Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 1851066..1851703 Replicon chromosome
Accession NZ_CP044444
Organism Bacillus amyloliquefaciens strain KC41

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag F7V86_RS09605 Protein ID WP_003156187.1
Coordinates 1851353..1851703 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID I2HMS5
Locus tag F7V86_RS09600 Protein ID WP_003156188.1
Coordinates 1851066..1851347 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
F7V86_RS09580 1847431..1848030 - 600 WP_003156193.1 rhomboid family intramembrane serine protease -
F7V86_RS09585 1848123..1848488 + 366 WP_007609580.1 holo-ACP synthase -
F7V86_RS09590 1848653..1849660 + 1008 WP_007410230.1 outer membrane lipoprotein carrier protein LolA -
F7V86_RS09595 1849777..1850946 + 1170 WP_065180972.1 alanine racemase -
F7V86_RS09600 1851066..1851347 + 282 WP_003156188.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
F7V86_RS09605 1851353..1851703 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
F7V86_RS09610 1851821..1852642 + 822 WP_003156182.1 STAS domain-containing protein -
F7V86_RS09615 1852647..1853012 + 366 WP_007609584.1 RsbT antagonist protein RsbS -
F7V86_RS09620 1853015..1853416 + 402 WP_003156178.1 anti-sigma regulatory factor -
F7V86_RS09625 1853428..1854435 + 1008 WP_007609589.1 PP2C family protein-serine/threonine phosphatase -
F7V86_RS09630 1854499..1854828 + 330 WP_003156176.1 anti-sigma factor antagonist RsbV -
F7V86_RS09635 1854825..1855307 + 483 WP_007609591.1 anti-sigma B factor RsbW -
F7V86_RS09640 1855273..1856061 + 789 WP_003156171.1 RNA polymerase sigma factor SigB -
F7V86_RS09645 1856061..1856663 + 603 WP_007410234.1 SpoIIE family protein phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T140979 WP_003156187.1 NZ_CP044444:1851353-1851703 [Bacillus amyloliquefaciens]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

>T140979 NZ_CP059587:1669154-1670077 [Xanthomonas oryzae pv. oryzae]
ATGAACGAACCTGACAAGAGCAGGCGCACTGTTGCCGCGTCGGTTCGCGCCCGGCTGCTCAACAAGGCCCGCGCCGAAAG
ACTCGACTTCAACCTGCTGCTGACCCGCTACGCGCTGGAGCGCGTCCTTTACCGCCTGAGCGTTTCGGAGCAGCAGGGTC
AATTCTTGCTCAAAGGTGCGCTGCTGTTCGACATCTGGTTCGACATACCGCATCGCCCGACGCACGATGCCGACCTGCTG
GGCTTCGGCTCCACCGAGATCCCACATCTGGAAGACCTGTTCCGCAGAATCAGTCAAATCGCCAGCGATGACGGCATCGT
GTTTCAGGCCGACAGCGTGAAGGCCGCCGAAATCCGCAAGGAGGCCAACTACGCGGGCGTCCGTGTGACGATGATCGGCC
TGCTCGATGGCGCGCGCTGCACGGTGCAGATCGACATCGGTTTCGGCGACGCCGTGACGCCCGGCCCGGACAACGTGCAG
TACCCAGTCATCCTCGACGGCATGCCGCAGCCGCAACTGCGGGTCTATCCCCGCTACACCGTGGTCGCCGAAAAGCTGGA
AGCGATGGTCAAGCTCGGCATCCTCAACAGCCGGATGAAGGACTACTTCGACCTCTGGGTTCTCGCCGGACATTCCGACT
TCGACGGTGCGGTGCTGGCCACGGCCATTCGCGCCACGTTCGAGTGCAGGGGTACGGCCATCCCACCCGGCGCGCCGCTG
GGCCTCACCGATGAGTTCGGCCTGGACGAGCAGAAGACAAAGCAGTGGCAGGCGTTCCTGCGCAAGAACACCTTGGAGCC
GATGCCGCTGACCACGGTGATCGAAGCGCTGCGCGAATTCGTGCTGCCCGTGCTGGCCGCGCTCGCGGCAGGCGAAGGTT
TCGACCGCCAGTGGCGCGCGGGCGCGGGATGGGATGCGGCCTGA

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10562.92 Da        Isoelectric Point: 6.4706

>AT140979 WP_003156188.1 NZ_CP044444:1851066-1851347 [Bacillus amyloliquefaciens]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Download         Length: 282 bp

>AT140979 NZ_CP059587:1668558-1669157 [Xanthomonas oryzae pv. oryzae]
ATGGACACGACCGTCGACAAGCTGCTGGAGCTGGTGCGTACGCGGGGACTTCTGCGCCCGCACGATCTGGCCCCACTGGG
CATCCCACGGGTGGCCCTGACGCGGGCCGTTCGGCGCGGGCAACTGGAGCGCGTCGGGCGCGGCCTGTACGGCCTGGTGG
CGCGTCCGGTGTCGGCCCACGGCACGCTGGCCGAAGTCGCGCGCCGCGTGCCCAAGGGCGTGGTCTGCCTGCTGTCGGCG
CTGCGCGTCCACGACCTGACGACCCAGGCCCCGTTCGAGGTGTGGCTGGCCATCGACAACAAGGCCGCTGCCCCGAAGCT
GGACTACCCGCCGCTACGTCTCGTGCGCTTCTCCGGGCCGGCGCTGAGCGAGGGCGTCGAGGAACACGTTGTCGATGGTG
TGCCCGTCCACGTGACCAGCGTCGCCAAGACCGTGGCCGACTGCTTCAAGTACCGCAACAAGATCGGCATCGACGTGGCG
CTGGAAGCGCTGCGCGAGGCGTGGGTTGCCCGGCGCATGACCAGCGACGAGATCTGGCACTACGCCAAGATCGACCGCGT
CGCCAACGTGATGCGTCCCTATCTGGAGAGCCTGGCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
AlphaFold DB I2HMS5

References