Detailed information of TA system TA140811

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): spoIISABC/SpoIISA-SpoIISB
• Location: 1174977..1175894
• Replicon: chromosome
• Accession: NZ_CP044360
• Organism: Bacillus amyloliquefaciens strain V167

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: -
• Locus tag: BACIH_RS06260
• Protein ID: WP_012117368.1
• Coordinates: 1175148..1175894 (-)
• Length: 249 a.a.

Antitoxin (Protein)

• Gene name: spoIISB
• Uniprot ID: I2HQ14
• Locus tag: BACIH_RS06255
• Protein ID: WP_003154807.1
• Coordinates: 1174977..1175147 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BACIH_RS06210	1170111..1170623	+	513	WP_012117362.1	sigma-70 family RNA polymerase sigma factor	-
BACIH_RS06215	1170736..1170957	+	222	Protein_1175	terminase	-
BACIH_RS06220	1171366..1171833	+	468	WP_077411063.1	hypothetical protein	-
BACIH_RS06225	1171846..1172217	+	372	WP_012117365.1	hypothetical protein	-
BACIH_RS06230	1172222..1172419	+	198	WP_012117366.1	XkdX family protein	-
BACIH_RS06235	1172476..1173237	+	762	WP_012117367.1	hypothetical protein	-
BACIH_RS06240	1173289..1173552	+	264	WP_003154815.1	hemolysin XhlA family protein	-
BACIH_RS06245	1173566..1173829	+	264	WP_003154813.1	phage holin	-
BACIH_RS06250	1173843..1174721	+	879	WP_007407257.1	N-acetylmuramoyl-L-alanine amidase	-
BACIH_RS06255	1174977..1175147	-	171	WP_003154807.1	type II toxin-antitoxin system SpoIISB family antitoxin	Antitoxin
BACIH_RS06260	1175148..1175894	-	747	WP_012117368.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
BACIH_RS06265	1175999..1176997	-	999	WP_007407255.1	inorganic phosphate transporter	-
BACIH_RS06270	1177010..1177627	-	618	WP_003154804.1	DUF47 domain-containing protein	-
BACIH_RS06275	1177914..1179230	-	1317	WP_007610842.1	amino acid permease	-
BACIH_RS06280	1179553..1180503	+	951	WP_012117369.1	ring-cleaving dioxygenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (3-239)

Antitoxin

• Antitoxin SpoIISB (1-56)

Sequences

Toxin

Protein

• Length: 249 a.a.
• Molecular weight: 29119.61 Da
• Isoelectric Point: 4.7003

>T140811 WP_012117368.1 NZ_CP044360:c1175894-1175148 [Bacillus amyloliquefaciens]
MLLFFQIMVWTMAAALILYVYASWRYEAKVKEKMFAIRKTWYLLFVLGSMVYWTYDPESLFAEWRQYLIVAVCFALIDAF
IFLSAYIKKLAGNELETDTREILEENNEMLHSYLEKLKTYQYLLKNEPIHVYYGSTEAYAEGISRLLAAYAEKMNVTASL
CDYSAQADKDRLTEHMPDAADVQSRLNRKDVYYDQKGRLVLIPFTVQNRHYVIKLTSENLLTEFDYLLFTSLTSIYDLML
PIEEEGDG

Nucleotide

• Length: 747 bp

>T140811 NZ_CP059467:1331106-1331390 [Aromatoleum bremense]
ATGGGGATCGTCTGGACGGAAGAGGCGATCGGCGACCTCGAAGAGATCCTCGCGTACTACTACGCCGAAGCCGGTCCGGC
AACGGCGCAGGCGGTCCAGGAGCGCCTCGTCACCGAAATCGTGGCACTCACGACATTCCCCGAGAGGATCCGCACGAGCG
GCCGCGTCCCCGGCGCCCGGGAACTTGTGGTGCGGCGCCTCCCGTACGTGGTCTTCGTCAAAGTGGTCCCCGCCGGCGTC
GTGGTGCTCAACGTGGTACACACGGCGAGGAAGTTTCCAGAGTAA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6676.74 Da
• Isoelectric Point: 10.9273

>AT140811 WP_003154807.1 NZ_CP044360:c1175147-1174977 [Bacillus amyloliquefaciens]
MEQAFQNGQPEQRAKPFKMFKKRSMTSIASYQVSPHTARIFKENERLIDKYKQKKA

Nucleotide

• Length: 171 bp

>AT140811 NZ_CP059467:1331521-1331724 [Aromatoleum bremense]
ATGAGAACGACTGTCACGATCGATGATGATCTGTACGAAAAGGCCCTGGAAGTGGCCGATCCGGCTATGGACAGGGCCGA
TCTCTTCCGCGAGGCGATGAAAACTTTCGTGCGAGTACAAGCCGCCAAGCGCCTTGCTGCGTTGGGGGGCACCATGCCGG
AGATGCAGGATGTGCCGCGACGGCGCGGCGAGCCCTCGCAATGA

Structures

Antitoxin

• AlphaFold DB: I2HQ14