Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 5792..6317 Replicon plasmid plamid_2
Accession NZ_CP044529
Organism Klebsiella grimontii strain SS141

Toxin (Protein)


Gene name ccdB Uniprot ID D4HQE7
Locus tag EHW69_RS29650 Protein ID WP_013023785.1
Coordinates 5792..6097 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID W8V2V6
Locus tag EHW69_RS29655 Protein ID WP_001568025.1
Coordinates 6099..6317 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EHW69_RS29620 1527..2153 + 627 WP_004197649.1 ParA family plasmid-partitioning AAA ATPase -
EHW69_RS29625 2273..2452 + 180 WP_006788217.1 hypothetical protein -
EHW69_RS29630 2868..3662 - 795 WP_004197635.1 site-specific integrase -
EHW69_RS29635 3860..4876 - 1017 WP_017899884.1 hypothetical protein -
EHW69_RS29640 4887..5201 - 315 WP_053389906.1 hypothetical protein -
EHW69_RS29645 5228..5623 - 396 WP_017899885.1 hypothetical protein -
EHW69_RS29650 5792..6097 - 306 WP_013023785.1 type II toxin-antitoxin system toxin CcdB Toxin
EHW69_RS29655 6099..6317 - 219 WP_001568025.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
EHW69_RS29665 6487..6732 - 246 Protein_9 hypothetical protein -
EHW69_RS29670 6872..7561 + 690 WP_004193995.1 hypothetical protein -
EHW69_RS29675 7593..8282 - 690 WP_020315560.1 RES family NAD+ phosphorylase -
EHW69_RS29680 8719..8967 + 249 WP_000272716.1 hypothetical protein -
EHW69_RS29685 8964..9536 + 573 WP_020805590.1 hypothetical protein -
EHW69_RS29690 9567..10061 + 495 WP_020805591.1 hypothetical protein -
EHW69_RS29695 10105..10473 + 369 WP_004197807.1 hypothetical protein -
EHW69_RS29700 10507..10710 + 204 WP_004197808.1 hemolysin expression modulator Hha -
EHW69_RS29705 10724..10927 + 204 WP_004197809.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..100742 100742


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11615.30 Da        Isoelectric Point: 6.4672

>T141056 WP_013023785.1 NZ_CP044529:c6097-5792 [Klebsiella grimontii]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESYRLMTTDMASVTSSVTGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T141056 NZ_CP059733:1858918-1859193 [Thalassomonas viridans]
ATGGAAATTAAATGGACGATTAAATCCCTTTCAGATTTAAATCGTCTGTTTGAATTCTTGGCGCCGGTAAATAAACAAGC
TGCGGCCAAGACCATACAATCCCTTACCCAGGCACCTACCCGAATCATTCATCAACCTCGCATTGGTGAGAGGCTTGATG
AATTTGAGCCAAAAGAAGTTCGCCGACTGTTAGTTGGGCATTATGAAATGCGTTATGAATTGAGGAATAATACCTTATTT
ATACTTCGAATTTGGCATACCCGGGAAAGAAGGTAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8392.34 Da        Isoelectric Point: 4.7405

>AT141056 WP_001568025.1 NZ_CP044529:c6317-6099 [Klebsiella grimontii]
MKQRITVTIDSDSYQLLKSADVNISALVNRAMQNEARRLRSERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT141056 NZ_CP059733:1858655-1858918 [Thalassomonas viridans]
ATGATGGAAACTAAAGTTATAACAGCCCATGTTCCGATATCATTAGCTGAAAAGGTTGATAATTTAGCTAATCGTTTAGA
GCGCTCAAAAGCCTGGGTAATGAAACAAGCATTGGCTGCATGGGTTGAGCAGGAAGAAGAGCGTCACCGTTTGACACTCG
AAGCGATAGCAGATGTTGATGAAAATAATGTTATAGATCATCAGTCAGTGCAGGCATGGGCTGAAAGTTTAAGTTCAGAT
ACTCCTTTACCGACTCCCCGTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2J4ZZW6

Antitoxin

Source ID Structure
AlphaFold DB A0A2J4ZZP8

References