141023

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) spoIISABC/SpoIISA-SpoIISB
Location 1304685..1305601 Replicon chromosome
Accession NZ_CP044498
Organism Bacillus subtilis strain ms-2

Toxin (Protein)


Gene name spoIISA Uniprot ID O34853
Locus tag F8M43_RS06880 Protein ID WP_003244695.1
Coordinates 1304855..1305601 (-) Length 249 a.a.

Antitoxin (Protein)


Gene name spoIISB Uniprot ID G4EXD8
Locus tag F8M43_RS06875 Protein ID WP_003232646.1
Coordinates 1304685..1304855 (-) Length 57 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
F8M43_RS06840 1301551..1301877 + 327 WP_029727046.1 XkdW family protein -
F8M43_RS06845 1301874..1302038 + 165 WP_014479563.1 XkdX family protein -
F8M43_RS06850 1302082..1302921 + 840 WP_029727045.1 phage-like element PBSX protein XepA -
F8M43_RS06855 1302974..1303243 + 270 WP_015252265.1 hemolysin XhlA family protein -
F8M43_RS06860 1303256..1303519 + 264 WP_014479566.1 phage holin -
F8M43_RS06865 1303532..1304425 + 894 WP_029727044.1 N-acetylmuramoyl-L-alanine amidase -
F8M43_RS06870 1304462..1304599 - 138 WP_119899277.1 three component toxin-antitoxin-antitoxin system antitoxin SpoIISC -
F8M43_RS06875 1304685..1304855 - 171 WP_003232646.1 three component toxin-antitoxin-antitoxin system antitoxin SpoIISB Antitoxin
F8M43_RS06880 1304855..1305601 - 747 WP_003244695.1 toxin-antitoxin-antitoxin system toxin SpoIISA Toxin
F8M43_RS06885 1305711..1306712 - 1002 WP_003232642.1 inorganic phosphate transporter -
F8M43_RS06890 1306725..1307342 - 618 WP_003218470.1 DUF47 domain-containing protein -
F8M43_RS06895 1307618..1308934 - 1317 WP_015252262.1 serine/threonine exchanger -
F8M43_RS06900 1309322..1310272 + 951 WP_003232637.1 ring-cleaving dioxygenase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 1267624..1315501 47877


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin SpoIISA, type II toxin-antitoxin system

(3-238)

Antitoxin

Antitoxin SpoIISB

(1-56)


Sequences

Toxin        


Download         Length: 249 a.a.        Molecular weight: 29061.50 Da        Isoelectric Point: 4.6191

>T141023 WP_003244695.1 NZ_CP044498:c1305601-1304855 [Bacillus subtilis]
MVLFFQIMVWCIVAGLGLYVYATWRFEAKVKEKMSAIRKTWYLLFVLGAMVYWTYEPTSLFTHWERYLIVAVSFALIDAF
IFLSAYVKKLAGSELETDTREILEENNEMLHMYLNRLKTYQYLLKNEPIHVYYGSIDAYAEGIDKLLKTYADKMNLTASL
CHYSTQADKDRLTEHMDDPADVQTRLDRKDVYYDQYGKVVLIPFTIETQNYVIKLTSDSIVTEFDYLLFTSLTSIYDLVL
PIEEEGEG

Download         Length: 747 bp

>T141023 NZ_CP059711:c2768586-2768483 [Enterobacter hormaechei]
GGCAAGGCGATTGAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 57 a.a.        Molecular weight: 6654.72 Da        Isoelectric Point: 11.0203

>AT141023 WP_003232646.1 NZ_CP044498:c1304855-1304685 [Bacillus subtilis]
MERAFQNRCEPRAAKPFKILKKRSTTSVASYQVSPHTARIFKENERLIDEYKRKKA

Download         Length: 171 bp

>AT141023 NZ_CP059711:2768478-2768679 [Enterobacter hormaechei]
CAGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTT
GGCATTAATGCAGGCTCAATCGCCTTGCCCTTTAAGAATAGATGACGGCGTCAGGTTTTCCAGTCCACAGTAAAAGTGGT
CTGAAAAAAAGCGTCAGAACATCACTAAATGTGAAAAACCGC

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 3O6Q

Antitoxin

Source ID Structure
PDB 3O6Q
AlphaFold DB G4EXD8

References