Detailed information of TA system TA140930

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 124943..125478
• Replicon: plasmid pPAN2
• Accession: NZ_CP044425
• Organism: Paracoccus pantotrophus strain DSM 2944

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: A0A1I5P2D9
• Locus tag: ESD82_RS08470
• Protein ID: WP_024846182.1
• Coordinates: 125179..125478 (+)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: A0A1I5P292
• Locus tag: ESD82_RS08465
• Protein ID: WP_028711008.1
• Coordinates: 124943..125179 (+)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ESD82_RS08435	120406..121260	+	855	WP_147429434.1	ABC transporter permease	-
ESD82_RS08440	121257..122861	+	1605	WP_123130383.1	ABC transporter ATP-binding protein	-
ESD82_RS08445	122872..123813	+	942	WP_167521739.1	MurR/RpiR family transcriptional regulator	-
ESD82_RS08450	123930..124142	-	213	Protein_116	transposase domain-containing protein	-
ESD82_RS08455	124427..124620	-	194	Protein_117	phage terminase large subunit family protein	-
ESD82_RS08460	124656..124859	-	204	Protein_118	sugar isomerase	-
ESD82_RS08465	124943..125179	+	237	WP_028711008.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
ESD82_RS08470	125179..125478	+	300	WP_024846182.1	CcdB family protein	Toxin
ESD82_RS08475	125942..126178	-	237	WP_147429351.1	hypothetical protein	-
ESD82_RS08480	126191..126400	+	210	WP_147429350.1	transposase domain-containing protein	-
ESD82_RS08485	126421..126576	+	156	Protein_123	TonB-dependent receptor	-
ESD82_RS08490	126616..127360	+	745	Protein_124	ABC transporter substrate-binding protein	-
ESD82_RS08495	127550..128974	+	1425	WP_147429349.1	iron chelate uptake ABC transporter family permease subunit	-
ESD82_RS08500	129141..129404	-	264	WP_147277965.1	hypothetical protein	-
ESD82_RS08505	129600..130268	-	669	WP_024844137.1	ABC transporter ATP-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	aph(3'')-Ib / aph(6)-Id	glf / glf	1..535332	535332

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (3-98)

Antitoxin

• Post-segregation antitoxin CcdA (8-78)

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 10669.40 Da
• Isoelectric Point: 4.1564

>T140930 WP_024846182.1 NZ_CP044425:125179-125478 [Paracoccus pantotrophus]
MARYDVYASPDGSGYLLDVQTDLLDNLSTRLVVPMMPVGASPPVLKQLNPIFDIQSERYVMVTQFMAAVPASILKTLAGS
LAQHDTEITGALDMLLTGV

Nucleotide

• Length: 300 bp

>T140930 NZ_CP059569:1809878-1810177 [Kingella oralis]
ATGGAAAAAAGAACGCCGCATTGCAGCCTAGAAAGAGTAAAAGCCTTGTTGGCAGAAGAAAAAGTCCGCGCAACCCGTGT
AGCACTGGTTGGTGCGGAGCTTTTAGGCATTGATTATGACGAAATGCAAAATATCGTGAGCAACCTAAACCGTTCAGACT
TCTATAAAAGCATGACCACTCATGCAGACCATACAATTTGGCAAGATGTATATCGCCCAACCGTTGAGCCCTACGGCGAT
TTGTATATCAAGCTAACCGTAATAGACGATGTTTTAATAGTTTCATTCAAGGAGCGTTAA

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 8753.95 Da
• Isoelectric Point: 10.2690

>AT140930 WP_028711008.1 NZ_CP044425:124943-125179 [Paracoccus pantotrophus]
MQTQSVPKKPTNLSLDQGLLSEARSFGVNLSQAAEAGLRRAVREAKAEAWKRENAKAIEGVNRWVEENGLPLEKYRLF

Nucleotide

• Length: 237 bp

>AT140930 NZ_CP059569:1810183-1810581 [Kingella oralis]
ATGAAATGTCCCGTGTGTGGCGCAGCAGAATTAGTGCACGATGTGCGCGATATTCCTTATACCTACAAAGGGAAAACAAC
CATTATTCCCCAAGTAGAAGCGGATTTTTGCCCTGCGTGTGGCGAATCGCTAACCGCGCCTGATGAAAGCCGCCGAATGA
TGAATTTAATGGGCGAATTTCATAAACAAGTAAACAATGAAATCGCTAGCCCCAGCTTTATCCGCAATGTTCGCGCCAAA
CTTAATTTAAGCCAACGCGAAGCGGGCGAACTGTTTGGCGGTGGCGTAAATGCTTTTTCTCGCTACGAAACAGGTAAAGC
AGCCCCACCAAAATTGTTGGTTATGATGTTTAAAATGCTGGATAGCAATCCTAGCCTTTTAGAGCAGTTGCGGAACTAA

Structures

Toxin

• AlphaFold DB: A0A1I5P2D9

Antitoxin

• AlphaFold DB: A0A1I5P292