-H3 substr. PBL-H3(B4)

Toxin (Protein)


Gene name relE Uniprot ID I2ALI9
Locus tag F9Z44_RS22555 Protein ID WP_011114071.1
Coordinates 75911..76192 (+) Length 94 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID I2ALI8
Locus tag F9Z44_RS22550 Protein ID WP_011114070.1
Coordinates 75661..75924 (+) Length 88 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
F9Z44_RS22505 70867..71631 - 765 WP_011114061.1 ParA family protein -
F9Z44_RS22510 71628..71936 - 309 WP_011114062.1 KorA protein -
F9Z44_RS22515 72043..72372 - 330 WP_011114064.1 DUF2761 domain-containing protein -
F9Z44_RS22520 72374..72700 - 327 WP_011114065.1 hypothetical protein -
F9Z44_RS22525 72909..73145 - 237 WP_011114066.1 stable inheritance protein KleA -
F9Z44_RS22530 73303..73560 - 258 WP_011114067.1 hypothetical protein -
F9Z44_RS22535 73577..74785 - 1209 WP_011114068.1 hypothetical protein -
F9Z44_RS22540 74797..75060 - 264 WP_011296181.1 hypothetical protein -
F9Z44_RS22545 75060..75488 - 429 WP_011114069.1 antirestriction protein -
F9Z44_RS22550 75661..75924 + 264 WP_011114070.1 type II toxin-antitoxin system RelB/DinJ family antitoxin Antitoxin
F9Z44_RS22555 75911..76192 + 282 WP_011114071.1 type II toxin-antitoxin system YafQ family toxin Toxin
F9Z44_RS22560 76908..79823 + 2916 WP_003158660.1 Tn3-like element IS1071 family transposase -
F9Z44_RS22565 79940..80176 - 237 WP_159597444.1 hypothetical protein -
F9Z44_RS22570 80190..80846 - 657 WP_159609100.1 OmpA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - virB11 / virB4 1..104808 104808
- flank IS/Tn - - 77187..79823 2636


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-83)


Sequences

Toxin        


Download         Length: 94 a.a.        Molecular weight: 10743.19 Da        Isoelectric Point: 7.4750

>T141077 WP_011114071.1 NZ_CP044974:75911-76192 [Hydrogenophaga sp. PBL-H3]
MRQIERTGQFKRDYKREAKGQHRATLDADLVPVLVALADDQPLEPRHRDHALTGDWKDHRDCHVKPDLVLIYQKPDADTL
RLVRLGSHSELGL

Download         Length: 282 bp

>T141077 NZ_CP059748:18472-18729 [Enterococcus faecium]
ATGATTAAGGCTTGGTCTGATGATGCTTGGGATGATTATCTTTATTGGCATGAGCAAGGAAACAAAAGCAATATAAAAAA
GATTAACAAGTTAATAAAAGATATCGATCGTTCCCCCTTTGCTGGATTAGGAAAACCTGAGCCATTAAAGCATGATTTAT
CTGGAAAATGGTCCAGAAGAATTACAGATGAACATAGACTGATATATAGAGTTGAAAATGAAACGATATTTATTTATTCT
GCAAAAGATCACTATTAA

Antitoxin


Download         Length: 88 a.a.        Molecular weight: 9255.82 Da        Isoelectric Point: 5.7905

>AT141077 WP_011114070.1 NZ_CP044974:75661-75924 [Hydrogenophaga sp. PBL-H3]
MAANQLVQTRIDGAIKEEAAAVLAAMGLTVSDAVRLLLTKVAQDKALPFEPLIPNATTIEAMKEARKGKLPRFATVNDLM
ADLHAAD

Download         Length: 264 bp

>AT141077 NZ_CP059748:18210-18479 [Enterococcus faecium]
ATGGAAGCAGTAGCTTATTCAAATTTCCGCCAAAATTTACGTAGTTATATGAAACAAGTTAATGAGGATGCTGAAACACT
TATTGTAACAAGTAAAGATGTAGAAGATACAGTTGTTGTATTATCAAAAAGAGATTATGATTCTATGCAAGAAACGTTGA
GAACACTTTCTAATAATTACGTCATGGAAAAAATTCGTCGAGGAGATGAACAATTCTCCAAAGGTGCATTTAAAACACAT
GACTTAATCGAGGTTGAATCTGATGATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1U9V4I5

Antitoxin

Source ID Structure
AlphaFold DB A0A1U9V3I2

References