Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-Phd
Location 71948..72549 Replicon plasmid pHeN100_02
Accession NZ_CP044439
Organism Escherichia coli strain HeN100

Toxin (Protein)


Gene name doc Uniprot ID F4TND7
Locus tag HeN100_RS01000 Protein ID WP_001216030.1
Coordinates 71948..72328 (-) Length 127 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID U9YQH9
Locus tag HeN100_RS01005 Protein ID WP_001190712.1
Coordinates 72328..72549 (-) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HeN100_RS00970 67051..67173 - 123 Protein_71 ash family protein -
HeN100_RS00975 (HeN100_01050) 67389..68873 - 1485 WP_000124159.1 hypothetical protein -
HeN100_RS00980 (HeN100_01055) 68873..70066 - 1194 WP_024233185.1 hypothetical protein -
HeN100_RS00985 (HeN100_01060) 70152..70604 - 453 WP_001326849.1 late promoter-activating protein -
HeN100_RS00990 (HeN100_01065) 70693..71736 - 1044 WP_032153800.1 DUF968 domain-containing protein -
HeN100_RS00995 (HeN100_01070) 71764..71943 - 180 WP_000113018.1 hypothetical protein -
HeN100_RS01000 (HeN100_01075) 71948..72328 - 381 WP_001216030.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
HeN100_RS01005 (HeN100_01080) 72328..72549 - 222 WP_001190712.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
HeN100_RS01010 (HeN100_01085) 72622..72816 - 195 WP_050516570.1 hypothetical protein -
HeN100_RS01015 (HeN100_01090) 73077..76883 - 3807 WP_153519545.1 autotransporter outer membrane beta-barrel domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..99733 99733
- flank IS/Tn - - 77341..77550 209


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Fido domain

(4-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-51)


Sequences

Toxin        


Download         Length: 127 a.a.        Molecular weight: 13601.29 Da        Isoelectric Point: 5.1408

>T140952 WP_001216030.1 NZ_CP044439:c72328-71948 [Escherichia coli]
MRHISPEELIALHDANINRYGGLPGMSDPGRAEAIIGRVQARVAYEDITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVADTLRRLYGSAE

Download         Length: 381 bp

>T140952 NZ_CP059582:1578112-1579035 [Xanthomonas oryzae pv. oryzae]
ATGAACGAACCTGACAAGAGCAGGCGCACTGTTGCCGCGTCGGTTCGCGCCCGGCTGCTCAACAAGGCCCGCGCCGAAAG
ACTCGACTTCAACCTGCTGCTGACCCGCTACGCGCTGGAGCGCGTCCTTTACCGCCTGAGCGTTTCGGAGCAGCAGGGTC
AATTCTTGCTCAAAGGTGCGCTGCTGTTCGACATCTGGTTCGACATACCGCATCGCCCGACGCACGATGCCGACCTGCTG
GGCTTCGGCTCCACTGAGATCCCACATCTGGAAGACCTGTTCCGCAGAATCAGTCAAATCGCCAGCGATGACGGCATCGT
GTTTCAGGCCGACAGCGTGAAGGCCGCCGAAATCCGCAAGGAGGCCAACTACGCGGGCGTCCGTGTGACGATGATCGGCC
TGCTCGATGGCGCGCGCTGCACGGTGCAGATCGACATCGGTTTCGGCGACGCCGTGACGCCCGGCCCGGACAACGTGCAG
TACCCAGTCATCCTCGACGGCATGCCGCAGCCGCAACTGCGGGTCTATCCCCGCTACACCGTGGTCGCCGAAAAGCTGGA
AGCGATGGTCAAGCTCGGCATCCTCAACAGCCGGATGAAGGACTACTTCGACCTCTGGGTTCTCGCCGGACATTCCGACT
TCGACGGTGCGGTGCTGGCCACGGCCATTCGCGCCACGTTCGAGTGCAGGGGTACGGCCATCCCACCCGGCGCGCCGCTG
GGCCTCACCGATGAGTTCGGCCTGGACGAGCAGAAGACAAAGCAGTGGCAGGCGTTCCTGCGCAAGAACACCTTGGAGCC
GATGCCGCTGACCACGGTGATCGAAGCGCTGCGCGAATTCGTGCTGCCCGTGCTGGCCGCGCTCGCGGCAGGCGAAGGTT
TCGACCGCCAGTGGCGCGCGGGCGCGGGATGGGATGCGGCCTGA

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8133.10 Da        Isoelectric Point: 4.7708

>AT140952 WP_001190712.1 NZ_CP044439:c72549-72328 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Download         Length: 222 bp

>AT140952 NZ_CP059582:1577516-1578115 [Xanthomonas oryzae pv. oryzae]
ATGGACACGACCGTCGACAAGCTGCTGGAGCTGGTGCGTACGCGGGGACTTCTGCGCCCGCACGATCTGGCCCCACTGGG
CATCCCACGGGTGGCCCTGACGCGGGCCGTTCGGCGCGGGCAACTGGAGCGCGTCGGGCGCGGCCTGTACGGCCTGGTGG
CGCGTCCGGTGTCGGCCCACGGCACGCTGGCCGAAGTCGCGCGCCGCGTGCCCAAGGGCGTGGTCTGCCTGCTGTCGGCG
CTGCGCGTCCACGACCTGACGACCCAGGCCCCGTTCGAGGTGTGGCTGGCCATCGACAACAAGGCCGCTGCCCCGAAGCT
GGACTACCCGCCGCTACGTCTCGTGCGCTTCTCCGGGCCGGCGCTGAGCGAGGGCGTCGAGGAACACGTTGTCGATGGTG
TGCCCGTCCACGTGACCAGCGTCGCCAAGACCGTGGCCGACTGCTTCAAGTACCGCAACAAGATCGGCATCGACGTGGCG
CTGGAAGCGCTGCGCGAGGCGTGGGTTGCCCGGCGCATGACCAGCGACGAGATCTGGCACTACGCCAAGATCGACCGCGT
CGCCAACGTGATGCGTCCCTATCTGGAGAGCCTGGCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1M1W8Q3

Antitoxin

Source ID Structure
AlphaFold DB A0A829CJB6

References