Detailed information of TA system TA140977

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 4133490..4134325
• Replicon: chromosome
• Accession: NZ_CP044443
• Organism: Escherichia coli strain HeN100

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A7W4PV54
• Locus tag: HeN100_RS22005
• Protein ID: WP_000854821.1
• Coordinates: 4133948..4134325 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: HeN100_RS22000
• Protein ID: WP_032216526.1
• Coordinates: 4133490..4133858 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HeN100_RS21960 (4128618)	4128618..4129502	+	885	WP_032216515.1	50S ribosome-binding GTPase	-
HeN100_RS21965 (4129621)	4129621..4130298	+	678	WP_001097364.1	hypothetical protein	-
HeN100_RS21970 (4130304)	4130304..4130537	+	234	WP_001278282.1	DUF905 family protein	-
HeN100_RS21975 (4130636)	4130636..4131454	+	819	WP_113403051.1	DUF932 domain-containing protein	-
HeN100_RS21980 (4131546)	4131546..4132031	+	486	WP_032216512.1	antirestriction protein	-
HeN100_RS21985 (4132047)	4132047..4132523	+	477	WP_001186773.1	RadC family protein	-
HeN100_RS21990 (4132586)	4132586..4132807	+	222	WP_076486783.1	DUF987 domain-containing protein	-
HeN100_RS21995 (4132826)	4132826..4133471	+	646	Protein_3973	antitoxin of toxin-antitoxin stability system	-
HeN100_RS22000 (4133490)	4133490..4133858	+	369	WP_032216526.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
HeN100_RS22005 (4133948)	4133948..4134325	+	378	WP_000854821.1	type IV toxin-antitoxin system cytoskeleton-binding toxin CbtA	Toxin
HeN100_RS22010 (4134322)	4134322..4134471	+	150	Protein_3976	DUF5983 family protein	-
HeN100_RS22015 (4134547)	4134547..4134744	+	198	WP_087904847.1	DUF957 domain-containing protein	-
HeN100_RS22020 (4134829)	4134829..4135671	+	843	WP_153519512.1	DUF4942 domain-containing protein	-
HeN100_RS22025 (4136420)	4136420..4137958	+	1539	WP_001187173.1	lysine decarboxylation/transport transcriptional activator CadC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	4103471..4145770	42299

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (19-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13930.95 Da
• Isoelectric Point: 8.5163

>T140977 WP_000854821.1 NZ_CP044443:4133948-4134325 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPGAKQ

Nucleotide

• Length: 378 bp

>T140977 NZ_CP059587:434308-434460 [Xanthomonas oryzae pv. oryzae]
ATGAAGCGACTGCTGACACTGATGGTGCTGGGCCTGTTTTCGGCCGGCGTGATGACGGGCTGCAACACCGTGGCTGGCGC
TGGCAAGGACTTGCAGGGCGCGGGTGGGAAGGTCGAGAAAACGGCCGAAAAATGCAGCGATGGTAAGTGCTGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13810.69 Da
• Isoelectric Point: 6.2066

>AT140977 WP_032216526.1 NZ_CP044443:4133490-4133858 [Escherichia coli]
VSDKLHETNYPDDHNDRLWWGLLCTVTPCFGARLVQEGNRLHYLVDRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT140977 NZ_CP059587:434524-434658 [Xanthomonas oryzae pv. oryzae]
ATGAAGCGTGCAATTGTGCTGCTCGTGCTGTCCATGTTGTCGGTCGGTCTGCTGTCGGGCTGCAACACCGTCGCAGGTGC
CGGCAAGGACGTGAAGGGCGCCGGCGAGAAGGTGGAAGACGCAGCGCGTAAGTAA

Structures

Toxin

• AlphaFold DB: A0A7W4PV54