Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 2400670..2401321 Replicon chromosome
Accession NZ_CP044361
Organism Lacticaseibacillus paracasei strain TD 062

Toxin (Protein)


Gene name pemK Uniprot ID K6S8L5
Locus tag F5J09_RS12250 Protein ID WP_003567661.1
Coordinates 2400670..2401053 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID K6RKI5
Locus tag F5J09_RS12255 Protein ID WP_003567665.1
Coordinates 2401073..2401321 (-) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
F5J09_RS12245 2399965..2400486 - 522 WP_003599928.1 QueT transporter family protein -
F5J09_RS12250 2400670..2401053 - 384 WP_003567661.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
F5J09_RS12255 2401073..2401321 - 249 WP_003567665.1 antitoxin Antitoxin
F5J09_RS12260 2401389..2402525 - 1137 WP_003599931.1 alanine racemase -
F5J09_RS12265 2402512..2402886 - 375 WP_003567669.1 holo-ACP synthase -
F5J09_RS12270 2403056..2404564 - 1509 WP_003567670.1 DEAD/DEAH box helicase -
F5J09_RS14750 2404690..2404833 - 144 WP_003588978.1 hypothetical protein -
F5J09_RS12275 2404864..2406252 - 1389 WP_003567677.1 UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(6-112)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 13773.03 Da        Isoelectric Point: 10.9764

>T140813 WP_003567661.1 NZ_CP044361:c2401053-2400670 [Lacticaseibacillus paracasei]
MDVSVKRGDVFFADLSPVVGSEQGGNRPVLIIQNNVGNHYSPTVIVAAITSKIQKPKMPTHVGLRAKQDGVERNSVILLE
QIRTIDKQRLQARVTALSSTKMAAVDRALAISVGLVSLPKPKTYNKN

Download         Length: 384 bp

>T140813 NZ_CP059467:1657230-1657556 [Aromatoleum bremense]
ATGATGCGGGGCGACTTCGTGACCATCGCCATGCAAGGCGATTTTGGCAAGCCAGGACTCGCCCTGGTGATTCAAGCCGA
CCCGTTTAGCGAGCACGCCACCGTCACGGTGCTGCCGGTAACAAGCGCGCTGGTCGCTGCGCCGCTGTTTCGCATCACTG
TCCAACCCAGCGCAGAGAACGGCCTGCAGAAGCCTTCGCAGGTGATGGCGGACAAGGCCATCACCGTGAAACGCGCCAAG
ATAGGGCAAGCCTTCGGCCGTATTGATGCGGATGCGATGGTGGAGGTCGAGCGTTGCCTGGCCGTGTTCCTGGGGATTGC
CAAGTGA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 8938.08 Da        Isoelectric Point: 4.4610

>AT140813 WP_003567665.1 NZ_CP044361:c2401321-2401073 [Lacticaseibacillus paracasei]
MKKQTSVAVTFDDAIKARLDAYCELNDIDAQAVITEAVATFLKSQAPEINQLISGYVAMGQINTEISHAFSACESEAYAH
IR

Download         Length: 249 bp

>AT140813 NZ_CP059467:1657006-1657230 [Aromatoleum bremense]
ATGGCAAACACGCCCGTAAATGCGCGGGTTCAAAAGCACCGCGACGCGCTGCGCATGGCCGGGCTTCGCCCGGTGCAAAT
CTGGGTGCCGGATACGCGACGGCCAGACTTTGCAGAGGAATGCCGCCGCCAGAGCCGCCTTGCTGCCGAAGCGGACATGG
CCGACACGGACATGCAGCACTTCATGGATGAAGCCCTGGCCGACGTTGACGGCTGGACGGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB K6S8L5

Antitoxin

Source ID Structure
AlphaFold DB A0A0E2BNY2

References