Detailed information of TA system TA140991

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): AbkB-brnA/BrnT-BrnA
• Location: 2885413..2886001
• Replicon: chromosome
• Accession: NZ_CP044450
• Organism: Acinetobacter indicus strain MMS9-2

Toxin (Protein)

• Gene name: AbkB
• Uniprot ID: -
• Locus tag: FSC05_RS13810
• Protein ID: WP_130114512.1
• Coordinates: 2885714..2886001 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: -
• Locus tag: FSC05_RS13805
• Protein ID: WP_163143224.1
• Coordinates: 2885413..2885727 (-)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FSC05_RS13785	2880414..2881061	-	648	WP_163143216.1	hypothetical protein	-
FSC05_RS13790	2881175..2882299	-	1125	WP_163143219.1	SEL1-like repeat protein	-
FSC05_RS13795	2882510..2883844	+	1335	WP_015060273.1	ISNCY family transposase	-
FSC05_RS13800	2884011..2885336	+	1326	WP_163143509.1	ISNCY family transposase	-
FSC05_RS13805	2885413..2885727	-	315	WP_163143224.1	BrnA antitoxin family protein	Antitoxin
FSC05_RS13810	2885714..2886001	-	288	WP_130114512.1	BrnT family toxin	Toxin
FSC05_RS13815	2886258..2887583	-	1326	WP_127801714.1	ISNCY family transposase	-
FSC05_RS13820	2887725..2888054	-	330	WP_087545980.1	hypothetical protein	-
FSC05_RS13825	2888047..2888667	-	621	WP_087545982.1	hypothetical protein	-
FSC05_RS13830	2888816..2889082	+	267	WP_087545983.1	DUF4258 domain-containing protein	-
FSC05_RS13835	2889094..2889432	+	339	WP_087545985.1	hypothetical protein	-
FSC05_RS13840	2889821..2890525	-	705	WP_001067784.1	IS6-like element IS1006 family transposase	-
FSC05_RS13845	2890677..2890868	+	192	Protein_2670	VF530 family DNA-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2831679..2917029	85350
	-	inside	IScluster/Tn	-	-	2875103..2890525	15422

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (41-96)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11262.71 Da
• Isoelectric Point: 6.0900

>T140991 WP_130114512.1 NZ_CP044450:c2886001-2885714 [Acinetobacter indicus]
MKQYFEWDEAKNRKNQKKHDVSFETASLVFEDPLRISIQDRHTDGEERWQTIGKVKGVLMLLVAHTIFDEDDCEIIRIIS
ARQVTKAERDKYEHG

Nucleotide

• Length: 288 bp

>T140991 NZ_CP059589:c2985769-2985518 [Xanthomonas oryzae pv. oryzae]
ATGCTACGGCTGCGTGAATTCCTGAGGCCGAAGAATCCCGATGCCGCGAGGCGGGCCGGCGAAGCGATTAGGCGCGGCGT
CCAGGCTCTCGGTGCCCACCCTCGAATGGGGCGGCTCATAGAAGATCTGTCTGAACAGTATCGGGAGTGGCTTATCGATT
TTGGGGATAGCGGCTATGTGGCTCGGTACCGCTTCGATGGGGATACCGTCACGATTTTGGCCGTGCGACACCAGAAGGAA
GCGGGGTACTGA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 12199.83 Da
• Isoelectric Point: 8.4628

>AT140991 WP_163143224.1 NZ_CP044450:c2885727-2885413 [Acinetobacter indicus]
MSMVRYSRKELNEKFSDKQDAEIQRLLAKGTVPDDQLDLSDIPEVADWSNAVRQNQFYRPVKQQTSIRLDADVLAWFKAQ
GKGYQTRMNEILRDAMLKELKNHQ

Nucleotide

• Length: 315 bp

>AT140991 NZ_CP059589:c2986074-2985796 [Xanthomonas oryzae pv. oryzae]
ATGGTTACGGCAACTTCCATCAAGCTTGATGATGAATTGAAAGGGCGGGTTCAGCACTTGGCAGAGTCGCGCCGGCGTAC
CCCGCATTGGATCATGCGCGAAGCCATTGAGCAGTACGTCGAGCGCGAGGAAAAGCGCGAGGCGCTGAACCGGGACACGC
TCAAGGCATGGGATGAATTTCAGGCGACTGGCCTGCATGTGACCGCTGAGGAAGTGGACAAGTGGCTCGCGAGCTGGGGA
ACTGACGACGAACTGCCTCCGCCAGAATGCCACAAGTAA