Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 15901..16493 Replicon plasmid pNMBU-W10C18_04
Accession NZ_CP044406
Organism Escherichia coli strain NMBU-W10C18

Toxin (Protein)


Gene name hicA Uniprot ID J7LNC2
Locus tag F7O57_RS26025 Protein ID WP_000520550.1
Coordinates 16245..16493 (-) Length 83 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID V0AWG0
Locus tag F7O57_RS26020 Protein ID WP_000681613.1
Coordinates 15901..16248 (-) Length 116 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
F7O57_RS25990 11901..12173 - 273 WP_001329503.1 TrbC/VirB2 family protein -
F7O57_RS25995 12241..12861 - 621 WP_000157548.1 lytic transglycosylase domain-containing protein -
F7O57_RS26000 13221..13454 - 234 WP_000120384.1 EexN family lipoprotein -
F7O57_RS26005 13459..13725 - 267 WP_000134734.1 hypothetical protein -
F7O57_RS26010 13802..15031 - 1230 WP_001329504.1 relaxase/mobilization nuclease domain-containing protein -
F7O57_RS26015 15035..15538 - 504 WP_000781817.1 hypothetical protein -
F7O57_RS26020 15901..16248 - 348 WP_000681613.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
F7O57_RS26025 16245..16493 - 249 WP_000520550.1 type II toxin-antitoxin system HicA family toxin Toxin
F7O57_RS26030 16798..17148 - 351 WP_000854257.1 hypothetical protein -
F7O57_RS26035 17242..17541 - 300 WP_000650312.1 hypothetical protein -
F7O57_RS26040 17995..18222 - 228 WP_000801440.1 hypothetical protein -
F7O57_RS26045 18212..18460 - 249 WP_001230706.1 hypothetical protein -
F7O57_RS26050 18457..18999 - 543 WP_001083907.1 hypothetical protein -
F7O57_RS26055 19104..19619 - 516 WP_001025395.1 J domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..32448 32448


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(20-81)

Antitoxin

Uncharacterised protein family HicB

(54-104)


Sequences

Toxin        


Download         Length: 83 a.a.        Molecular weight: 9437.05 Da        Isoelectric Point: 10.4800

>T140866 WP_000520550.1 NZ_CP044406:c16493-16245 [Escherichia coli]
MGKTDKLQAKFLNSKKTFEWDELVVLFSSLGYVKKEMQGSRVRFFNAEINHTILMHRPHPESYIKGGTLKAIKQNLKEAG
LL

Download         Length: 249 bp

>T140866 NZ_CP059488:c1049237-1048833 [Acidithiobacillus ferrivorans]
ATGGCGCGATACATGCTCGACACCAATATGTGCATTTACCTGATGAAGAACCAGCCAGAAGAAGTCGCCAAGCGTTTTGC
ACGGTGCTACGTCGGCGATGTCGTGATGTCCTCAATCACCTACGCCGAACTGACATACGGTGTGGCCGCCGCAAACGATC
CGGAACAAGAACGCATCAACCTCGCCAGCCTGGTGGATGATATACAGGTGGCTCCGTTTGATATTGCTGCCGGTGTATCC
TACGGTCCCATCCGGTTAGCTACCCGTGACACCAGGACAAATGCGCTCGACAAGCTGATTGCCGCACATGCTATATCGCT
GAACGTGATTATCGTGACCAACAACACAAAGGACTTCATCAAATATCCAGGGGCGATTTTGGAAAATTGGCTTCTCGAAA
CATAA

Antitoxin


Download         Length: 116 a.a.        Molecular weight: 13113.84 Da        Isoelectric Point: 4.6723

>AT140866 WP_000681613.1 NZ_CP044406:c16248-15901 [Escherichia coli]
MKHLKYKGYLGTVEPDFENNVLYGKLAFIRDLVTYEASTLAELEQEFKTSVDLYLQSCVEDGKEPDTPFKGVFNVRLDPE
LHRRVAEMAMEEDLSLNAFVNKALEKEVSNHRAGA

Download         Length: 348 bp

>AT140866 NZ_CP059488:c1049473-1049237 [Acidithiobacillus ferrivorans]
ATGCATACCACCCGAATTTTCAAGAACGGGAACTCACAGGCCGTCCGTATCCCGGCGGATATCGCCTATGAGCGCACCGA
CATGGATTTGGAGATTGAGCGTGATGGCGATGAAATCCGGATACGACCCGCGCGTCGGTCTTTGTCTATGGTGCTGACCA
AGTTTGCCAGGTTCGCCCCGGACTTCATGGCTGAGGGTCGCGGCGACCAGCAACAGGTAGAGAGAGACCCTCTGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A6Y5WGJ7

Antitoxin

Source ID Structure
AlphaFold DB A0A829CLC1

References