Detailed information of TA system TA140819

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/RelE-RHH
• Location: 1987..2534
• Replicon: plasmid pNMBU-W10C18_02
• Accession: NZ_CP044402
• Organism: Escherichia coli strain NMBU-W10C18

Toxin (Protein)

• Gene name: parE
• Uniprot ID: E9LLH3
• Locus tag: F7O57_RS00020
• Protein ID: WP_000969996.1
• Coordinates: 2253..2534 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: F4V9S6
• Locus tag: F7O57_RS00015
• Protein ID: WP_000079931.1
• Coordinates: 1987..2256 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F7O57_RS00005	46..1077	+	1032	WP_106490007.1	replication initiation protein	-
F7O57_RS00010	1774..1914	+	141	WP_012512742.1	hypothetical protein	-
F7O57_RS00015	1987..2256	+	270	WP_000079931.1	hypothetical protein	Antitoxin
F7O57_RS00020	2253..2534	+	282	WP_000969996.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
F7O57_RS00025	2729..3070	-	342	WP_000578011.1	hypothetical protein	-
F7O57_RS00030	3370..3957	+	588	WP_106490006.1	serine acetyltransferase	-
F7O57_RS00035	4239..4832	-	594	WP_032274973.1	proQ/FINO family protein	-
F7O57_RS00040	5433..6023	-	591	WP_106490005.1	hypothetical protein	-
F7O57_RS00045	6023..6280	-	258	WP_000343099.1	hypothetical protein	-
F7O57_RS00050	6850..7263	+	414	WP_106490004.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	blaCMY-42	-	1..49118	49118

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-92)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10893.57 Da
• Isoelectric Point: 8.4615

>T140819 WP_000969996.1 NZ_CP044402:2253-2534 [Escherichia coli]
MMEIFWTMLASQDRKRIREYIAEQNLMAAIELDERIGYSASSLAGQPYKGRNGRVEGTRELVIHPHFVLVYEVDSQWGKV
YILRVLHTAQKWP

Nucleotide

• Length: 282 bp

>T140819 NZ_CP059467:3796656-3797408 [Aromatoleum bremense]
GTGACGGACATCTGGGCGGCGGTCGGTGGAGAGGCCCCGGTCGTCGAGCTCGCCGGCAATCTCGTGCGGCTCGTCGAGAG
CCAGGAGCAGATCGCGACGAACCGCCTCGTCGATACGCTCGAGGAACAGGCGCTCCTCGAATCCCTGCTGGAAAGCTCGA
AGCCGCCGCTGCCGCAAGCGGCCCGCGGCCTGCATTACCTGCTGGCGACCCCGTTCCGTTATCCGCCGCTGCGCCACGGC
TCGCGTTTCGGCGGGCACTTCGAGCCCAGCCTGTTCTACGCGGCGTTCGATACGGCAACGGTGCTGGTCGAATCGGCCTA
TTACCGCTTCGTCTTCTGGCATGGCATGGCCGTCGCGCCGGCCTCGTCCCTGGCGTCCCAGCACACGCTGTTCGGCGCGC
CGTGGCGCTGCGCCCGCGGCGTGCAACTGCAGTTGCCGCCGTTCGACCACTGGCGCGAGCAACTCACCGACCCCGCGGAT
TACGCCGCGACCCAGCGACTCGGCACGGCGATGCGGAAAGCCGGCGTCGAAGCTTTCGAATACGTCTCGGCGCGCGACCC
CGCCGGCGGCCTCAACGTCGCGCTATTCACCCCGATGGCGCTGGCCGCCCGGCGGCCGGATTTCATGCAGGCGTGGCTCG
CGGAGACGGATGCGGCCGAGGTGCGCTTCTACTGCCAGGAAGAGCGGCTCGTGCATCGCTTCCCGCTCGGGCAGTTCCTC
GTCGCGGGCGGGCTGCCGATGCCGGCGGTATAG

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10194.55 Da
• Isoelectric Point: 8.8735

>AT140819 WP_000079931.1 NZ_CP044402:1987-2256 [Escherichia coli]
MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQHNTWFRDQVAAGRQQLERGDVLPHDMVESSAAAWRD
EMSRKVAGK

Nucleotide

• Length: 270 bp

>AT140819 NZ_CP059467:3796300-3796659 [Aromatoleum bremense]
ATGAGCGTTGCTGCCCTTCGCAAACCCGATGCGACGCAGGTCGTTACCAAGGCCCTGCTCAACGCCGGCAAGGAAATGGG
GCTGACGCAGGCGGCGCTGGGCGTGGTCATCGGCAAGGACCGGACCGCGATCAGCCGCAACGGCATCGACCCCGACAGCA
AGGCCGGAGAGCTCGCGCTGCTGCTGATCCGCGCGTATCGCGCGCTGTTTGCGCTCGTCGGGGGCGACGCCGCGCAGATG
AAGCACTGGATGCACACCGACAACCACCACGTCGGCGGCGTCCCGGCCGAGCAGATTCGCACCGTGCAGGGCTTGCTGCG
CGTCGTGGAATACCTCGACGCGATCCGCGGCCGGCTGTGA

Structures

Toxin

• AlphaFold DB: E9LLH3

Antitoxin

• AlphaFold DB: A0A7U9DLR5