Detailed information of TA system TA140829

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yjhX-yjhQ/YjhX-GNAT
• Location: 724124..724938
• Replicon: chromosome
• Accession: NZ_CP044403
• Organism: Escherichia coli strain NMBU-W10C18

Toxin (Protein)

• Gene name: yjhX
• Uniprot ID: S1PA82
• Locus tag: F7O57_RS03800
• Protein ID: WP_001054376.1
• Coordinates: 724681..724938 (-)
• Length: 86 a.a.

Antitoxin (Protein)

• Gene name: yjhQ
• Uniprot ID: U9Z4B8
• Locus tag: F7O57_RS03795
• Protein ID: WP_001309181.1
• Coordinates: 724124..724669 (-)
• Length: 182 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F7O57_RS03770	719815..721128	-	1314	WP_000460843.1	galactitol-specific PTS transporter subunit IIC	-
F7O57_RS03775	721140..721418	-	279	WP_000722973.1	PTS sugar transporter subunit IIB	-
F7O57_RS03780	721415..722536	-	1122	WP_172986673.1	M42 family metallopeptidase	-
F7O57_RS03785	722935..723153	-	219	Protein_658	hypothetical protein	-
F7O57_RS03790	723322..724068	-	747	WP_000354251.1	class I SAM-dependent methyltransferase	-
F7O57_RS03795	724124..724669	-	546	WP_001309181.1	N-acetyltransferase	Antitoxin
F7O57_RS03800	724681..724938	-	258	WP_001054376.1	hypothetical protein	Toxin
F7O57_RS03805	725315..725560	-	246	Protein_662	GNAT family N-acetyltransferase	-
F7O57_RS03810	725930..726916	+	987	WP_001323209.1	hypothetical protein	-
F7O57_RS03815	727499..728479	-	981	WP_000991438.1	9-O-acetyl-N-acetylneuraminic acid deacetylase	-
F7O57_RS03820	728544..729650	-	1107	WP_001309184.1	N-acetylneuraminate epimerase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Putative toxin of bacterial toxin-antitoxin pair (1-85)

Antitoxin

• GNAT domain (18-121)

Sequences

Toxin

Protein

• Length: 86 a.a.
• Molecular weight: 9734.29 Da
• Isoelectric Point: 11.0090

>T140829 WP_001054376.1 NZ_CP044403:c724938-724681 [Escherichia coli]
MNLSRQEQHTLHVLAKGRRIAHVRDSSGRVTSVECYSREGLLLTDCTLAVFKKLKTKKLIKSVNGQPYRINTTELNKVRA
QLDNR

Nucleotide

• Length: 258 bp

>T140829 NZ_CP059468:c4068952-4068850 [Klebsiella pneumoniae]
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 182 a.a.
• Molecular weight: 19956.90 Da
• Isoelectric Point: 6.3277

>AT140829 WP_001309181.1 NZ_CP044403:c724669-724124 [Escherichia coli]
MTVHHFTFHITDKSDASDIREVETRAFGFSKEADLVASLLEDESARPALSLLARYEGKAVGHILFTRATFKGEMDSPLMH
ILAPLAVIPEYQGMGVGGRLIRTGIEHLRLMGCQTVFVLGHATYYPRHGFEPCAGDKGYPAPYPIPEEHKACWMMQSLTA
QPMTLTGHIRCADPDETGALT

Nucleotide

• Length: 546 bp

>AT140829 NZ_CP059468:4068843-4068988 [Klebsiella pneumoniae]
TAAAGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAG
TTGGCATTAATGCAGGCTAAGTCGCCTTGCACTATAAGAATAGTTTAACGCGTCAGCTTTTCCAGT

Structures

Toxin

• AlphaFold DB: S1PA82

Antitoxin

• AlphaFold DB: U9Z4B8