141027

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 2939..3510 Replicon plasmid p1
Accession NZ_CP044511
Organism Leptospira interrogans serovar Canicola strain LJ178

Toxin (Protein)


Gene name mazF Uniprot ID Q8F5A3
Locus tag F8A85_RS20215 Protein ID WP_000240418.1
Coordinates 2939..3280 (-) Length 114 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID M6U553
Locus tag F8A85_RS20220 Protein ID WP_000443825.1
Coordinates 3274..3510 (-) Length 79 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
F8A85_RS20195 1..1098 + 1098 Protein_0 IS3 family transposase -
F8A85_RS20200 1162..1809 - 648 WP_151419861.1 IS3 family transposase -
F8A85_RS20205 1989..2288 - 300 WP_000678552.1 transposase -
F8A85_RS20215 2939..3280 - 342 WP_000240418.1 endoribonuclease MazF Toxin
F8A85_RS20220 3274..3510 - 237 WP_000443825.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
F8A85_RS20225 3855..4277 - 423 WP_000402980.1 hypothetical protein -
F8A85_RS20230 4326..4538 - 213 WP_000447737.1 hypothetical protein -
F8A85_RS20235 4762..5085 - 324 WP_000237066.1 type II toxin-antitoxin system PemK/MazF family toxin -
F8A85_RS20240 5079..5327 - 249 WP_000648189.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
F8A85_RS20245 6114..6328 + 215 Protein_9 hypothetical protein -
F8A85_RS20250 6553..7332 + 780 WP_000905289.1 leucine-rich repeat domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..75855 75855


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(7-112)

Antitoxin

Antitoxin PemI-like

(1-78)


Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 12918.12 Da        Isoelectric Point: 9.9595

>T141027 WP_000240418.1 NZ_CP044511:c3280-2939 [Leptospira interrogans serovar Canicola]
MVKNRNYTPEKGDIVWLNFTPQAGHEQKGRRPALVLSPKEYNSKTGLAIFCPITSKIKGYPFEVLIKSKKIDGVILSDQV
KNLDWTIREAEFIESINKVSLKEVLDNIKLLIF

Download         Length: 342 bp

>T141027 NZ_CP059711:c4867750-4867379 [Enterobacter hormaechei]
ATGGAGCACATGGCTGTGTATGACACCAATATCCTCATCGATCTTTTTAATAACCACGTTGAGGCTGCGGATGCGATCGA
TCGCACAGCTTCGCATCGTGCCATTAGCCTCATCACATGGATGGAAGTAATGGTGGGCGCTCGCAGGCACGGTCAAGAAG
CAAAAACGGCAGCCGTGATGGGGGCCTTTGAAATTATTGATATAACAAGGGAAATTGCTGAAAGAAGCGTATTACTTCGC
GAACAGCACGGGATGAAGCTTCCTGACGCCATTATTCTGGCTACTGCTCAGAGCAGAAGCTGTCCGTTAATTTCGCGCAA
TACGAAAGACTTTTCGGGTATTGCTGGGGTCGTTTCACCTTACCACCTGTAG

Antitoxin


Download         Length: 79 a.a.        Molecular weight: 8728.95 Da        Isoelectric Point: 5.6663

>AT141027 WP_000443825.1 NZ_CP044511:c3510-3274 [Leptospira interrogans serovar Canicola]
MESIIQKWGNSLGIRIPKAMATELELNDGSHVELQYEGDKIVIYPMKKASLEDKLSKITKQNLHSEISTGNSIGNEAW

Download         Length: 237 bp

>AT141027 NZ_CP059711:c4867995-4867753 [Enterobacter hormaechei]
ATGAGTCTTTTGGCGGACCTTACTATGGGCAGAATACTGATCGATTTGTCTGATGACGTGATTCAACGACTGGATAACCT
GAAGCAGTTGCGCAACCGGCCGCGCGCCGAACTGCTGCGGGAAGCCATAGAACTGTATCTCGACCAGCAGAATTCATCGG
TTATCCGCGAAGCGCTTGGCCTGTGGGGAAATCAGCAGGAAGATGGGCTTGAGTACGAACGCAAGCTGCGTGAGGAGTGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4D8SL30

Antitoxin

Source ID Structure
AlphaFold DB A0A0F6HC33

References