Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 2214843..2215481 Replicon chromosome
Accession NZ_CP044410
Organism Escherichia coli strain ecMN1F

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag FNW43_RS10725 Protein ID WP_000813794.1
Coordinates 2214843..2215019 (+) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag FNW43_RS10730 Protein ID WP_001270286.1
Coordinates 2215065..2215481 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FNW43_RS10705 2210462..2211676 - 1215 WP_001326689.1 BenE family transporter YdcO -
FNW43_RS10710 2211729..2212265 + 537 WP_000429155.1 DNA-binding transcriptional regulator SutR -
FNW43_RS10715 2212338..2214299 + 1962 WP_001303492.1 23S rRNA 5-hydroxycytidine C2501 synthase -
FNW43_RS10720 2214391..2214621 - 231 WP_000494244.1 YncJ family protein -
FNW43_RS10725 2214843..2215019 + 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
FNW43_RS10730 2215065..2215481 + 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
FNW43_RS10735 2215560..2216966 + 1407 WP_000760626.1 PLP-dependent aminotransferase family protein -
FNW43_RS10740 2217211..2218356 + 1146 WP_000047424.1 ABC transporter substrate-binding protein -
FNW43_RS10745 2218374..2219387 + 1014 WP_000220396.1 ABC transporter ATP-binding protein -
FNW43_RS10750 2219388..2220329 + 942 WP_001251304.1 ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T140891 WP_000813794.1 NZ_CP044410:2214843-2215019 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T140891 NZ_CP059497:483573-483923 [Bacillus velezensis]
TTGATTGTAAAGCGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGCGTACGCCCGGT
TTTAGTGATCCAAAATGATATCGGAAATCGCTTTAGCCCGACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATTGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGATTAACGGATAAAATAACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCCT
GCAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT140891 WP_001270286.1 NZ_CP044410:2215065-2215481 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT140891 NZ_CP059497:483286-483567 [Bacillus velezensis]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACTTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGGACAACTCGCCATAACC
GTGATTTGATGAGACGCGGGTATATGGAAATGGCGAAAATCAACTTGAATATCTCTTCTGAGGCTCACTATGCTGAGTGC
GAAGCAGAAACGACCGTTGAGCGCTTAGTCAGCGGAGGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References