Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4145273..4145770 Replicon chromosome
Accession NZ_CP044443
Organism Escherichia coli strain HeN100

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag HeN100_RS22050 Protein ID WP_001173343.1
Coordinates 4145273..4145446 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag HeN100_RS22055 Protein ID WP_000398619.1
Coordinates 4145474..4145770 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HeN100_RS22040 (4141943) 4141943..4143400 + 1458 WP_089570918.1 dipeptide/tripeptide permease DtpC -
HeN100_RS22045 (4143637) 4143637..4145154 + 1518 WP_001295074.1 lysine--tRNA ligase -
HeN100_RS22050 (4145273) 4145273..4145446 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
HeN100_RS22055 (4145474) 4145474..4145770 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
HeN100_RS22060 (4145997) 4145997..4146269 - 273 WP_000405651.1 GNAT family N-acetyltransferase -
HeN100_RS22065 (4146281) 4146281..4146511 - 231 WP_000371704.1 4Fe-4S mono-cluster protein YjdI -
HeN100_RS22070 (4146692) 4146692..4148323 + 1632 WP_001216477.1 sensor histidine kinase -
HeN100_RS22075 (4148320) 4148320..4149039 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 4103471..4145770 42299


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T140978 WP_001173343.1 NZ_CP044443:c4145446-4145273 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T140978 NZ_CP059587:434524-434658 [Xanthomonas oryzae pv. oryzae]
ATGAAGCGTGCAATTGTGCTGCTCGTGCTGTCCATGTTGTCGGTCGGTCTGCTGTCGGGCTGCAACACCGTCGCAGGTGC
CGGCAAGGACGTGAAGGGCGCCGGCGAGAAGGTGGAAGACGCAGCGCGTAAGTAA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT140978 WP_000398619.1 NZ_CP044443:c4145770-4145474 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT140978 NZ_CP059587:434308-434460 [Xanthomonas oryzae pv. oryzae]
ATGAAGCGACTGCTGACACTGATGGTGCTGGGCCTGTTTTCGGCCGGCGTGATGACGGGCTGCAACACCGTGGCTGGCGC
TGGCAAGGACTTGCAGGGCGCGGGTGGGAAGGTCGAGAAAACGGCCGAAAAATGCAGCGATGGTAAGTGCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References