Detailed information of TA system TA140843

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2554428..2555066
• Replicon: chromosome
• Accession: NZ_CP044403
• Organism: Escherichia coli strain NMBU-W10C18

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A0D8WF76
• Locus tag: F7O57_RS12575
• Protein ID: WP_001306887.1
• Coordinates: 2554890..2555066 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: F7O57_RS12570
• Protein ID: WP_001270286.1
• Coordinates: 2554428..2554844 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F7O57_RS12550	2549580..2550521	-	942	WP_001251315.1	ABC transporter permease	-
F7O57_RS12555	2550522..2551535	-	1014	WP_001523231.1	ABC transporter ATP-binding protein	-
F7O57_RS12560	2551553..2552698	-	1146	WP_020233879.1	ABC transporter substrate-binding protein	-
F7O57_RS12565	2552943..2554349	-	1407	WP_001523229.1	PLP-dependent aminotransferase family protein	-
F7O57_RS12570	2554428..2554844	-	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
F7O57_RS12575	2554890..2555066	-	177	WP_001306887.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
F7O57_RS12580	2555288..2555518	+	231	WP_000494241.1	YncJ family protein	-
F7O57_RS12585	2555610..2557571	-	1962	WP_024166512.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
F7O57_RS12590	2557644..2558180	-	537	WP_020233877.1	DNA-binding transcriptional regulator SutR	-
F7O57_RS12595	2558233..2559444	+	1212	WP_023143671.1	BenE family transporter YdcO	-
F7O57_RS12600	2559552..2560010	-	459	WP_000526135.1	IS200/IS605 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	2559552..2560010	458

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6768.88 Da
• Isoelectric Point: 11.5336

>T140843 WP_001306887.1 NZ_CP044403:c2555066-2554890 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFRGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T140843 NZ_CP059478:c102533-102240 [Acinetobacter baumannii]
ATGACTTATAAGCTTTTACGTCACAAGGACTTTACAGCAGAATGGGAAAAACTCCCTGTTGCTATACGAGATCAATTTAA
AAAGAAACTAGCCAAAGTCATAGAGCAGCCACATATTCCAAAGAATATGCTTAGAGGCGATCTTGCAGGTTGCTATAAAA
TCAAATTATTAAAAGCTGGTGTCCGACTTGTCTATCAAGTTAAAGATGATCAGGTAGTTATCCTGCTCATTACCGTCGGA
AAACGAGCTGATAGCATAGTTTATGACGAAGCGAAAAAGCGAATTAAAGACTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT140843 WP_001270286.1 NZ_CP044403:c2554844-2554428 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT140843 NZ_CP059478:c102768-102523 [Acinetobacter baumannii]
ATGGCTACTGTTAACTTCAGGATCGATGAAGCCCTTAAAGAAAAATCTTATTCAATCCTTAAAGAGCAAGGTATTGCACC
TACTGATTTCTTCACTAGTATTCTTGAGTATGTTGCTACGACTGGGAAACTGCCTGTCAAAAAAGCTTTGCTTTCGGAAG
AAGATGAAGAATTATTAGCTCTTGTCCGTAAGCGCATTAATGATCCTAAAGAGATGTTTGAGGAAGTCACATTAGATGAC
TTATAA

Structures

Toxin

• AlphaFold DB: A0A0D8WF76