Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazF-pemI/MazF(toxin)
Location 228274..228910 Replicon chromosome
Accession NZ_CP044545
Organism Pradoshia sp. D12

Toxin (Protein)


Gene name mazF Uniprot ID A0A1B9AZ20
Locus tag F7984_RS01270 Protein ID WP_066102464.1
Coordinates 228548..228910 (+) Length 121 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID A0A0K9GID8
Locus tag F7984_RS01265 Protein ID WP_049672447.1
Coordinates 228274..228555 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
F7984_RS01245 224480..225067 - 588 WP_066102476.1 rhomboid family intramembrane serine protease -
F7984_RS01250 225164..225517 + 354 WP_066102473.1 holo-ACP synthase -
F7984_RS01255 225822..226817 + 996 WP_066102470.1 outer membrane lipoprotein carrier protein LolA -
F7984_RS01260 226941..228113 + 1173 WP_066102467.1 alanine racemase -
F7984_RS01265 228274..228555 + 282 WP_049672447.1 hypothetical protein Antitoxin
F7984_RS01270 228548..228910 + 363 WP_066102464.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
F7984_RS01275 229249..230085 + 837 WP_066102461.1 STAS domain-containing protein -
F7984_RS01280 230082..230438 + 357 WP_066102458.1 STAS domain-containing protein -
F7984_RS01285 230443..230844 + 402 WP_066102671.1 anti-sigma regulatory factor -
F7984_RS01290 230856..231866 + 1011 WP_139063803.1 PP2C family protein-serine/threonine phosphatase -
F7984_RS01295 231929..232261 + 333 WP_066102453.1 anti-sigma factor antagonist -
F7984_RS01300 232258..232731 + 474 WP_066102448.1 anti-sigma B factor RsbW -
F7984_RS01305 232709..233509 + 801 WP_066102445.1 RNA polymerase sigma factor SigB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(8-115)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 121 a.a.        Molecular weight: 13342.41 Da        Isoelectric Point: 5.8683

>T141060 WP_066102464.1 NZ_CP044545:228548-228910 [Pradoshia sp. D12]
VNKGLNVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIIAAITAQIQKAKLPTHVEIDAQKYGFERDSVIL
LEQIRTIDKQRLTDKITQLDDEMMRKVDNALQISLGLVEL

Download         Length: 363 bp

>T141060 NZ_CP059735:c2391911-2391606 [Thalassomonas actiniarum]
ATGGCAAAATACCGGGTCACACCCCAAGCCCGTGATGCTCTAAAAAATATAGCTCGATATACGGAACAACAATGGGGGAA
AAACCAACGAAACATTTACCTTAAGAACCTGGAAGCAGCCTTTAATACTCTTGCGGAAAACCCAAAGCTTGGCAAGCATA
GAGCAGATATCCATGAAGGTTATTACAGCTTCGCACAAGGGCAGCATGTTATCTTTTACCTTATCCATCGCCAAGGCATA
GATATCATAGGCATTCCCCATAAAAAAATGGACATTATCCATTATTTTCATTCCAGTAATTATTAA

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10870.23 Da        Isoelectric Point: 4.6121

>AT141060 WP_049672447.1 NZ_CP044545:228274-228555 [Pradoshia sp. D12]
VSESSATTEILIRLPQNLLSELDGYVREENVNRSEFIYQATKMYLQERKKKEFRESMRRGYMEMANINLTMAAEAFQAEY
EAEHAVERLVSGE

Download         Length: 282 bp

>AT141060 NZ_CP059735:c2392159-2391911 [Thalassomonas actiniarum]
ATGGCCACGACAAGTCTCAGCCTGGGCGAGCATTGGGAAGCCTTCATTAAAGATGAAATAGCCAGTGGCCGTTACGGCTC
CGCCAGTGAAGTTGTTCGCGATGCATTGCGCACTATGGAAGAGCGTAAAACTAAATTGGCCGCCCTAAGAGCACATTTGG
CAACAGGGGCTCAACAAGCTGCCTCAGGGGACTTCGTTGATGACTTTTCTATGGATTCATTAATTGCCGATTTAGATAAT
AACGAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1B9AZ20

Antitoxin

Source ID Structure
AlphaFold DB A0A0K9GID8

References