Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 832710..833364 Replicon chromosome
Accession NZ_CP044443
Organism Escherichia coli strain HeN100

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag HeN100_RS05820 Protein ID WP_000244777.1
Coordinates 832957..833364 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag HeN100_RS05815 Protein ID WP_000354046.1
Coordinates 832710..832976 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HeN100_RS05790 (827879) 827879..828622 + 744 WP_000951962.1 SDR family oxidoreductase -
HeN100_RS05795 (828679) 828679..830112 - 1434 WP_001338826.1 6-phospho-beta-glucosidase BglA -
HeN100_RS05800 (830157) 830157..830468 + 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
HeN100_RS05805 (830632) 830632..831291 + 660 WP_000250274.1 hemolysin III family protein -
HeN100_RS05810 (831487) 831487..832467 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
HeN100_RS05815 (832710) 832710..832976 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
HeN100_RS05820 (832957) 832957..833364 + 408 WP_000244777.1 protein YgfX Toxin
HeN100_RS05825 (833404) 833404..833925 - 522 WP_001055874.1 flavodoxin FldB -
HeN100_RS05830 (834037) 834037..834933 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
HeN100_RS05835 (834958) 834958..835668 + 711 WP_089570894.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
HeN100_RS05840 (835674) 835674..837407 + 1734 WP_000813212.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T140959 WP_000244777.1 NZ_CP044443:832957-833364 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T140959 NZ_CP059583:c3070735-3070484 [Xanthomonas oryzae pv. oryzae]
ATGCTACGGCTGCGTGAATTCCTGAGGCCGAAGAATCCCGATGCCGCGAGGCGGGCCGGCGAAGCGATTAGGCGCGGCGT
CCAGGCTCTCGGTGCCCACCCTCGAATGGGGCGGCTCATAGAAGATCTGTCTGAACAGTATCGGGAGTGGCTTATCGATT
TTGGGGATAGCGGCTATGTGGCTCGGTACCGCTTCGATGGGGATACCGTCACGATTTTGGCCGTGCGACACCAGAAGGAA
GCGGGGTACTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT140959 WP_000354046.1 NZ_CP044443:832710-832976 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT140959 NZ_CP059583:c3071040-3070762 [Xanthomonas oryzae pv. oryzae]
ATGGTTACGGCAACTTCCATCAAGCTTGATGATGAATTGAAAGGGCGGGTTCAGCACTTGGCAGAGTCGCGCCGGCGTAC
CCCGCATTGGATCATGCGCGAAGCCATTGAGCAGTACGTCGAGCGCGAGGAAAAGCGCGAGGCGCTGAACCGGGACACGC
TCAAGGCATGGGATGAATTTCAGGCGACTGGCCTGCATGTGACCGCTGAGGAAGTGGACAAGTGGCTCGCGAGCTGGGGA
ACTGACGACGAACTGCCTCCGCCAGAATGCCACAAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References