Detailed information of TA system TA140879

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yafW-ykfI/CbtA-CbeA
• Location: 969917..970596
• Replicon: chromosome
• Accession: NZ_CP044410
• Organism: Escherichia coli strain ecMN1F

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: P77692
• Locus tag: FNW43_RS04545
• Protein ID: WP_000854672.1
• Coordinates: 969917..970258 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yafW
• Uniprot ID: Q47684
• Locus tag: FNW43_RS04550
• Protein ID: WP_000070395.1
• Coordinates: 970279..970596 (-)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FNW43_RS04520	965194..965595	+	402	WP_000174689.1	sigma factor-binding protein Crl	-
FNW43_RS04525	965634..966689	-	1056	WP_000749863.1	phosphoporin PhoE	-
FNW43_RS04530	966977..968080	+	1104	WP_001285288.1	glutamate 5-kinase	-
FNW43_RS04535	968092..969345	+	1254	WP_000893278.1	glutamate-5-semialdehyde dehydrogenase	-
FNW43_RS04545	969917..970258	-	342	WP_000854672.1	type IV toxin-antitoxin system toxin YkfI	Toxin
FNW43_RS04550	970279..970596	-	318	WP_000070395.1	type IV toxin-antitoxin system antitoxin YafW	Antitoxin
FNW43_RS04555	970615..970836	-	222	WP_000691994.1	DUF987 domain-containing protein	-
FNW43_RS04560	970845..971321	-	477	WP_000811693.1	RadC family protein	-
FNW43_RS04565	971337..971795	-	459	WP_000211838.1	antirestriction protein	-
FNW43_RS04570	971893..972132	-	240	WP_000194654.1	DUF905 domain-containing protein	-
FNW43_RS04575	972209..972676	-	468	WP_001547765.1	hypothetical protein	-
FNW43_RS04580	972699..973142	-	444	WP_000824223.1	hypothetical protein	-
FNW43_RS04585	973142..973369	-	228	WP_001548158.1	hypothetical protein	-
FNW43_RS04590	973365..973556	-	192	Protein_873	DeoR family transcriptional regulator	-
FNW43_RS04595	973773..974594	-	822	WP_000197389.1	DUF945 domain-containing protein	-
FNW43_RS04600	974686..975549	-	864	WP_001065553.1	GTPase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (6-104)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12905.01 Da
• Isoelectric Point: 9.6543

>T140879 WP_000854672.1 NZ_CP044410:c970258-969917 [Escherichia coli]
MKTLPAITQRAVKPCLSPVAVWQMLLTRLLEQHYGLTINDTPFCNEAVIKEHIDAGITLADAVNFLVEKYELVRIDRKGF
SWQEQSPYLRAADILRARQATGLLRQSRNNVVR

Nucleotide

• Length: 342 bp

>T140879 NZ_CP059488:2709627-2709920 [Acidithiobacillus ferrivorans]
ATCAGCATCAAAACGACGGCGATTTTTGATCGCTGGCTCCATGGCCTGCGGGATCGCAGGGCGAGGGTGCGTGTTCAGGT
GCGCATTGATCGCATGGAGTTGGCCCATTTTGGCGACTGTGAACCTGTCGGAGAAGGCGTATCCGAAATGCGTATCCATT
ACGGTCCCGGCTATCGGGTGTACTTCAAGCGGTTTGGCCTGGAACTCGTCGTGCTTCTGGCCGGAGGCGACAAATCCACG
CAATCGGCCGACATTGCCCTGGCATTGAAGTTGGCACGACAACTGGAGGGGTGA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11939.59 Da
• Isoelectric Point: 6.7391

>AT140879 WP_000070395.1 NZ_CP044410:c970596-970279 [Escherichia coli]
MSNPTRGLQREITLRLGARLVQEGNRLHYLADRASITGKFSDIECRKLDETFPHFILQMESMLTTGELSPHHAHCVTLYH
NDLTCEADTLGSCGYVYIAIYPTQR

Nucleotide

• Length: 318 bp

>AT140879 NZ_CP059488:2709924-2710223 [Acidithiobacillus ferrivorans]
ATGGAAAAATTGGAATTGCACACATGGGACAGTGCCGAGCACCTGAAAACCGAAGAGGACATCGCGGCTTATCTCAACGC
CTGTTTTGAAGAGGCGGGGGATGACGCGGCCTTCATCGCCAGGGCGCTGGGTACCGTTGCCCGCGCACGCGGCATGACCC
GACTGGCCCATGACGCCGGCATGGGCAGGGAAAGCCTCTACAAAGCCCTTTCCGGAGAAGGCAACCCCAGTTTTGCCACC
ATTCTCAAGGTAGCCAAAGCCTTGGGTGTGCGCATTCGCGCCGAAGCTCTGCACGTCTGA

Structures

Toxin

• AlphaFold DB: P77692

Antitoxin

• AlphaFold DB: Q47684