Detailed information of TA system TA140927

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): omega-epsilon-zeta/zeta-epsilon
• Location: 17969..19167
• Replicon: plasmid unnamed1
• Accession: NZ_CP044413
• Organism: Lactobacillus sp. JM1

Toxin (Protein)

• Gene name: zeta
• Uniprot ID: -
• Locus tag: F3I00_RS10290
• Protein ID: WP_159311103.1
• Coordinates: 18244..19167 (+)
• Length: 308 a.a.

Antitoxin (Protein)

• Gene name: epsilon
• Uniprot ID: V5E259
• Locus tag: F3I00_RS10285
• Protein ID: WP_007124950.1
• Coordinates: 17969..18241 (+)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F3I00_RS10240	12989..13273	-	285	Protein_16	type II toxin-antitoxin system RelB/DinJ family antitoxin	-
F3I00_RS10245	13540..13656	+	117	Protein_17	IS3 family transposase	-
F3I00_RS10250	13663..14115	-	453	WP_021349374.1	hypothetical protein	-
F3I00_RS10260	14360..14452	+	93	Protein_19	recombinase family protein	-
F3I00_RS10265	14587..15378	+	792	WP_159311101.1	AAA family ATPase	-
F3I00_RS10270	15450..15689	+	240	WP_010011603.1	hypothetical protein	-
F3I00_RS10275	16245..17414	+	1170	WP_159311102.1	replication initiator protein A	-
F3I00_RS10280	17389..17685	+	297	WP_023487876.1	hypothetical protein	-
F3I00_RS10285	17969..18241	+	273	WP_007124950.1	epsilon-antitoxin	Antitoxin
F3I00_RS10290	18244..19167	+	924	WP_159311103.1	zeta toxin family protein	Toxin
F3I00_RS10295	19157..19435	-	279	WP_159311104.1	hypothetical protein	-
F3I00_RS10300	19458..19685	-	228	WP_201288371.1	hypothetical protein	-
F3I00_RS10305	19939..22002	+	2064	WP_159311106.1	MobA/MobL family protein	-
F3I00_RS10310	22086..22397	+	312	WP_003712158.1	hypothetical protein	-
F3I00_RS10315	22434..23048	+	615	WP_159311107.1	hypothetical protein	-
F3I00_RS10320	23050..23385	+	336	WP_012552810.1	CagC family type IV secretion system protein	-
F3I00_RS10325	23406..23768	+	363	WP_003590770.1	conjugal transfer protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..44217	44217

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Zeta toxin domain (23-213)

Antitoxin

• Bacterial epsilon antitoxin (4-90)

Sequences

Toxin

Protein

• Length: 308 a.a.
• Molecular weight: 35502.18 Da
• Isoelectric Point: 6.2682

>T140927 WP_159311103.1 NZ_CP044413:18244-19167 [Lactobacillus sp. JM1]
MDELADYTDHQFETKLQIEIEQLTKNKQAQESPKAYLLGGQPGAGKSGLHQLIKAEDPNAITIDNDTFKWLHPKYKQLEQ
KYGKDVVKYVTPFSNQMTESLIDYLSDKKFDLIIEGTLRTVEVPMATVTKLQNRGYEASLYVMAVPRIESYLGTLARYED
QFSLSPRTARATTKEAHDVVARQLPDNLDFLYKQRLFKEIRLYDRKGNKLYSSLENLNESPKKIITEILNRKLDNNTLLN
SIDSVINKMEINHHTTTPQYLDLVEKTTELKKEISGRVQEQLKEFAEKNPEVNPKEDPENKNDRPSY

Nucleotide

• Length: 924 bp

>T140927 NZ_CP059568:10955-11233 [Kingella oralis]
ATGTACGCAATTTCTTTTGATTTGGTGGTTGCTGATACCGCTGAAAAACACCCGAAAGGCGTTTCACAAGCCTATGCGGA
TATTGGCGCTACTCTACGCAAATTCGGTTTTGAGCGTGTGCAGGGCAGTTTGTACACCTGCCAAGATGAGAATATGGCTA
ATCTGTTTTCTGCCATCTCTGCGCTGAAACTGCTGCCGTGGTTTCCTGATTCAGTCCGTGATATTCGGGCGTTCCGCATT
GAACAATGGTCGGATTTCACAGGGTTTGTGAAGTCTTAA

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10340.91 Da
• Isoelectric Point: 5.5093

>AT140927 WP_007124950.1 NZ_CP044413:17969-18241 [Lactobacillus sp. JM1]
MKLDYQQTYKKEILTEFASSIYAKVVNLVVDQELNIHDESHFLVKLMHQLGDAKLVIMDAHSLGELETIQAYWQAMNNFI
DSLPTKSKVA

Nucleotide

• Length: 273 bp

>AT140927 NZ_CP059568:10761-10955 [Kingella oralis]
ATGCAACCAACAGTAAACACTATCCCAACAGGCTCTATCCGTACTTTCGGCGATTACGGCGTAAAGTACATTGTCGGCAC
ACCTGCTAAACAACTTCCTGATGGCGATTGGCTCGTAAATATTGAGCTGCCTGAAAGCGGAGAACACACGCAATACAAAC
TTTCCCATATTGTCCAAGACCCGAAAGCTGCATAA

Structures

Antitoxin

• AlphaFold DB: V5E259