Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF-MazE
Location 4813..5378 Replicon plasmid p1
Accession NZ_CP044515
Organism Leptospira interrogans serovar Canicola strain 611

Toxin (Protein)


Gene name mazE Uniprot ID A0A0C5XAD8
Locus tag F8B19_RS20245 Protein ID WP_000237066.1
Coordinates 4813..5136 (-) Length 108 a.a.

Antitoxin (Protein)


Gene name mazF Uniprot ID A0A0F6H5X2
Locus tag F8B19_RS20250 Protein ID WP_000648189.1
Coordinates 5130..5378 (-) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
F8B19_RS20205 1..1148 + 1148 WP_151419827.1 IS3-like element IS1500B family transposase -
F8B19_RS20210 1212..2012 - 801 WP_057141741.1 IS3 family transposase -
F8B19_RS20215 2040..2339 - 300 WP_000678552.1 transposase -
F8B19_RS20225 2990..3331 - 342 WP_000240418.1 endoribonuclease MazF -
F8B19_RS20230 3325..3561 - 237 WP_000443825.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
F8B19_RS20235 3906..4328 - 423 WP_000402980.1 hypothetical protein -
F8B19_RS20240 4377..4589 - 213 WP_000447737.1 hypothetical protein -
F8B19_RS20245 4813..5136 - 324 WP_000237066.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
F8B19_RS20250 5130..5378 - 249 WP_000648189.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
F8B19_RS20255 6165..6379 + 215 Protein_9 hypothetical protein -
F8B19_RS20260 6604..7383 + 780 Protein_10 leucine-rich repeat domain-containing protein -
F8B19_RS20265 7655..8593 + 939 WP_000852605.1 Rpn family recombination-promoting nuclease/putative transposase -
F8B19_RS20270 9159..9314 - 156 WP_001976969.1 type II toxin-antitoxin system RelB/DinJ family antitoxin -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..75928 75928


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(2-105)

Antitoxin

SpoVT-AbrB domain

(6-49)


Sequences

Toxin        


Download         Length: 108 a.a.        Molecular weight: 12168.59 Da        Isoelectric Point: 10.5161

>T141032 WP_000237066.1 NZ_CP044515:c5136-4813 [Leptospira interrogans serovar Canicola]
MVISQYEVYLINLDPTVGHEIKKSRPCAIISPNEMNKTIGTIIIAPMTTKSRSYPTRVELTFQGKKGWIVLDQIRAVDKT
RLVKKLGKIDPKTVNKMKLVIKEMLVD

Download         Length: 324 bp

>T141032 NZ_CP059723:c362413-362306 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9507.04 Da        Isoelectric Point: 5.1749

>AT141032 WP_000648189.1 NZ_CP044515:c5378-5130 [Leptospira interrogans serovar Canicola]
MKASVVKIGNSKGIRIPKAVLEECHIEEEVDLLIDNNKLIIVPLKSKPREGWEKQFKTMSSNKDDKLFIPDSIDLLDKDW
EW

Download         Length: 249 bp

>AT141032 NZ_CP059723:362461-362527 [Escherichia coli]
GTTCTGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTGTACCTCTCAACGTGCGGGGGTTTTCTC

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0C5XAD8

Antitoxin

Source ID Structure
AlphaFold DB A0A0F6H5X2

References