Detailed information of TA system TA141072

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 48441..48966
• Replicon: plasmid PNCS007098 strain 14[5]12:i:-
• Accession: NZ_CP044969
• Organism: Salmonella enterica subsp. enterica serovar 14[5]12:i:- strain PNCS007098

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: I3W3D5
• Locus tag: E5O06_RS25025
• Protein ID: WP_001159863.1
• Coordinates: 48661..48966 (+)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: M7S5D0
• Locus tag: E5O06_RS25020
• Protein ID: WP_000813641.1
• Coordinates: 48441..48659 (+)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E5O06_RS24990	43728..44109	+	382	Protein_49	YgiW/YdeI family stress tolerance OB fold protein	-
E5O06_RS24995	44278..44722	+	445	Protein_50	site-specific integrase	-
E5O06_RS25000	44981..45970	-	990	WP_001527061.1	RepB family plasmid replication initiator protein	-
E5O06_RS25005	46464..46760	-	297	WP_001687482.1	hypothetical protein	-
E5O06_RS25015	47244..47765	-	522	WP_010999942.1	hypothetical protein	-
E5O06_RS25020	48441..48659	+	219	WP_000813641.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
E5O06_RS25025	48661..48966	+	306	WP_001159863.1	type II toxin-antitoxin system toxin CcdB	Toxin
E5O06_RS25030	48968..49258	+	291	WP_001266176.1	hypothetical protein	-
E5O06_RS25035	49255..49776	+	522	WP_000198608.1	hypothetical protein	-
E5O06_RS25040	49811..50593	+	783	WP_000082169.1	site-specific integrase	-
E5O06_RS25045	50602..51315	+	714	Protein_59	EAL domain-containing protein	-
E5O06_RS25055	51319..51828	+	510	WP_001541544.1	CaiF/GrlA family transcriptional regulator	-
E5O06_RS25060	51822..52307	+	486	WP_000905606.1	membrane protein	-
E5O06_RS25065	52584..52871	-	288	WP_071530243.1	hypothetical protein	-
E5O06_RS25070	53027..53587	+	561	WP_000900095.1	inverse autotransporter beta domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..93724	93724

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (25-87)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (5-69)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 13091.77 Da
• Isoelectric Point: 12.8043

>T141072 WP_001159863.1 NZ_CP044969:48661-48966 [Salmonella enterica subsp. enterica serovar 1,4,[5],12:i:-]
KKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKK
KKKKKKKKKKKKKKKKKKKKKK

Nucleotide

• Length: 306 bp

>T141072 NZ_CP059740:c216679-216407 [Enterococcus faecium]
ATGAATAACTATTCTGTGATGATTAAAAACTCGGCTAAAGCTGATTTAAAGAAAATCAAACAGTCTAATTTGAAACCTCA
ATTTGAGAAGGTTATTCAGACTTTAAAGGAAGATCCCTATCTCCCTACCCAATCATTTGAGAAGTTAAAACCTACACATG
AAGGTAGGTATTCTAGAAGATTGAATAGACAACACCGGGTTGTTTATAAGGTAGATGAAGATGAACACGTGGTTGAAATT
TATTCAGCAAGGACGCATTACGAGTCACAGTAA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 9374.72 Da
• Isoelectric Point: 12.6573

>AT141072 WP_000813641.1 NZ_CP044969:48441-48659 [Salmonella enterica subsp. enterica serovar 1,4,[5],12:i:-]
KKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKK

Nucleotide

• Length: 219 bp

>AT141072 NZ_CP059740:c216942-216679 [Enterococcus faecium]
ATGGCAAAAACAGTCTTAAATCCTAGCTCAGCACGGAAAAATTTTTATAAGTTGTTGAAAGATGTAAATGAGAATCATAC
GGAAATTGAAATCATTAGTGAGCGCAGTGAAAATAATGCGGTGCTTATTGGTTTGGATGATTGGAAAGCAATCAAAGAAA
CGCTATTACTTGAGCAAATAGGCACTTTGGGTGTTGTTCGTAAGCGAGAAAAAGATGATAGTGGATTTACTAATGTTGAT
GAAATTGATTGGGATAACTTATAA

Structures

Toxin

• AlphaFold DB: I3W3D5

Antitoxin

• AlphaFold DB: A0A656ICA6