Detailed information of TA system TA140866

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 15901..16493
• Replicon: plasmid pNMBU-W10C18_04
• Accession: NZ_CP044406
• Organism: Escherichia coli strain NMBU-W10C18

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: J7LNC2
• Locus tag: F7O57_RS26025
• Protein ID: WP_000520550.1
• Coordinates: 16245..16493 (-)
• Length: 83 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: V0AWG0
• Locus tag: F7O57_RS26020
• Protein ID: WP_000681613.1
• Coordinates: 15901..16248 (-)
• Length: 116 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F7O57_RS25990	11901..12173	-	273	WP_001329503.1	TrbC/VirB2 family protein	-
F7O57_RS25995	12241..12861	-	621	WP_000157548.1	lytic transglycosylase domain-containing protein	-
F7O57_RS26000	13221..13454	-	234	WP_000120384.1	EexN family lipoprotein	-
F7O57_RS26005	13459..13725	-	267	WP_000134734.1	hypothetical protein	-
F7O57_RS26010	13802..15031	-	1230	WP_001329504.1	relaxase/mobilization nuclease domain-containing protein	-
F7O57_RS26015	15035..15538	-	504	WP_000781817.1	hypothetical protein	-
F7O57_RS26020	15901..16248	-	348	WP_000681613.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
F7O57_RS26025	16245..16493	-	249	WP_000520550.1	type II toxin-antitoxin system HicA family toxin	Toxin
F7O57_RS26030	16798..17148	-	351	WP_000854257.1	hypothetical protein	-
F7O57_RS26035	17242..17541	-	300	WP_000650312.1	hypothetical protein	-
F7O57_RS26040	17995..18222	-	228	WP_000801440.1	hypothetical protein	-
F7O57_RS26045	18212..18460	-	249	WP_001230706.1	hypothetical protein	-
F7O57_RS26050	18457..18999	-	543	WP_001083907.1	hypothetical protein	-
F7O57_RS26055	19104..19619	-	516	WP_001025395.1	J domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..32448	32448

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (20-81)

Antitoxin

• Uncharacterised protein family HicB (54-104)

Sequences

Toxin

Protein

• Length: 83 a.a.
• Molecular weight: 9437.05 Da
• Isoelectric Point: 10.4800

>T140866 WP_000520550.1 NZ_CP044406:c16493-16245 [Escherichia coli]
MGKTDKLQAKFLNSKKTFEWDELVVLFSSLGYVKKEMQGSRVRFFNAEINHTILMHRPHPESYIKGGTLKAIKQNLKEAG
LL

Nucleotide

• Length: 249 bp

>T140866 NZ_CP059488:c1049237-1048833 [Acidithiobacillus ferrivorans]
ATGGCGCGATACATGCTCGACACCAATATGTGCATTTACCTGATGAAGAACCAGCCAGAAGAAGTCGCCAAGCGTTTTGC
ACGGTGCTACGTCGGCGATGTCGTGATGTCCTCAATCACCTACGCCGAACTGACATACGGTGTGGCCGCCGCAAACGATC
CGGAACAAGAACGCATCAACCTCGCCAGCCTGGTGGATGATATACAGGTGGCTCCGTTTGATATTGCTGCCGGTGTATCC
TACGGTCCCATCCGGTTAGCTACCCGTGACACCAGGACAAATGCGCTCGACAAGCTGATTGCCGCACATGCTATATCGCT
GAACGTGATTATCGTGACCAACAACACAAAGGACTTCATCAAATATCCAGGGGCGATTTTGGAAAATTGGCTTCTCGAAA
CATAA

Antitoxin

Protein

• Length: 116 a.a.
• Molecular weight: 13113.84 Da
• Isoelectric Point: 4.6723

>AT140866 WP_000681613.1 NZ_CP044406:c16248-15901 [Escherichia coli]
MKHLKYKGYLGTVEPDFENNVLYGKLAFIRDLVTYEASTLAELEQEFKTSVDLYLQSCVEDGKEPDTPFKGVFNVRLDPE
LHRRVAEMAMEEDLSLNAFVNKALEKEVSNHRAGA

Nucleotide

• Length: 348 bp

>AT140866 NZ_CP059488:c1049473-1049237 [Acidithiobacillus ferrivorans]
ATGCATACCACCCGAATTTTCAAGAACGGGAACTCACAGGCCGTCCGTATCCCGGCGGATATCGCCTATGAGCGCACCGA
CATGGATTTGGAGATTGAGCGTGATGGCGATGAAATCCGGATACGACCCGCGCGTCGGTCTTTGTCTATGGTGCTGACCA
AGTTTGCCAGGTTCGCCCCGGACTTCATGGCTGAGGGTCGCGGCGACCAGCAACAGGTAGAGAGAGACCCTCTGTAA

Structures

Toxin

• AlphaFold DB: A0A6Y5WGJ7

Antitoxin

• AlphaFold DB: A0A829CLC1