Detailed information of TA system TA141008

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/upstrm_HI1419-dnstrm_HI1420
• Location: 3933..4523
• Replicon: plasmid pHZE30-1-4
• Accession: NZ_CP044487
• Organism: Acinetobacter schindleri strain HZE30-1

Toxin (Protein)

• Gene name: relE
• Uniprot ID: N8YGE2
• Locus tag: FSC11_RS15860
• Protein ID: WP_004787583.1
• Coordinates: 3933..4229 (+)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: FSC11_RS15865
• Protein ID: WP_004787581.1
• Coordinates: 4233..4523 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FSC11_RS15840	265..1206	+	942	WP_020846814.1	replication initiation protein	-
FSC11_RS15845	1203..1490	+	288	WP_004870774.1	hypothetical protein	-
FSC11_RS15850	1715..2902	-	1188	WP_046267530.1	Tet(A)/Tet(B)/Tet(C) family tetracycline efflux MFS transporter	-
FSC11_RS15855	2968..3609	+	642	WP_004787586.1	TetR/AcrR family transcriptional regulator C-terminal domain-containing protein	-
FSC11_RS15860	3933..4229	+	297	WP_004787583.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FSC11_RS15865	4233..4523	+	291	WP_004787581.1	putative addiction module antidote protein	Antitoxin
FSC11_RS15870	4857..5558	-	702	WP_004282341.1	IS1 family transposase	-
FSC11_RS15875	5787..7718	+	1932	WP_154320142.1	ATP-binding cassette domain-containing protein	-
FSC11_RS15880	7983..8684	+	702	WP_154320143.1	IS1 family transposase	-
FSC11_RS15885	8716..9108	-	393	Protein_9	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	tet(39)	-	1..14179	14179
	-	inside	IScluster/Tn	tet(39)	-	1715..8684	6969

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-89)

Antitoxin

• Uncharacterized protein HI1420 (9-94)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11598.27 Da
• Isoelectric Point: 6.4770

>T141008 WP_004787583.1 NZ_CP044487:3933-4229 [Acinetobacter schindleri]
MTEIKRLPEFDEWLDSIKDSMTRLKLNRRLDKVQRGNWGDVKHLQEGVWEMREFFGPGWRMYYIKHGDVIIVMLGGGDKS
TQQKDIERAVDLSKSLED

Nucleotide

• Length: 297 bp

>T141008 NZ_CP059675:c1801940-1801551 [Schaalia sp. JY-X169]
GTGATCATCGATACCTCAGCCATCATCGCGGTACTTCGCTCTGAAGATGAATCTGAGCGAATCGCTGAAGCGTTGGCAAG
CACCTCAGACTTGAGGATCTCTGCTGCAACGTACGTAGAGCTGTTCGCAGTCCTAAGTCGTTCGCGGGATCACACGATTG
CTGCCAGGCGGCTCCTTGACGATCTGGGTGCTACCGTGGAGCCCTTTGACCATGCTCAAGCGAAGATCGCATCATCCGCA
TACGTGCAATACGGGCGGGGAAGCGGGCATCGAGCAGGGCTCAACTTGGGGGACACTTATTCATATGCGCTTGCGTGGAT
CACGGGGCAGGAACTTTTGTTTGTTGGGGACGACTTCTCACACACCGATGTCCTCCCCGCACTCCGATAG

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10485.15 Da
• Isoelectric Point: 4.8096

>AT141008 WP_004787581.1 NZ_CP044487:4233-4523 [Acinetobacter schindleri]
MVKIADLPSFDMAEALKSEEDIVMYLNMVLEENDPAELAHALGVIAKARGMTQIAKEAGIGREALYKALRQDSAPRFDTI
NRVVNALGLKLTVQNA

Nucleotide

• Length: 291 bp

>AT141008 NZ_CP059675:c1802197-1801937 [Schaalia sp. JY-X169]
ATGAGTCTGAACATCAAGAATCAGGAGACGCATGCGAAGGTTCGCCGCCTCGCTGAACTCACAGGGGCAAGCCAAACTGC
TGCGGTCGATGACGCTGTGGAACGACGCCTCAGGGAGCTGGGCGCAGATCCCAATCTTGACCGTCGAGCTGAAGTCAGAG
CTGCGCTCGCAAGGTTCGCAGCTGCATTCTCGGATGAGGAAGCTCATGCCCTTCAGGAGGCTCAAGAGGCCCTCTACGAT
TCTGAAGGGTTGCCAGTGTGA

Structures

Toxin

• AlphaFold DB: A0A6C0XPU5