Detailed information of TA system TA140976

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-RelB
• Location: 4103796..4104394
• Replicon: chromosome
• Accession: NZ_CP044443
• Organism: Escherichia coli strain HeN100

Toxin (Protein)

• Gene name: doc
• Uniprot ID: -
• Locus tag: HeN100_RS21845
• Protein ID: WP_053270478.1
• Coordinates: 4103796..4104173 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: L4J1C0
• Locus tag: HeN100_RS21850
• Protein ID: WP_001603498.1
• Coordinates: 4104173..4104394 (-)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HeN100_RS21815 (4099020)	4099020..4099358	+	339	WP_000883400.1	divalent cation tolerance protein CutA	-
HeN100_RS21820 (4099334)	4099334..4101031	+	1698	WP_112922321.1	protein-disulfide reductase DsbD	-
HeN100_RS21825 (4101068)	4101068..4101643	+	576	WP_001188520.1	transcriptional regulator	-
HeN100_RS21835 (4102023)	4102023..4103285	+	1263	WP_076486553.1	integrase arm-type DNA-binding domain-containing protein	-
HeN100_RS21840 (4103471)	4103471..4103686	+	216	Protein_3942	transposase	-
HeN100_RS21845 (4103796)	4103796..4104173	-	378	WP_053270478.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
HeN100_RS21850 (4104173)	4104173..4104394	-	222	WP_001603498.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
HeN100_RS21855 (4104579)	4104579..4105589	-	1011	WP_089615833.1	fimbrial protein	-
HeN100_RS21860 (4105600)	4105600..4108086	-	2487	WP_153519524.1	fimbrial biogenesis outer membrane usher protein	-
HeN100_RS21865 (4108104)	4108104..4108784	-	681	WP_053270475.1	fimbrial assembly chaperone	-
HeN100_RS21870 (4108840)	4108840..4109388	-	549	WP_076486551.1	fimbrial protein YehD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	4103471..4145770	42299
	-	flank	IS/Tn	-	-	4103471..4103752	281

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Fido domain (4-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-51)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13419.20 Da
• Isoelectric Point: 6.7260

>T140976 WP_053270478.1 NZ_CP044443:c4104173-4103796 [Escherichia coli]
MRHISPEELIAIHDANISRYGGLPGMSDSGRAEAIIGRVQARVAYEEITGLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVYDSPELAELTVGAATGEVSVSTVVATLRRLYGTA

Nucleotide

• Length: 378 bp

>T140976 NZ_CP059586:c4760024-4759743 [Xanthomonas oryzae pv. oryzae]
GTGATCAGGAGCTTTATCGACAAGGACGCCGAAAAGATCTGGCTGGGTGAGCGTTCCCGCCGGTTGCCTGCCGACATCCA
ATTGGTCGCGCGCCGCAAGTTGCGCATGCTCAATGCCGCCGCACATCTCGACGATCTGCGGATCCCGCCCGCCAACCGGC
TGGAAGCGTTGAAGGGCCAGCGGCGCGGCCAGTACAGCATCCGGATCAACGATCAATGGCGCATCTGCTTCCGATGGATG
GAAGGCGATGTGGTCCAGGTCGAAATCGTCGATTACCACTGA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8190.24 Da
• Isoelectric Point: 6.3159

>AT140976 WP_001603498.1 NZ_CP044443:c4104394-4104173 [Escherichia coli]
MQSINFRTARSNLTEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDTTNKKLVNR

Nucleotide

• Length: 222 bp

>AT140976 NZ_CP059586:c4759725-4759435 [Xanthomonas oryzae pv. oryzae]
ATGACGGTCCTACCCAATATCCATCCTGGCGACATTCTGCTTGAGGAGTTTCTCGAGCCTCTGGGCATCAGCCAGAATGC
ACTGGCACGCGCCACCGGCGTCCCGCCGCGCCGTATCAACGAAATCGTGCTGGGCAAGCGCGGCATCACTGCCGACACTG
CCGTGCGTCTGGCCGCCGCGTTGGGCACCTCCGAGCGCTTCTGGCTGGGCCTGCAGGCCGACTACGAGCTGGAACAGGCG
CATCGCGCGCTGGGGGATTTGCCGGCACGGATCAAGCGGCTGGCGGCGTGA

Structures

Antitoxin

• AlphaFold DB: L4J1C0