Detailed information of TA system TA140980

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): spoIISABC/SpoIISA-SpoIISB
• Location: 2638241..2639158
• Replicon: chromosome
• Accession: NZ_CP044444
• Organism: Bacillus amyloliquefaciens strain KC41

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: I2HQ15
• Locus tag: F7V86_RS13815
• Protein ID: WP_007407256.1
• Coordinates: 2638412..2639158 (-)
• Length: 249 a.a.

Antitoxin (Protein)

• Gene name: spoIISB
• Uniprot ID: I2HQ14
• Locus tag: F7V86_RS13810
• Protein ID: WP_003154807.1
• Coordinates: 2638241..2638411 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F7V86_RS13765	2633448..2633960	+	513	WP_017417605.1	sigma-70 family RNA polymerase sigma factor	-
F7V86_RS13770	2634073..2634732	+	660	WP_065180407.1	terminase	-
F7V86_RS13775	2634630..2635097	+	468	Protein_2591	hypothetical protein	-
F7V86_RS13780	2635110..2635481	+	372	WP_017417608.1	hypothetical protein	-
F7V86_RS13785	2635486..2635683	+	198	WP_007610833.1	XkdX family protein	-
F7V86_RS13790	2635740..2636501	+	762	WP_017417609.1	hypothetical protein	-
F7V86_RS13795	2636552..2636815	+	264	WP_003154815.1	hemolysin XhlA family protein	-
F7V86_RS13800	2636829..2637092	+	264	WP_003154813.1	phage holin	-
F7V86_RS13805	2637106..2637984	+	879	WP_007407257.1	N-acetylmuramoyl-L-alanine amidase	-
F7V86_RS13810	2638241..2638411	-	171	WP_003154807.1	type II toxin-antitoxin system SpoIISB family antitoxin	Antitoxin
F7V86_RS13815	2638412..2639158	-	747	WP_007407256.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
F7V86_RS13820	2639262..2640260	-	999	WP_017417610.1	inorganic phosphate transporter	-
F7V86_RS13825	2640273..2640890	-	618	WP_003154804.1	DUF47 domain-containing protein	-
F7V86_RS13830	2641176..2642492	-	1317	WP_003154801.1	amino acid permease	-
F7V86_RS13835	2642815..2643765	+	951	WP_007610844.1	ring-cleaving dioxygenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (3-239)

Antitoxin

• Antitoxin SpoIISB (1-56)

Sequences

Toxin

Protein

• Length: 249 a.a.
• Molecular weight: 29077.57 Da
• Isoelectric Point: 4.7755

>T140980 WP_007407256.1 NZ_CP044444:c2639158-2638412 [Bacillus amyloliquefaciens]
MLLFFQIMVWTMAAALILYVYASWRYEAKVKEKMFAIRKTWYLLFVLGSMVYWTYDPESLFAAWRQYLIVAVCFALIDAF
IFLSAYIKKLAGNELETDTREILEENNEMLHSYLEKLKTYQYLLKNEPIHVYYGSTEAYAEGISRLLAAYAEKMNVTASL
CDYSAQSDKDRLTEHMPDAADVQSRLNRKDVYYDQKGRLVLIPFTVQNRHYVIKLTSENLLTEFDYLLFTSLTSIYDLML
PIEEEGDG

Nucleotide

• Length: 747 bp

>T140980 NZ_CP044444:c2639158-2638412 [Bacillus amyloliquefaciens]
ATGCTTTTGTTTTTTCAGATTATGGTGTGGACGATGGCGGCCGCACTCATTTTATATGTGTACGCATCCTGGCGGTATGA
GGCAAAGGTAAAAGAAAAAATGTTTGCCATCCGTAAAACGTGGTATTTACTATTTGTACTGGGCTCCATGGTCTATTGGA
CATATGATCCCGAATCGCTCTTTGCCGCATGGAGACAATATTTGATTGTTGCCGTCTGCTTTGCCCTTATTGATGCGTTT
ATCTTTTTGAGCGCGTATATCAAAAAGCTCGCCGGCAATGAATTAGAGACGGATACGCGGGAAATTTTAGAAGAAAATAA
CGAAATGCTTCACTCCTATCTTGAAAAACTGAAAACCTATCAATATCTGTTGAAAAATGAACCGATCCATGTATATTATG
GAAGTACAGAAGCTTATGCGGAAGGCATCAGCAGACTGCTGGCCGCTTATGCCGAAAAAATGAATGTCACGGCTTCCTTG
TGCGACTATTCCGCACAATCGGATAAGGACCGCTTGACGGAGCATATGCCTGATGCGGCAGACGTTCAGTCCCGTCTTAA
CCGTAAAGACGTCTACTATGATCAAAAAGGCAGGCTTGTGCTTATTCCGTTTACAGTCCAAAACCGCCATTACGTGATCA
AACTCACGTCGGAAAACCTGTTGACGGAATTCGATTATTTGCTTTTCACATCGCTAACGAGCATTTATGATTTAATGCTG
CCAATAGAAGAGGAAGGTGACGGTTAA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6676.74 Da
• Isoelectric Point: 10.9273

>AT140980 WP_003154807.1 NZ_CP044444:c2638411-2638241 [Bacillus amyloliquefaciens]
MEQAFQNGQPEQRAKPFKMFKKRSMTSIASYQVSPHTARIFKENERLIDKYKQKKA

Nucleotide

• Length: 171 bp

>AT140980 NZ_CP044444:c2638411-2638241 [Bacillus amyloliquefaciens]
ATGGAGCAGGCATTTCAAAACGGGCAGCCGGAACAAAGAGCGAAACCGTTCAAAATGTTTAAAAAACGTTCAATGACAAG
TATCGCAAGTTACCAGGTCAGCCCTCATACCGCCAGAATTTTCAAAGAAAACGAGCGGCTGATTGACAAGTATAAACAAA
AAAAAGCTTGA

Structures

Toxin

• AlphaFold DB: I2HQ15

Antitoxin

• AlphaFold DB: I2HQ14