Detailed information of TA system TA141006

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumA-relB/upstrm_HI1419-dnstrm_HI1420
• Location: 33449..34033
• Replicon: plasmid pHZE30-1-1
• Accession: NZ_CP044484
• Organism: Acinetobacter schindleri strain HZE30-1

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: N8Q7V3
• Locus tag: FSC11_RS14985
• Protein ID: WP_000286964.1
• Coordinates: 33449..33751 (+)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: FSC11_RS14990
• Protein ID: WP_000985609.1
• Coordinates: 33752..34033 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FSC11_RS14945	28473..28904	-	432	WP_005108698.1	MarR family transcriptional regulator	-
FSC11_RS14950	28911..29339	-	429	WP_005108699.1	organic hydroperoxide resistance protein	-
FSC11_RS14955	29576..30910	-	1335	WP_015060273.1	ISNCY family transposase	-
FSC11_RS14960	31057..31371	-	315	WP_163167818.1	hypothetical protein	-
FSC11_RS14965	31368..31787	-	420	WP_163167820.1	hypothetical protein	-
FSC11_RS14970	32093..32380	+	288	WP_000438825.1	BrnT family toxin	-
FSC11_RS14975	32367..32681	+	315	WP_004728120.1	BrnA antitoxin family protein	-
FSC11_RS14980	32770..33300	-	531	WP_000172759.1	hypothetical protein	-
FSC11_RS14985	33449..33751	+	303	WP_000286964.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FSC11_RS14990	33752..34033	+	282	WP_000985609.1	putative addiction module antidote protein	Antitoxin
FSC11_RS14995	34113..34532	-	420	WP_001180106.1	SMI1/KNR4 family protein	-
FSC11_RS15000	34695..36029	+	1335	WP_015060273.1	ISNCY family transposase	-
FSC11_RS15005	36158..36478	+	321	WP_000897307.1	type II toxin-antitoxin system RelE/ParE family toxin	-
FSC11_RS15010	36471..36743	+	273	WP_000369781.1	helix-turn-helix domain-containing protein	-
FSC11_RS15020	38013..38828	+	816	WP_001043260.1	sulfonamide-resistant dihydropteroate synthase Sul2	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	blaNDM-1 / sul2 / aph(3'')-Ib / aph(6)-Id / aph(3')-VIa / floR / tet(X3) / tet(X6)	-	1..110146	110146
	-	inside	IScluster/Tn	blaNDM-1 / sul2	-	9493..40991	31498

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-90)

Antitoxin

• Uncharacterized protein HI1420 (4-91)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11573.07 Da
• Isoelectric Point: 4.6838

>T141006 WP_000286964.1 NZ_CP044484:33449-33751 [Acinetobacter schindleri]
MYSIYTTEVFDDWFTKLKDQQAKRRIQVRIDRVEDGNFGDTEPVGEGVSELRFFFGPGYRIYYCKQGQRVVILLAGGDKS
TQSKDIKLALQLAQDLEEEL

Nucleotide

• Length: 303 bp

>T141006 NZ_CP059591:c4760351-4760070 [Xanthomonas oryzae pv. oryzae]
GTGATCAGGAGCTTTATCGACAAGGACGCCGAAAAGATCTGGCTGGGTGAGCGTTCCCGCCGGTTGCCTGCCGACATCCA
ATTGGTCGCGCGCCGCAAGTTGCGCATGCTCAATGCCGCCGCACATCTCGACGATCTGCGGATCCCGCCCGCCAACCGGC
TGGAAGCGTTGAAGGGCCAGCGGCGCGGCCAGTACAGCATCCGGATCAACGATCAATGGCGCATCTGCTTCCGATGGATG
GAAGGCGATGTGGTCCAGGTCGAAATCGTCGATTACCACTGA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10109.67 Da
• Isoelectric Point: 8.0018

>AT141006 WP_000985609.1 NZ_CP044484:33752-34033 [Acinetobacter schindleri]
MAIKLRKWDSAEHLKTEDDMQAYLQACIEESNGDAAFIAKALGNIAKAKGMAQLSRDTGLGRESLYKALSGDVNPSFDTV
IKVVKALNLRLAI

Nucleotide

• Length: 282 bp

>AT141006 NZ_CP059591:c4760052-4759762 [Xanthomonas oryzae pv. oryzae]
ATGACGGTCCTACCCAATATCCATCCTGGCGACATTCTGCTTGAGGAGTTTCTCGAGCCTCTGGGCATCAGCCAGAATGC
ACTGGCACGCGCCACCGGCGTCCCGCCGCGCCGTATCAACGAAATCGTGCTGGGCAAGCGCGGCATCACTGCCGACACTG
CCGTGCGTCTGGCCGCCGCGTTGGGCACCTCCGAGCGCTTCTGGCTGGGCCTGCAGGCCGACTACGAGCTGGAACAGGCG
CATCGCGCGCTGGGGGATTTGCCGGCACGGATCAAGCGGCTGGCGGCGTGA

Structures

Toxin

• AlphaFold DB: A0A0A8TV94