Detailed information of TA system TA141022

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 516596..517232
• Replicon: chromosome
• Accession: NZ_CP044498
• Organism: Bacillus subtilis strain ms-2

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G4NU33
• Locus tag: F8M43_RS02640
• Protein ID: WP_003156187.1
• Coordinates: 516882..517232 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: G4NU32
• Locus tag: F8M43_RS02635
• Protein ID: WP_003225183.1
• Coordinates: 516596..516877 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F8M43_RS02615	512956..513555	-	600	WP_003246687.1	rhomboid family intramembrane serine protease	-
F8M43_RS02620	513650..514015	+	366	WP_015252768.1	holo-ACP synthase	-
F8M43_RS02625	514181..515197	+	1017	WP_072557167.1	outer membrane lipoprotein carrier protein LolA	-
F8M43_RS02630	515311..516480	+	1170	WP_029727188.1	alanine racemase	-
F8M43_RS02635	516596..516877	+	282	WP_003225183.1	type II toxin-antitoxin system antitoxin EndoAI	Antitoxin
F8M43_RS02640	516882..517232	+	351	WP_003156187.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
F8M43_RS02645	517348..518172	+	825	WP_009966610.1	RsbT co-antagonist protein RsbRA	-
F8M43_RS02650	518177..518542	+	366	WP_003225190.1	RsbT antagonist protein RsbS	-
F8M43_RS02655	518546..518947	+	402	WP_003246640.1	serine/threonine-protein kinase RsbT	-
F8M43_RS02660	518959..519966	+	1008	WP_003234295.1	phosphoserine phosphatase RsbU	-
F8M43_RS02665	520028..520357	+	330	WP_014475877.1	anti-sigma factor antagonist RsbV	-
F8M43_RS02670	520354..520836	+	483	WP_038427419.1	anti-sigma B factor RsbW	-
F8M43_RS02675	520802..521590	+	789	WP_003246715.1	RNA polymerase sigma factor SigB	-
F8M43_RS02680	521590..522189	+	600	WP_003234303.1	phosphoserine phosphatase RsbX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-45)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12977.98 Da
• Isoelectric Point: 4.8781

>T141022 WP_003156187.1 NZ_CP044498:516882-517232 [Bacillus subtilis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Nucleotide

• Length: 351 bp

>T141022 NZ_CP059711:c2333860-2333375 [Enterobacter hormaechei]
GTGGGACGTGTAACCGCCCCAGAGCCCTTATCCAGCGTCCATCAACTGGCGGAGTTCGTCAGTGGAGAGGCCGTACTTGA
CGAATGGTTAAAACAAAGAGGTCTGAAAAACCAGGCGCTTGCCGCAGCCCGAACGTTTGTCGTTTGTAAAACGGGCACAA
AGCAGGTCGCGGGATTTTACTCTCTGGCTACCGGCAGCGTTAACCATACGGAAGCGACAGGTAATTTGCGGCGTAACATG
CCGGATCCCATTCCCGTGATTATTCTTGCCCGTCTGGCTGTCGATGTCTCAATACGCGGAAAAGGGTTGGGAGCCGATTT
ACTCCAGGATGCGGTTTTACGCTGTTATCGGGTAGCAGAGAATATTGGGGTGCGGGCGATCATGGTTCATGCACTCACTG
AGGACGCCAAAGCGTTTTATATCCACCATGGTTTTAAAGCATCGCAGACTCAGGAACGGACACTGTTTCTCAGGCTTCCT
CAGTAG

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10546.92 Da
• Isoelectric Point: 6.4708

>AT141022 WP_003225183.1 NZ_CP044498:516596-516877 [Bacillus subtilis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG

Nucleotide

• Length: 282 bp

>AT141022 NZ_CP059711:c2334114-2333848 [Enterobacter hormaechei]
ATGAAATCAGATGTTCAACTCAATCTTAGAGCCAAAGAATCCCAGCGGGCGCTTATCGATGCCGCCGCAGAGATCCTCCA
TAAATCACGTACTGACTTCATTCTGGAGATGGCCTGTCAGGCAGCCGAAAATGTGATCCTCGATCGGCGTGTATTCAATT
TTAACGATGAACAGTATGCAGAGTTCATTGATATGCTTGATGCCCCGGTCGCCGACGATCCTGCCATAGAAAAACTGCTG
GCAAGGAAACCTCAGTGGGACGTGTAA

Structures

Toxin

• PDB: 4MDX
• PDB: 4ME7
• PDB: 1NE8

Antitoxin

• AlphaFold DB: G4NU32