Detailed information of TA system TA140939

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): VapXD/-
• Location: 3443586..3444056
• Replicon: chromosome
• Accession: NZ_CP044436
• Organism: Proteus mirabilis strain C55

Toxin (Protein)

• Gene name: VapD
• Uniprot ID: A0A8E4FU70
• Locus tag: F7R84_RS16020
• Protein ID: WP_060555119.1
• Coordinates: 3443778..3444056 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: VapX
• Uniprot ID: A0A8E4FTC4
• Locus tag: F7R84_RS16015
• Protein ID: WP_060555126.1
• Coordinates: 3443586..3443774 (+)
• Length: 63 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F7R84_RS15980	3438894..3440093	-	1200	WP_049218680.1	lipoprotein-releasing ABC transporter permease subunit LolC	-
F7R84_RS15985	3440395..3440796	+	402	WP_049218678.1	PPC domain-containing protein	-
F7R84_RS15990	3441213..3441614	-	402	WP_060555124.1	phage holin family protein	-
F7R84_RS15995	3441696..3442049	-	354	WP_060555123.1	hypothetical protein	-
F7R84_RS16000	3442121..3442369	-	249	WP_060555122.1	DUF883 family protein	-
F7R84_RS16005	3442819..3443166	+	348	WP_060555121.1	type II toxin-antitoxin system RelE/ParE family toxin	-
F7R84_RS16010	3443166..3443465	+	300	WP_060555120.1	XRE family transcriptional regulator	-
F7R84_RS16015	3443586..3443774	+	189	WP_060555126.1	DUF5397 family protein	Antitoxin
F7R84_RS16020	3443778..3444056	+	279	WP_060555119.1	virulence factor	Toxin
F7R84_RS16025	3444535..3446025	+	1491	WP_060555118.1	YadA-like family protein	-
F7R84_RS16030	3446212..3446286	-	75	Protein_3106	IS200/IS605 family transposase	-
F7R84_RS16035	3446280..3446387	-	108	Protein_3107	transposase	-
F7R84_RS16040	3446437..3446643	-	207	WP_060555117.1	hypothetical protein	-
F7R84_RS16045	3446640..3447263	-	624	WP_060555116.1	ParA family protein	-
F7R84_RS16050	3447732..3448250	-	519	WP_060555115.1	ClbS/DfsB family four-helix bundle protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	tet(J) / blaCMY-2	-	3300638..3487444	186806

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Virulence-associated protein D / CRISPR associated protein Cas2 (1-86)

Antitoxin

• Protein of unknown function DUF5397 (5-62)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10589.85 Da
• Isoelectric Point: 6.4606

>T140939 WP_060555119.1 NZ_CP044436:3443778-3444056 [Proteus mirabilis]
MYAISFDLTVADTEKHHPKGVSQAYNDISSTLKRYGFERIQGSLYTNSDENMANLFQAMNALKALEWFPNSVRDIRAFRI
EQWSDFTSSMKG

Nucleotide

• Length: 279 bp

>T140939 NZ_CP059572:4112806-4113180 [Actinomadura graeca]
GTGTCCTGGGACGTCATCCTCCTCGAACCAGTCGAGAGCTGGTTCCTCAAGCTCTGCGAATCCGATGTGGACACCGCAGC
CTTGATCGAGCAGGCCATCGACAGGCTGGCCGTAGTAGGACCGACACTGGGACGGCCGCTCGTCGACACCCTGGAACACA
GCGAACTGCGCAACCTCAAGGAACTGCGACCCGGCAGCCGCGGGCGATCGGAGATCCGCATGCTGTTCGTCTTCGATCCA
GACCGCGCCGCGATCTTCCTCGTGGCCGGAGACAAAGCCGGGCAGTGGTCCCGGTGGTACGACCAGGCCATCCCACTGGC
CGAAGCCCGCTACAGCGAATACCGGGCCGCGAGAGACAAGGAGGCCGGACGATGA

Antitoxin

Protein

• Length: 63 a.a.
• Molecular weight: 6787.78 Da
• Isoelectric Point: 3.9020

>AT140939 WP_060555126.1 NZ_CP044436:3443586-3443774 [Proteus mirabilis]
MNTVQIPTGSIKTFGLFGIPYEVGEPAQELPDGDILVNIILVQTGEKELYKLSKLLQDPDAE

Nucleotide

• Length: 189 bp

>AT140939 NZ_CP059572:4113177-4113506 [Actinomadura graeca]
ATGAGCACCGGACGCCGCTGGCAGGACATCAAGGCCGAAGCCCACCACCGCAACCCCGAACTGGCCGACCCCCAGCGGCA
AGCCCAGGCCCGTGCCGAACTGGACGCCTATGTCGCCGGCCACCACCTCAAGGAACTTCGCAAGGCCATCGGCAAGACAC
AAGCCGAGATCGCCCAGATCCTCGGCGTCTCCCAGTCCCGCGTCTCCCAGATCGAGAACGGCGACCTTGAAGCCATGGAG
CTGGAAACGCTCCGCGCCTACGCCACCGCACTCGGCGGACACCTCGATATCACCGTCAGCGTCGGCCCCCACTCCATCAA
GGTCGCCTGA