Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF-MazE
Location 4762..5327 Replicon plasmid p1
Accession NZ_CP044511
Organism Leptospira interrogans serovar Canicola strain LJ178

Toxin (Protein)


Gene name mazE Uniprot ID A0A0C5XAD8
Locus tag F8A85_RS20235 Protein ID WP_000237066.1
Coordinates 4762..5085 (-) Length 108 a.a.

Antitoxin (Protein)


Gene name mazF Uniprot ID A0A0F6H5X2
Locus tag F8A85_RS20240 Protein ID WP_000648189.1
Coordinates 5079..5327 (-) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
F8A85_RS20195 1..1098 + 1098 Protein_0 IS3 family transposase -
F8A85_RS20200 1162..1809 - 648 WP_151419861.1 IS3 family transposase -
F8A85_RS20205 1989..2288 - 300 WP_000678552.1 transposase -
F8A85_RS20215 2939..3280 - 342 WP_000240418.1 endoribonuclease MazF -
F8A85_RS20220 3274..3510 - 237 WP_000443825.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
F8A85_RS20225 3855..4277 - 423 WP_000402980.1 hypothetical protein -
F8A85_RS20230 4326..4538 - 213 WP_000447737.1 hypothetical protein -
F8A85_RS20235 4762..5085 - 324 WP_000237066.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
F8A85_RS20240 5079..5327 - 249 WP_000648189.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
F8A85_RS20245 6114..6328 + 215 Protein_9 hypothetical protein -
F8A85_RS20250 6553..7332 + 780 WP_000905289.1 leucine-rich repeat domain-containing protein -
F8A85_RS20255 7604..8542 + 939 WP_000852605.1 Rpn family recombination-promoting nuclease/putative transposase -
F8A85_RS20260 9108..9263 - 156 WP_001976969.1 type II toxin-antitoxin system RelB/DinJ family antitoxin -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..75855 75855


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(2-105)

Antitoxin

SpoVT-AbrB domain

(6-49)


Sequences

Toxin        


Download         Length: 108 a.a.        Molecular weight: 12168.59 Da        Isoelectric Point: 10.5161

>T141028 WP_000237066.1 NZ_CP044511:c5085-4762 [Leptospira interrogans serovar Canicola]
MVISQYEVYLINLDPTVGHEIKKSRPCAIISPNEMNKTIGTIIIAPMTTKSRSYPTRVELTFQGKKGWIVLDQIRAVDKT
RLVKKLGKIDPKTVNKMKLVIKEMLVD

Download         Length: 324 bp

>T141028 NZ_CP059712:93348-94673 [Enterobacter hormaechei]
ATGAGCCGGAAACAGCAGCGTCTGGCAATCTGGATGAACGGAATCAAAGTCGGATACTGGGAAAAAAGTAAAGGGGTAGA
CAGCTTACAATACCTTCCTGACTGGAGTGCAGACGAACAGGGAAGGCCGCTGTCATTGTCCCTTCCTTTTACTCCCGGGA
ACCAGGTCTGGCGTGGAAATGTGGTACGTGACTATTTTGATAATTTACTCCCGGACAGCGAAGGCATACGCAGACGTCTG
GCAATGCGTTACAAGGCCGATAGCCTGGAGCCTTTTGATCTTCTGACGGAGCTGGGAAAAGACTGCGTGGGCGCGATACA
GCTGCTTCATGACGGAGATGAACCCACAGATTTATATTCCGTGAAGTATCACCCGCTTACTGAATCAGAAATTGCGGCAA
CTTTGCGTAATACCACGGAGACATTGCTACCGGGCAGGCCAGAAGATAACGACGACTTACGTTTATCTATTGCCGGTGCT
CAGGAGAAAACAGCCCTACTGTGGCATGAAGATCGGTGGTGTATGCCGGAGGGTAATACACCAACGACGCATATATTCAA
GCTACCGCTCGGACTTGTGGGGAACATGAAAGCCGACATGCGCTCGTCGGTTGAAAATGAATGGCTCTGCTCGGTACTTC
TCGAACAGTACGGGCTCCCCGTGGCAAGAACGCAGATTGCTCACTTTGAAGATCAGAAAGCGCTGGTGGTAGAACGTTTT
GACCGAAAATGGTCAGGAAACGGACAATGGATCATTCGTTTACCCCAGGAGGATATGTGTCAGGCCCTGGGCATTTCGCC
ATTACGAAAATATCAGGCGGACGGCGGGCCAGGTATATCGGAAATTATGGAAGTTCTGAGTAATTCAGACCGTGCTGAGC
GTGACAAAGCGCAGTTTTTTATGACGCAGATAGTTTTCTGGATGATGGCCGCAACAGATGGCCACGCTAAAAATTTCAGT
ATTTCTATTGGGCCACAGGGACGTTATCACCTTACACCACTCTATGATGTGTTGTCGGCATGGCCGGTGATCGGTCATGG
GAACAATCAGATATCCTGGCAGAAGTGCAAACTGGCGATGGCCGTACGTGGCAGTAGCAACTATTACCAGATATATCGAA
TACAACGACGCCACTGGATCAGGCATGGTGAGATAACCGGCCTGAGTAAACAGCAGACAGAGGCGATGATAGAGGAAATC
ATCGCCAGAACCCCGGGTGTCATTGAACGTGTTAGCGGGCTTCTTCCGGACCAGTTCCCCCAGCAGCTTGCGGAAAGTAT
TTTTGATGGAATGAGGCAGCAATGCAGGCGCCTGGCTGAGAAATGA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9507.04 Da        Isoelectric Point: 5.1749

>AT141028 WP_000648189.1 NZ_CP044511:c5327-5079 [Leptospira interrogans serovar Canicola]
MKASVVKIGNSKGIRIPKAVLEECHIEEEVDLLIDNNKLIIVPLKSKPREGWEKQFKTMSSNKDDKLFIPDSIDLLDKDW
EW

Download         Length: 249 bp

>AT141028 NZ_CP059712:93050-93343 [Enterobacter hormaechei]
ATCAATAATGATTACCCGTTAAACACGCTCAACCAGTTGCGTCCCTTGCTCATTGGTTTTCGCAAGGCGAATGGCCTGAC
GCAAAAAGACCTGTCCGAAAGGTTAGGGGTCACTCAACAGACATACTCACGTCTGGAAGCCAACCCTGCCAGTGCGAGCA
TTGAACGGCTATTCAAGGTGTTTTCTATCCTGGGCGTAAAAATCAGCTTCTCCTCGACAACCGCCTCTTCAGAGGGGAAG
CAGACGGAAGAAATGCTTAAATCAAACTCACCTGCACGACAGGAGAAATGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0C5XAD8

Antitoxin

Source ID Structure
AlphaFold DB A0A0F6H5X2

References