Detailed information of TA system TA140926

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/PRK09907-MazE
• Location: 10219..10820
• Replicon: plasmid unnamed1
• Accession: NZ_CP044413
• Organism: Lactobacillus sp. JM1

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: U6FEM0
• Locus tag: F3I00_RS10215
• Protein ID: WP_011241744.1
• Coordinates: 10219..10563 (-)
• Length: 115 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: -
• Locus tag: F3I00_RS10220
• Protein ID: WP_049160264.1
• Coordinates: 10557..10820 (-)
• Length: 88 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F3I00_RS10195	5957..6742	-	786	WP_007124627.1	hydroxyethylthiazole kinase	-
F3I00_RS10200	7197..8223	+	1027	Protein_7	hypothetical protein	-
F3I00_RS10730	8215..8715	+	501	Protein_8	type II CRISPR RNA-guided endonuclease Cas9	-
F3I00_RS10205	8726..8941	+	216	WP_003590798.1	hypothetical protein	-
F3I00_RS10210	8945..10061	+	1117	Protein_10	hypothetical protein	-
F3I00_RS10215	10219..10563	-	345	WP_011241744.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
F3I00_RS10220	10557..10820	-	264	WP_049160264.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
F3I00_RS10225	10916..11140	+	225	Protein_13	hypothetical protein	-
F3I00_RS10230	11245..11829	+	585	WP_159311099.1	recombinase family protein	-
F3I00_RS10235	12152..12778	-	627	WP_159311100.1	hypothetical protein	-
F3I00_RS10240	12989..13273	-	285	Protein_16	type II toxin-antitoxin system RelB/DinJ family antitoxin	-
F3I00_RS10245	13540..13656	+	117	Protein_17	IS3 family transposase	-
F3I00_RS10250	13663..14115	-	453	WP_021349374.1	hypothetical protein	-
F3I00_RS10260	14360..14452	+	93	Protein_19	recombinase family protein	-
F3I00_RS10265	14587..15378	+	792	WP_159311101.1	AAA family ATPase	-
F3I00_RS10270	15450..15689	+	240	WP_010011603.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..44217	44217

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-101)

Antitoxin

• SpoVT-AbrB domain (12-55)

Sequences

Toxin

Protein

• Length: 115 a.a.
• Molecular weight: 13010.79 Da
• Isoelectric Point: 5.5556

>T140926 WP_011241744.1 NZ_CP044413:c10563-10219 [Lactobacillus sp. JM1]
MVSQGDIFYVNFNPSRGHEQMNRRPAIALSNDLVCQTSNMTIVAPISSTERNFPMYHRLTSSQTVYGKVLLDQTIALDLR
ARHVADEAIVDHVSREELEEIITLYKLLFSIDDK

Nucleotide

• Length: 345 bp

>T140926 NZ_CP059567:c2307651-2307397 [Neisseria shayeganii]
ATGAAGAAGAGACACCAGCGCACGCTTGCTTTGATATTCAGCCGCCCCGTATCCGGTGGCATCAAATGGGCGGATATAGA
ATCCCTGTTTACCGAGCTGGGCGCGGAAATTCAGGAGCGGGAAGGCAGTCGCATTGCCGTTGTGCTATTTGGGCAAGTGA
AAGTGTTTCATCGGCCGCACCCCAGTCCCGATACCGACAAAGGCGCGGTGGCTAGCGTGCGCAAATGGTTGGAAGACAAC
GGAGTAAAACCATGA

Antitoxin

Protein

• Length: 88 a.a.
• Molecular weight: 10169.48 Da
• Isoelectric Point: 5.7117

>AT140926 WP_049160264.1 NZ_CP044413:c10820-10557 [Lactobacillus sp. JM1]
MELIKEQTRLAKWGNSKAARIPSQIIRQLKLDDNQDMTITIENGSIVLTPIKKNPTNIHELFKDWQDDGKRDHELDWGKS
EGNELQW

Nucleotide

• Length: 264 bp

>AT140926 NZ_CP059567:c2307400-2307065 [Neisseria shayeganii]
ATGAACAACATAATGGAAATCAACGGCCATAAGGCAGTTATCCAGTACGACCCGGAAACCGAATTGCTGCGCGGAGAATT
TATCGGCCTCAATGGCGGGGCGGACTTTTACGCCGACAACGTGGCCGACCTGCACAAAGAAGGCGCAGCCAGCTTGGCGA
TATTTCTCGATGTGTGCAAAGAGAAAGGCATTGAGCCTTACAAGCAGTATTCAGGCCGTTTCAATGTACGGGTAAGCCCC
GCTACCCATGCGGCAGCAGTAGCGGCAGCAGCCGCGCAGAATATCAGCTTGAACGAGTGGGTAAGCCGTGCCATCAGCCA
GTCTGCTCAGTCCTGA

Structures

Toxin

• AlphaFold DB: U6FEM0