Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 418305..418802 Replicon chromosome
Accession NZ_CP044410
Organism Escherichia coli strain ecMN1F

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag FNW43_RS01910 Protein ID WP_001173343.1
Coordinates 418629..418802 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag FNW43_RS01905 Protein ID WP_000398619.1
Coordinates 418305..418601 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FNW43_RS01885 415036..415755 - 720 WP_000611288.1 two-component system response regulator DcuR -
FNW43_RS01890 415752..417383 - 1632 WP_001216477.1 sensor histidine kinase -
FNW43_RS01895 417564..417794 + 231 WP_000371704.1 (4Fe-4S)-binding protein -
FNW43_RS01900 417806..418078 + 273 WP_000405651.1 N-acetyltransferase -
FNW43_RS01905 418305..418601 + 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
FNW43_RS01910 418629..418802 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
FNW43_RS01915 418921..420438 - 1518 WP_001295074.1 lysine--tRNA ligase -
FNW43_RS01920 420675..422132 - 1458 WP_000856829.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T140870 WP_001173343.1 NZ_CP044410:418629-418802 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T140870 NZ_CP059488:c1783538-1783242 [Acidithiobacillus ferrivorans]
ATGAGTGAAAAATTGTCTACCTTTGACCCTGCCGAAGGGCTGACATCAGACGCGGCCATCGCAGCCTTTATGGCCGATGC
GTTTGAATCTGCAGACGCCGGCTACATTGCGCACGCGCTGGGCGTCGTGGCCCGCGCCAAGGGAATGGCTCAGATCGCTG
GAGAAACTGGTCTTTCGCGTGAACAGTTGTACCGCTCATTCAGCGAGCGCGGCAATCCGACGCTCAAGACCACGCTCGCA
GTCATGAAGGTCCTGGGGATTGAACTTACGGCAAAACCGGGCCCACAGGGCCGATGA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT140870 WP_000398619.1 NZ_CP044410:418305-418601 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT140870 NZ_CP059488:c1783837-1783541 [Acidithiobacillus ferrivorans]
ATGACCGAGCTCAAGCAAACAGAGACCTTCAGAAGGTGGTGGACGAAGCTCAAAGATGAACGAGCTCGCGGTATCGTTGC
CGGTCGTCTGGATCGCCTCGCTTATGGGTTGATCGGCGACGCGGAGCCAGTGGGAGAGGGTATAAGTGAGTTACGCATCC
ACTATGGCCCCGGGTACCGCGTTTATTTTCAGCAACGAGGGAGCATGATCATCGTCTTGCTCTGTGGTGGCAACAAGAAC
ACGCAAGCGAAGGACATCAAGGTGGCGAAGCGCCTGGCTGCTGAATGGAGAGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References