Detailed information of TA system TA140944

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PP_4151-4152/excise(antitoxin)
• Location: 3241449..3242470
• Replicon: chromosome
• Accession: NZ_CP044437
• Organism: Proteus mirabilis strain C74

Toxin (Protein)

• Gene name: PP_4152
• Uniprot ID: A0A8E4FRK4
• Locus tag: F7R85_RS15155
• Protein ID: WP_017827532.1
• Coordinates: 3241901..3242470 (+)
• Length: 190 a.a.

Antitoxin (Protein)

• Gene name: PP_4151
• Uniprot ID: B4F2H3
• Locus tag: F7R85_RS15150
• Protein ID: WP_004245528.1
• Coordinates: 3241449..3241901 (+)
• Length: 151 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F7R85_RS15130	3237235..3237765	-	531	WP_004245524.1	RNA pyrophosphohydrolase	-
F7R85_RS15135	3238034..3239047	+	1014	WP_004250228.1	thiamine ABC transporter substrate binding subunit	-
F7R85_RS15140	3239047..3240627	+	1581	WP_104731821.1	thiamine/thiamine pyrophosphate ABC transporter permease ThiP	-
F7R85_RS15145	3240620..3241321	+	702	WP_004245527.1	thiamine ABC transporter ATP-binding protein ThiQ	-
F7R85_RS15150	3241449..3241901	+	453	WP_004245528.1	helix-turn-helix domain-containing protein	Antitoxin
F7R85_RS15155	3241901..3242470	+	570	WP_017827532.1	PIN domain-containing protein	Toxin
F7R85_RS15160	3242586..3244940	+	2355	WP_107337099.1	DNA polymerase II	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-113)

Antitoxin

• SinI-like, DNA-binding domain (80-125)

Sequences

Toxin

Protein

• Length: 190 a.a.
• Molecular weight: 21601.08 Da
• Isoelectric Point: 7.0180

>T140944 WP_017827532.1 NZ_CP044437:3241901-3242470 [Proteus mirabilis]
MTNYTVLLDACVMYPAPLRSYLMYLANTGLFRARWSNQIHDEWITNLLVNNPTLTAERLIRTRDLMNAHVPDCLVEGYEP
LICGLKLPDPNDRHVLAAAIKGQAQAIITFNLKDFPQKYLNSFDISAIHPDEFLSDMFELNPSSCIKAAQQHRQSLKDPP
MTVETFLGCLQKQKLPQFISHLRAFQILL

Nucleotide

• Length: 570 bp

>T140944 NZ_CP059581:1284348-1284677 [Xanthomonas oryzae pv. oryzae]
TTGCATCATGTCGCCGAACGCCGATGGCCGTTTCTGTTTACTGACAGCCACGCCTACTGCCAGTTGGCGAGCTTCTATTC
CGATTTATCTGATCTCGACAAGATCGACTGGCCGTTGCTCCAAGCACGCGATTTCCGGCGCGACCCAGACGACCCAGCCA
AGTTCGAGCGCTATCAGGCCGAGGCCCTGATTCACCGACACTTGCCGCTTGATGGCCTGCGCGGCATTGTGTGTCATACG
GATGGTTTGAAACAGACCATCGAGCGGCAACTCCAAGAGCGAAACCTGAAATTGCCGGTTCATACCCGCACCGATTGGTA
TTTCCGATGA

Antitoxin

Protein

• Length: 151 a.a.
• Molecular weight: 16700.15 Da
• Isoelectric Point: 5.7723

>AT140944 WP_004245528.1 NZ_CP044437:3241449-3241901 [Proteus mirabilis]
MNTISERLSAEDIALAKLSSQELSAVMDINGKTQKLSIIDKTGNNHIVQIPNSALRLMIEILTQLGQGNTVSITPIHAEL
TTQEAADILNMSRPTFVKLLDTRAIPYSRKGNRRKVAYADIVAYKNELEQKRLDALAELSQLDQEMDMGY

Nucleotide

• Length: 453 bp

>AT140944 NZ_CP059581:1284674-1285093 [Xanthomonas oryzae pv. oryzae]
ATGATCACGTTTACACAAGGCAATCTACTGGAGTCTGGCGCAGAGGCGCTGGTCAACACCGTCAACACCGTCGGCGTGAT
GGGCAAGGGCATCGCGCTGATGTTCAAGGAGCGCTTCAAGGAGAACTTCCTCCGATATGCCGCCGCCTGCAAAGACAACC
AATTGCGCATTGGCAAAATTTTCGTCACCGAAGTCAACGAACTGGACGGGCCACGCTGGATCATCAATTTTCCGACCAAA
CAGCACTGGCGTGGCGATTCCCGCATCGAATGGATCACTGAAGGATTGCAGGACTTGCACCGTTTTCTGATCGAGAACAA
AGTCAAATCCATTGCCATCCCGCCACTGGGCGCGGGCAATGGAGGATTGGACTGGGCCGAGGTGCGCCCACTGATCGAAG
AAGTACTAGGGAACCTCTGA

Structures

Antitoxin

• AlphaFold DB: B4F2H3