Detailed information of TA system TA141061

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /TIGR02293(antitoxin)
• Location: 3251597..3252479
• Replicon: chromosome
• Accession: NZ_CP044546
• Organism: Pseudomonas sp. CFA

Toxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: F7661_RS14495
• Protein ID: WP_190870159.1
• Coordinates: 3252132..3252479 (+)
• Length: 116 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: I7C3Q7
• Locus tag: F7661_RS14490
• Protein ID: WP_003250525.1
• Coordinates: 3251597..3252046 (+)
• Length: 150 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F7661_RS14465	3247020..3248219	+	1200	WP_104962081.1	sugar transporter	-
F7661_RS14470	3248259..3248843	-	585	WP_104962080.1	LysE family transporter	-
F7661_RS14475	3248895..3249725	-	831	WP_003250518.1	AraC family transcriptional regulator	-
F7661_RS14480	3249774..3250697	-	924	WP_104962079.1	LysR family transcriptional regulator	-
F7661_RS14485	3250801..3251454	+	654	WP_014591578.1	oxygen-insensitive NAD(P)H-dependent nitroreductase NfsB	-
F7661_RS14490	3251597..3252046	+	450	WP_003250525.1	DUF2384 domain-containing protein	Antitoxin
F7661_RS14495	3252132..3252479	+	348	WP_190870159.1	RES family NAD+ phosphorylase	Toxin
F7661_RS14500	3252537..3253691	-	1155	WP_129934025.1	aminotransferase class V-fold PLP-dependent enzyme	-
F7661_RS14505	3253886..3254812	-	927	WP_104962078.1	LysR family transcriptional regulator	-
F7661_RS14510	3254953..3256182	+	1230	WP_020192828.1	acyl-CoA dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (1-108)

Antitoxin

• Antitoxin Xre-like, helix-turn-helix domain (33-91)

Sequences

Toxin

Protein

• Length: 116 a.a.
• Molecular weight: 12727.57 Da
• Isoelectric Point: 4.3696

>T141061 WP_190870159.1 NZ_CP044546:3252132-3252479 [Pseudomonas sp. CFA]
VVYAATSPPGAMLEVLVHLEIDPEDFPTTMRLLRIELPDTVSQAQLPALQPGWSAQPELTRTLGNRFLDDCSALLLPVPS
AIMPSTTNYLFNPRHPQAQSAKIQVEDFTPDSRLF

Nucleotide

• Length: 348 bp

>T141061 NZ_CP059735:c2420335-2420033 [Thalassomonas actiniarum]
ATGACCCCCTTCGTTTTAACCAACGCTGCGAACTCTGATTTGAAAAAAATCGCCAGGTCTACCCAAGGGAAATGGGGAAA
TGAGCAAAGAAACTTTTACATTAAACAATTTGACGATGCATCCCATATGCTTGCCAAGACCCCTCTCGCCGGTAAATCTT
GTGACGAAACTCGAGCTGGCTACAAAAAGTTTCCTGTTGGTAGCCACTTAATTTTTTATATAAAAGGCGAAAATTCAAGA
ATAAAAATTGTTCGCATCCTGCATAAAAGAACGGACGTCGAACTAAACCTTCAGGCGCCATAA

Antitoxin

Protein

• Length: 150 a.a.
• Molecular weight: 16956.46 Da
• Isoelectric Point: 5.7258

>AT141061 WP_003250525.1 NZ_CP044546:3251597-3252046 [Pseudomonas sp. CFA]
MLAEVLRDNGYHEYRARLQALLDIPELASDFEIHTRITDGFAATWLVKLTERGVLTPVERDQIIPLRTLKSRIERDQPLT
VDESDRLFRSAHITAMAEAVFGEAGKAKRWLSKPKERFSGLTPMQMLTTQQGTTQVEEMLLQIAEGYGL

Nucleotide

• Length: 450 bp

>AT141061 NZ_CP059735:c2420574-2420332 [Thalassomonas actiniarum]
ATGGCCAAAAACACTAGCATCACCCTTGGAGATCATTTTGACAGCTTTATCGCCAGCCAAATAGGTAGCGGGCGTTATGG
CTCTGCCAGCGAGGTTATTCGCGCAGGATTGAGATTACTTGAAAACACCGAGACAAAAACAGAGACTTTACGCCGTATGC
TTGTAGAGGGTGAGAAAAGCGGCACTGCAGATTACACCTTTGATACATTTATCAATGAACTTGATGGTGAAATTGAGAAA
TGA

Structures

Antitoxin

• AlphaFold DB: I7C3Q7