140855

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 4060549..4061132 Replicon chromosome
Accession NZ_CP044403
Organism Escherichia coli strain NMBU-W10C18

Toxin (Protein)


Gene name mazF Uniprot ID S1EZP4
Locus tag F7O57_RS19975 Protein ID WP_000254738.1
Coordinates 4060549..4060884 (-) Length 112 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID S1P3W5
Locus tag F7O57_RS19980 Protein ID WP_000581937.1
Coordinates 4060884..4061132 (-) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
F7O57_RS19960 4056436..4057734 - 1299 WP_000036723.1 phosphopyruvate hydratase -
F7O57_RS19965 4057821..4059458 - 1638 WP_000210878.1 CTP synthase (glutamine hydrolyzing) -
F7O57_RS19970 4059686..4060477 - 792 WP_001071643.1 nucleoside triphosphate pyrophosphohydrolase -
F7O57_RS19975 4060549..4060884 - 336 WP_000254738.1 endoribonuclease MazF Toxin
F7O57_RS19980 4060884..4061132 - 249 WP_000581937.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
F7O57_RS19985 4061210..4063444 - 2235 WP_000226798.1 GTP diphosphokinase -
F7O57_RS19990 4063492..4064049 - 558 Protein_3832 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -
F7O57_RS19995 4064093..4065052 + 960 Protein_3833 IS1380-like element ISEc9 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 4055504..4066521 11017


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-109)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 12098.04 Da        Isoelectric Point: 8.2618

>T140855 WP_000254738.1 NZ_CP044403:c4060884-4060549 [Escherichia coli]
MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Download         Length: 336 bp

>T140855 NZ_CP059481:c4932408-4932094 [Enterobacter kobei]
ATGCATTTAATCTCAATGAAGGCAATTTTGGATGCGGTGATCCAATTTCCGCAACACAGAGAGGAGCTTCTCTTTTTGGG
GCGAACCATCGAAAAGAGCCATTGCACGACGCCTGTTGCGCTAAGAAAAATCTTTCCAACGCTAGATAATTTGAAGTATC
TCGATAAGCATTATGTTATTGATATTGCTAACAACAACCTCAGAGTAGTGGCTCTCATATTCTTTGAAAGCCAAAAGTTT
TATGTGCGCCATGTTTTTACTCATAAGGAATATGACCGTTTTACGGAGAAACATCGCACTAAGGGGAAGAAATGA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9355.74 Da        Isoelectric Point: 4.4665

>AT140855 WP_000581937.1 NZ_CP044403:c4061132-4060884 [Escherichia coli]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Download         Length: 249 bp

>AT140855 NZ_CP059481:c4932097-4931678 [Enterobacter kobei]
ATGATGATCGTAGCCGATGCAATGAAAGCAACGCATGCCCTTGTCGCGGCGGTTCCTTTATTGGGTGAAAATCCAAACGA
GAAAGATTACCAGGACGCACTTGAGCTGGTTGAGTATTTACTGATGAACGAACCTGGCAGCCCTTTGCTGGATATCGTAT
GTGCAAGAATTCGTCGTTACGAAGCCAATCGGCCCGAAATTGTCGCCTTACGCCAGGAGATGGAATCTGTCCCAGTGGGC
ATTGCCGTTTTAAGAACCCTTATGGATCAATACAAACTGACTATTTCGGATTTTCAGGATGAAATTGGCAGTAAATCAAT
GGTTTCAAGGGTGTTGAACGGTCAACGTCAGCTGACGTTGAACCATATAAAAAAACTGGCTGCTCGCTTTGGGGTATCGC
CTGCGCTGTTCATTGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 1UB4
PDB 5CQX
PDB 5CK9
PDB 5CKH
PDB 5CO7
PDB 3NFC
PDB 5CR2
PDB 5CKB
PDB 5CQY
PDB 5CKD
PDB 5CKE
PDB 5CKF

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LMB4

References