Detailed information of TA system TA141055

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 68187..68923
• Replicon: plasmid plamid_1
• Accession: NZ_CP044528
• Organism: Klebsiella grimontii strain SS141

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: A0A2J5Q928
• Locus tag: EHW69_RS29280
• Protein ID: WP_004098919.1
• Coordinates: 68441..68923 (+)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: A0A2J5Q9C0
• Locus tag: EHW69_RS29275
• Protein ID: WP_004098921.1
• Coordinates: 68187..68453 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EHW69_RS29230	63444..64022	-	579	WP_009654302.1	hypothetical protein	-
EHW69_RS29235	64312..64575	+	264	WP_009310051.1	hypothetical protein	-
EHW69_RS29240	64572..65138	+	567	WP_009654312.1	hypothetical protein	-
EHW69_RS29245	65169..65663	+	495	WP_013023776.1	hypothetical protein	-
EHW69_RS29250	65707..66075	+	369	WP_063923539.1	hypothetical protein	-
EHW69_RS29255	66106..66309	+	204	WP_004098931.1	hemolysin expression modulator Hha	-
EHW69_RS29260	66358..66615	+	258	WP_004098928.1	hypothetical protein	-
EHW69_RS29265	66844..67812	-	969	WP_163467572.1	IS5 family transposase	-
EHW69_RS29275	68187..68453	+	267	WP_004098921.1	DUF1778 domain-containing protein	Antitoxin
EHW69_RS29280	68441..68923	+	483	WP_004098919.1	GNAT family N-acetyltransferase	Toxin
EHW69_RS29285	69132..70478	+	1347	WP_077255298.1	ISNCY family transposase	-
EHW69_RS29290	70537..71328	+	792	WP_003032641.1	tetratricopeptide repeat protein	-
EHW69_RS29295	71369..72151	-	783	Protein_85	hypothetical protein	-
EHW69_RS29300	72141..72491	+	351	Protein_86	transposase	-
EHW69_RS29305	72522..72860	+	339	Protein_87	IS5/IS1182 family transposase	-
EHW69_RS29310	72906..73097	-	192	WP_127074617.1	hypothetical protein	-
EHW69_RS30555	73094..73255	-	162	WP_003032086.1	hypothetical protein	-
EHW69_RS29315	73327..73778	-	452	Protein_90	transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	mrkJ / mrkF / mrkD / mrkC / mrkB / mrkA	1..133593	133593
	-	inside	IScluster/Tn	-	-	66844..72860	6016

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (66-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17335.06 Da
• Isoelectric Point: 10.0704

>T141055 WP_004098919.1 NZ_CP044528:68441-68923 [Klebsiella grimontii]
VGRITAPEPLSSAHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKKGTKKVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Nucleotide

• Length: 483 bp

>T141055 NZ_CP059733:c1618598-1618326 [Thalassomonas viridans]
ATGCTCGCCCTTGAGTATGCCTCGCAATTTAAAAAAGACTTTAAAAAAGTTGCAAAACTACCAATACCTGATGTTATTGA
AGTTGGCCACGTTATAAAGCAATTGCAATTAGGGCAAACTTTACCAAAAAAGTATGTAGATCATTCTTTATCTGGAAACT
GGCAAGGTTTTCGAGATTGCCATATTAAACCTGACTTGGTTTTGATATATAAAATTGATAACGTTTCTTTAAAACTTGCC
CGAATTGGAACGCATAGCGAGCTTTTTCGCTAG

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10105.49 Da
• Isoelectric Point: 4.5141

>AT141055 WP_004098921.1 NZ_CP044528:68187-68453 [Klebsiella grimontii]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEIACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDDSAINKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT141055 NZ_CP059733:c1618870-1618598 [Thalassomonas viridans]
ATGAAAACAGAAATGCTAAGTACACGCATAGACCCTGATACAAAGTTAGCGTTTACCCGCGTTTGCGAAGACATGGGGCT
AAGCACCTCACAAGCTTTAAAGCTATTTGCAAAAGCTGTGATCAATCATGGCGGAATACCTTTTGAACTGAAAGCGAAAA
CCCCTAATGCATTAACAGCGGCTGCGATAAATGAACTGGAAACCGGAAATGGCAAAACAGCCAATACGGTTGAAGAGTTA
TTTAATGATTTAGATATTGATAGCCTTAAATAA

Structures

Toxin

• AlphaFold DB: A0A2J5Q928

Antitoxin

• AlphaFold DB: A0A2J5Q9C0