Detailed information of TA system TA141026

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): chpIK/-
• Location: 2781821..2782392
• Replicon: chromosome
• Accession: NZ_CP044509
• Organism: Leptospira interrogans serovar Canicola strain LJ178

Toxin (Protein)

• Gene name: chpK
• Uniprot ID: -
• Locus tag: F8A85_RS12415
• Protein ID: WP_181016507.1
• Coordinates: 2781821..2782051 (-)
• Length: 77 a.a.

Antitoxin (Protein)

• Gene name: chpI
• Uniprot ID: A0A8I0U107
• Locus tag: F8A85_RS12420
• Protein ID: WP_000844756.1
• Coordinates: 2782144..2782392 (-)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F8A85_RS12390	2777122..2777526	-	405	WP_001974506.1	pyridoxamine 5'-phosphate oxidase family protein	-
F8A85_RS12395	2777523..2777990	-	468	WP_000060052.1	hypothetical protein	-
F8A85_RS12405	2778794..2779729	+	936	WP_000004530.1	ornithine carbamoyltransferase	-
F8A85_RS12410	2779869..2780861	+	993	WP_000041094.1	aldolase	-
F8A85_RS12415	2781821..2782051	-	231	WP_181016507.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
F8A85_RS12420	2782144..2782392	-	249	WP_000844756.1	ribbon-helix-helix domain-containing protein	Antitoxin
F8A85_RS12435	2784167..2784463	-	297	WP_000477061.1	helix-turn-helix domain-containing protein	-
F8A85_RS21040	2784617..2785076	+	460	Protein_2277	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (2-71)

Antitoxin

• Predicted DNA-binding protein, ribbon-helix-helix domain (2-41)

Sequences

Toxin

Protein

• Length: 77 a.a.
• Molecular weight: 8093.39 Da
• Isoelectric Point: 9.8976

>T141026 WP_181016507.1 NZ_CP044509:c2782051-2781821 [Leptospira interrogans serovar Canicola]
INTIVSIAITSNLNLSEAPGNVLISKKDSSLSKDSVINVSQIVTLDKERFLNKAGKLKSNKLGEVEIGLKLVTGLD

Nucleotide

• Length: 231 bp

>T141026 NZ_CP059711:c4198660-4198283 [Enterobacter hormaechei]
ATGCAAACCCAACCACTATCCTCAACTCAGGAGGCTACACCACGCCCGTCACCGGTGGAGATCTGGCAACGGCTTCTGAG
CCACCTGTTGGATCGTCACTACGGTCTGACACTCAACGACACTCCGTTCGGCAACGATGGCGTGATTCAGGAGCATATCG
ACGCCGGTATCTCACTGTGCGACGCGGTGAATTTTATCGTGGAGAAATACGATCTGGTACGAACCGACCGTCGCGGTTTT
AACGCAGAAACACAGTCGCCTCTTCTCAGCAGTATCGATATTCTCCGCGCCCGCAAAGCCACCGGACTGATGACCCGAAA
CGATTACAGAACGGTGACCGACATCACCACCGGTAAATACCGGGAGGTGCAGCCATGA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 9324.75 Da
• Isoelectric Point: 10.3019

>AT141026 WP_000844756.1 NZ_CP044509:c2782392-2782144 [Leptospira interrogans serovar Canicola]
MKTAISIPDELFKAAEKIAKKLGIPRSQLFAKALEEFIQSHSKESVTEKLNKIYSNKSKETKNNITDLSVESLRKSLKND
SW

Nucleotide

• Length: 249 bp

>AT141026 NZ_CP059711:c4199037-4198711 [Enterobacter hormaechei]
ATCACTGAACCCTGGTGGGGGCTTAAACGCAGTATCACGCCCTGTTTTGGCGCACGGCTGGTACAGGCGGGCAACCGTCT
GCATTATCTCGCTGACCGGGCCAACATCACCGGACAGTTCAGCGACGCTGATTTACGGTATCTCGACCAGGCATTTCCAC
TGCTGCTCAAACAGCTTGAACTGATGCTCACCAGCGGTGAACTCAATCCCTGCCATCAGCACTGTGTCACGCTCTATGCA
AAAGGGCTGACCTGCGAGGCCGACTCCCTCGGCTCGCATGGTTACGTATACCTGGCGATTTATCCGACACCAGCGGCAAC
CGCATAA