Detailed information of TA system TA141001

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): AbkB-brnA/BrnT-BrnA
• Location: 1701695..1702283
• Replicon: chromosome
• Accession: NZ_CP044474
• Organism: Acinetobacter schindleri strain HZE33-1

Toxin (Protein)

• Gene name: AbkB
• Uniprot ID: -
• Locus tag: FSC12_RS08010
• Protein ID: WP_130114512.1
• Coordinates: 1701695..1701982 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: -
• Locus tag: FSC12_RS08015
• Protein ID: WP_163143224.1
• Coordinates: 1701969..1702283 (+)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FSC12_RS07975	1696829..1697386	+	558	Protein_1545	IS5-like element IS17 family transposase	-
FSC12_RS07980	1697610..1697924	+	315	WP_127800359.1	hypothetical protein	-
FSC12_RS07985	1697980..1698111	+	132	WP_127800375.1	transposase	-
FSC12_RS07990	1698242..1698481	-	240	WP_127800377.1	hypothetical protein	-
FSC12_RS07995	1698675..1699379	+	705	WP_099143705.1	IS6-like element IS1006 family transposase	-
FSC12_RS08000	1699787..1700053	+	267	WP_163168972.1	hypothetical protein	-
FSC12_RS08005	1700209..1701390	-	1182	WP_163168973.1	hypothetical protein	-
FSC12_RS08010	1701695..1701982	+	288	WP_130114512.1	BrnT family toxin	Toxin
FSC12_RS08015	1701969..1702283	+	315	WP_163143224.1	BrnA antitoxin family protein	Antitoxin
FSC12_RS08020	1702360..1703643	-	1284	WP_163168974.1	hypothetical protein	-
FSC12_RS08025	1703847..1705166	+	1320	WP_163168975.1	ISNCY family transposase	-
FSC12_RS08030	1705280..1705540	+	261	WP_163168976.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	-
FSC12_RS08035	1705527..1705817	+	291	Protein_1557	type II toxin-antitoxin system YafQ family toxin	-
FSC12_RS08040	1705898..1706455	-	558	WP_019836970.1	DNA-binding protein	-
FSC12_RS08045	1706919..1707197	+	279	Protein_1559	replication initiation protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1693188..1705166	11978
	-	inside	IScluster/Tn	-	-	1693950..1705166	11216

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (41-96)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11262.71 Da
• Isoelectric Point: 6.0900

>T141001 WP_130114512.1 NZ_CP044474:1701695-1701982 [Acinetobacter schindleri]
MKQYFEWDEAKNRKNQKKHDVSFETASLVFEDPLRISIQDRHTDGEERWQTIGKVKGVLMLLVAHTIFDEDDCEIIRIIS
ARQVTKAERDKYEHG

Nucleotide

• Length: 288 bp

>T141001 NZ_CP059591:430002-430154 [Xanthomonas oryzae pv. oryzae]
ATGAAGCGACTGCTGACACTGATGGTGCTGGGCCTGTTTTCGGCCGGCGTGATGACGGGCTGCAACACCGTGGCTGGCGC
TGGCAAGGACTTGCAGGGCGCGGGTGGGAAGGTCGAGAAAACGGCCGAAAAATGCAGCGATGGTAAGTGCTGA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 12199.83 Da
• Isoelectric Point: 8.4628

>AT141001 WP_163143224.1 NZ_CP044474:1701969-1702283 [Acinetobacter schindleri]
MSMVRYSRKELNEKFSDKQDAEIQRLLAKGTVPDDQLDLSDIPEVADWSNAVRQNQFYRPVKQQTSIRLDADVLAWFKAQ
GKGYQTRMNEILRDAMLKELKNHQ

Nucleotide

• Length: 315 bp

>AT141001 NZ_CP059591:430218-430352 [Xanthomonas oryzae pv. oryzae]
ATGAAGCGTGCAATTGTGCTGCTCGTGCTGTCCATGTTGTCGGTCGGTCTGCTGTCGGGCTGCAACACCGTCGCAGGTGC
CGGCAAGGACGTGAAGGGCGCCGGCGAGAAGGTGGAAGACGCAGCGCGTAAGTAA