Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-DnaT
Location 71732..72254 Replicon plasmid pHeN100_01
Accession NZ_CP044438
Organism Escherichia coli strain HeN100

Toxin (Protein)


Gene name relE Uniprot ID G3CAN5
Locus tag HeN100_RS00385 Protein ID WP_000220560.1
Coordinates 71973..72254 (+) Length 94 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID G3CAG1
Locus tag HeN100_RS00380 Protein ID WP_000121743.1
Coordinates 71732..71983 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HeN100_RS00355 (HeN100_00370) 67403..67684 + 282 WP_001185482.1 hypothetical protein -
HeN100_RS00360 (HeN100_00375) 67709..68686 + 978 WP_024245156.1 hypothetical protein -
HeN100_RS00365 (HeN100_00380) 68683..69039 - 357 WP_000456533.1 DNA distortion polypeptide 3 -
HeN100_RS00370 (HeN100_00385) 69176..69622 - 447 Protein_73 hypothetical protein -
HeN100_RS00375 (HeN100_00390) 69662..70498 - 837 WP_001050931.1 RepB family plasmid replication initiator protein -
HeN100_RS00380 (HeN100_00405) 71732..71983 + 252 WP_000121743.1 plasmid stabilization protein Antitoxin
HeN100_RS00385 (HeN100_00410) 71973..72254 + 282 WP_000220560.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
HeN100_RS00390 (HeN100_00415) 72329..73026 + 698 WP_103215986.1 IS1-like element IS1A family transposase -
HeN100_RS00395 (HeN100_00420) 73036..73344 + 309 Protein_78 replication initiation protein -
HeN100_RS00400 (HeN100_00430) 73437..74141 - 705 WP_001067855.1 IS6-like element IS26 family transposase -
HeN100_RS00405 (HeN100_00435) 74186..74554 - 369 Protein_80 IS6 family transposase -
HeN100_RS00410 (HeN100_00440) 74821..76257 + 1437 WP_000105383.1 glutathione synthase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid tet(A) / dfrA12 / aadA2 / cmlA1 / ant(3'')-Ia / tet(M) / aph(3')-Ia / mph(A) / qnrS1 / oqxA / oqxB / floR / blaTEM-1B - 1..114090 114090


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 94 a.a.        Molecular weight: 11005.83 Da        Isoelectric Point: 10.5938

>T140951 WP_000220560.1 NZ_CP044438:71973-72254 [Escherichia coli]
MTYELAFDRRALKEWQKLGHTIREQFKKKLAERLENPRVPAARLHGHADRYKIKLRASGYRLVYQVIDEKVVLLVIAVGR
RESSEVYQIADLR

Download         Length: 282 bp

>T140951 NZ_CP059582:427590-427724 [Xanthomonas oryzae pv. oryzae]
ATGAAGCGTGCAATTGTGCTGCTCGTGCTGTCCATGTTGTCGGTCGGTCTGCTGTCGGGCTGCAACACCGTCGCAGGTGC
CGGCAAGGACGTGAAGGGCGCCGGCGAGAAGGTGGAAGACGCAGCGCGTAAGTAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9194.43 Da        Isoelectric Point: 4.1683

>AT140951 WP_000121743.1 NZ_CP044438:71732-71983 [Escherichia coli]
MSYQILTTTAASITDLKKNPMGTVAEGEGDAVAILNRNEPAFYCVPPKLYAYYRELAEDAELNAVADERMKNPEIVKVNL
DDL

Download         Length: 252 bp

>AT140951 NZ_CP059582:427374-427526 [Xanthomonas oryzae pv. oryzae]
ATGAAGCGACTGCTGACACTGATGGTGCTGGGCCTGTTTTCGGCCGGCGTGATGACGGGCTGCAACACCGTGGCTGGCGC
TGGCAAGGACTTGCAGGGCGCGGGTGGGAAGGTCGAGAAAACGGCCGAAAAATGCAGCGATGGTAAGTGCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB G3CAN5

Antitoxin

Source ID Structure
AlphaFold DB A0A3S5I301

References