Detailed information of TA system TA140985

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT-BrnA
• Location: 118680..119268
• Replicon: chromosome
• Accession: NZ_CP044445
• Organism: Acinetobacter indicus strain CMG3-2

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: -
• Locus tag: FSC02_RS00520
• Protein ID: WP_163169896.1
• Coordinates: 118981..119268 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: N9DAY7
• Locus tag: FSC02_RS00515
• Protein ID: WP_004282216.1
• Coordinates: 118680..118994 (-)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FSC02_RS00480	113869..114528	-	660	WP_163169890.1	hypothetical protein	-
FSC02_RS00485	114525..114944	-	420	WP_163169891.1	hypothetical protein	-
FSC02_RS00490	114922..115410	-	489	WP_163169892.1	prepilin-type N-terminal cleavage/methylation domain-containing protein	-
FSC02_RS00495	115404..115841	-	438	WP_163169893.1	prepilin-type N-terminal cleavage/methylation domain-containing protein	-
FSC02_RS00500	116164..116619	+	456	WP_163169894.1	helix-turn-helix domain-containing protein	-
FSC02_RS00505	116609..117175	+	567	WP_163170501.1	IS630 family transposase	-
FSC02_RS00510	117540..118568	-	1029	WP_163169895.1	hypothetical protein	-
FSC02_RS00515	118680..118994	-	315	WP_004282216.1	BrnA antitoxin family protein	Antitoxin
FSC02_RS00520	118981..119268	-	288	WP_163169896.1	BrnT family toxin	Toxin
FSC02_RS00525	119525..120850	-	1326	WP_163169897.1	ISNCY family transposase	-
FSC02_RS00530	120980..121957	-	978	WP_163169898.1	helix-turn-helix domain-containing protein	-
FSC02_RS00535	122090..122974	+	885	WP_005176778.1	MBL fold metallo-hydrolase	-
FSC02_RS00540	123058..123600	+	543	WP_163169899.1	cysteine hydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	wbtL	65656..152112	86456
	-	flank	IS/Tn	-	-	119525..120862	1337

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (41-96)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11276.69 Da
• Isoelectric Point: 5.6205

>T140985 WP_163169896.1 NZ_CP044445:c119268-118981 [Acinetobacter indicus]
MEQYFEWDEAKNRKNQKKHDISFETASLVFEDPLRISIQDRHTNGEERWQTIGKVKGVLMLLVAHTIFDEDDCEIIRIIS
ARQVTKAERDKYEHG

Nucleotide

• Length: 288 bp

>T140985 NZ_CP059588:1768089-1769012 [Xanthomonas oryzae pv. oryzae]
ATGAACGAACCTGACAAGAGCAGGCGCACTGTTGCCGCGTCGGTTCGCGCCCGGCTGCTCAACAAGGCCCGCGCCGAAAG
ACTCGACTTCAACCTGCTGCTGACCCGCTACGCGCTGGAGCGCGTCCTTTACCGCCTGAGCGTTTCGGAGCAGCAGGGTC
AATTCTTGCTCAAAGGTGCGCTGCTGTTCGACATCTGGTTCGACATACCGCATCGCCCGACGCACGATGCCGACCTGCTG
GGCTTCGGCTCCACCGAGATCCCACATCTGGAAGACCTGTTCCGCAGAATCAGTCAAATCGCCAGCGATGACGGCATCGT
GTTTCAGGCCGACAGCGTGAAGGCCGCCGAAATCCGCAAGGAGGCCAACTACGCGGGCGTCCGTGTGACGATGATCGGCC
TGCTCGATGGCGCGCGCTGCACGGTGCAGATCGACATCGGTTTCGGCGACGCCGTGACGCCCGGCCCGGACAACGTGCAG
TACCCAGTCATCCTCGACGGCATGCCGCAGCCGCAACTGCGGGTCTATCCCCGCTACACCGTGGTCGCCGAAAAGCTGGA
AGCGATGGTCAAGCTCGGCATCCTCAACAGCCGGATGAAGGACTACTTCGACCTCTGGGTTCTCGCCGGACATTCCGACT
TCGACGGTGCGGTGCTGGCCACGGCCATTCGCGCCACGTTCGAGTGCAGGGGTACGGCCATCCCACCCGGCGCGCCGCTG
GGCCTCACCGATGAGTTCGGCCTGGACGAGCAGAAGACAAAGCAGTGGCAGGCGTTCCTGCGCAAGAACACCTTGGAGCC
GATGCCGCTGACCACGGTGATCGAAGCGCTGCGCGAATTCGTGCTGCCCGTGCTGGCCGCGCTCGCGGCAGGCGAAGGTT
TCGACCGCCAGTGGCGCGCGGGCGCGGGATGGGATGCGGCCTGA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 12253.88 Da
• Isoelectric Point: 7.2029

>AT140985 WP_004282216.1 NZ_CP044445:c118994-118680 [Acinetobacter indicus]
MSMVRYSRKELNEKFSDKQDAEIERLLAKGTVPDDQLDLSDIPEITDWSNAVRHNQFYRPVKQQTSIRLDADVLAWFKAQ
GKGYQTRMNEILRDAMLKELKNHQ

Nucleotide

• Length: 315 bp

>AT140985 NZ_CP059588:1767493-1768092 [Xanthomonas oryzae pv. oryzae]
ATGGACACGACCATCGACAAGCTGCTGGAGCTGGTGCGCACGCGGGGACTTCTGCGCCCGCACGATCTGGCCCCACTGGG
CATCCCACGGGTGGCCCTGACGCGGGCCGTTCGGCGCGGGCAACTGGAGCGCGTCGGGCGCGGCCTGTACGGCCTGGTGG
CGCGTCCGGTGTCGGCCCACGGCACGCTGGCCGAAGTCGCGCGCCGCGTGCCCAAGGGCGTGGTCTGCCTGCTGTCGGCG
CTGCGCGTCCACGACCTGACGACCCAGGCCCCGTTCGAGGTGTGGCTGGCCATCGACAACAAGGCCGCTGCCCCGAAGCT
GGACTACCCGCCGCTACGTCTCGTGCGCTTCTCCGGGCCGGCGCTGAGCGAGGGCGTCGAGGAACACGTTGTCGATGGTG
TGCCCGTCCACGTGACCAGCGTCGCCAAGACCGTGGCCGACTGCTTCAAGTACCGCAACAAGATCGGCATCGACGTGGCG
CTGGAAGCGCTGCGCGAGGCGTGGGTTGCCCGGCGCATGACCAGCGACGAGATCTGGCACTACGCCAAGATCGACCGCGT
CGCCAACGTGATGCGTCCCTATCTGGAGAGCCTGGCATGA

Structures

Antitoxin

• AlphaFold DB: N9DAY7