Detailed information of TA system TA140842

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 2452481..2453442
• Replicon: chromosome
• Accession: NZ_CP044403
• Organism: Escherichia coli strain NMBU-W10C18

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: A0A1Q5ZNB7
• Locus tag: F7O57_RS12135
• Protein ID: WP_021523084.1
• Coordinates: 2452747..2453442 (+)
• Length: 232 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: F7O57_RS12130
• Protein ID: WP_001296726.1
• Coordinates: 2452481..2452747 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F7O57_RS12130	2452481..2452747	+	267	WP_001296726.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
F7O57_RS12135	2452747..2453442	+	696	WP_021523084.1	HipA N-terminal domain-containing protein	Toxin
F7O57_RS12140	2453642..2454994	-	1353	WP_001551127.1	SIR2 family protein	-
F7O57_RS12145	2455209..2456241	-	1033	Protein_2294	ISNCY family transposase	-
F7O57_RS12150	2456608..2457507	-	900	WP_001523294.1	LysR family transcriptional regulator	-
F7O57_RS12155	2457646..2457954	+	309	WP_001221046.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	2455209..2456027	818

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (4-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-69)

Sequences

Toxin

Protein

• Length: 232 a.a.
• Molecular weight: 25773.79 Da
• Isoelectric Point: 9.9928

>T140842 WP_021523084.1 NZ_CP044403:2452747-2453442 [Escherichia coli]
MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLANRYARPLSLSLPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYH
AKSRQPFDLLSEIGRDSVGAVTLIPEDETIQAGGSYRLTPFYDIISAFPVLGGAGIHISDLKLAMGVNASKGKKTAIDKI
YPRHFLATAKVLKFPEVQMHEILSDFARMIPAALDNVKTSLPTDFPENVVTAVETNVLRLHGRLSREYGIK

Nucleotide

• Length: 696 bp

>T140842 NZ_CP059478:21300-21620 [Acinetobacter baumannii]
ATGTTATTTATTGAAACCAGCATCTTTACCAAGCAAATTAAAGACCTTGTATCTGATGAGGAATATCGTCAACTTCAGCA
AGATCTTTTGGTACAGCCTGATAGAGGTGACCTAATCAAGAATGGCGGTGGTATTCGCAAAGTACGTTGTGCTCAAGGTA
ACAAAGGGAAAAGTGGTGGGATACGGGTGATTTATTATTGGGTCACCGAGGATGATCAGATCTTTTTCTTAGTGGCTTAT
CCAAAATCAGTAAAAGATAATTTAACAGATAAGGAAACCGCCATTCTGCACCAACTCGTGAAGGAGCAATTTCATGGATA
A

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10047.39 Da
• Isoelectric Point: 4.9632

>AT140842 WP_001296726.1 NZ_CP044403:2452481-2452747 [Escherichia coli]
MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISNFENNPDNTTLTTFFKILQSLELSMTLCDTKNASPES
TEQQDLEW

Nucleotide

• Length: 267 bp

>AT140842 NZ_CP059478:21613-21885 [Acinetobacter baumannii]
ATGGATAACAACTTATTTGATGACTTGGTTGCTTCAATCAAAGAAGCTGGAGCTATCAAACGTAAAGAAGTTAAAGCAAG
CCGAGTTACAGAACTCGAATTGCCTGATATTAAAGAAGTACGTGAAAAAACTGGCTTGAGCCAAAATGAATTTGCTGCAC
GTCTACATATTAGCCCCCGTACCCTGCAAAATTGGGAACAGGGACGACGCTATCCAACTGGACCAGCAGCTACATTGATT
CGAATTTTAGATGCTCACCCAAGCCTTATTTGA

Structures

Toxin

• AlphaFold DB: A0A1Q5ZNB7