Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 36553..37135 Replicon plasmid pXF879-41
Accession NZ_CP044353
Organism Xylella fastidiosa strain ATCC 35879

Toxin (Protein)


Gene name shpA Uniprot ID A0A060HA48
Locus tag F7G16_RS11875 Protein ID WP_038229226.1
Coordinates 36845..37135 (-) Length 97 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A060HE88
Locus tag F7G16_RS11870 Protein ID WP_004091409.1
Coordinates 36553..36858 (-) Length 102 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
F7G16_RS11840 33102..33527 + 426 WP_004091423.1 antirestriction protein -
F7G16_RS11845 33795..34052 + 258 WP_038229233.1 hypothetical protein -
F7G16_RS11850 34246..34878 - 633 WP_004091419.1 YkgJ family cysteine cluster protein -
F7G16_RS11855 34999..35556 - 558 WP_004091417.1 recombinase family protein -
F7G16_RS11860 35746..36120 - 375 WP_010894485.1 DUF5615 family PIN-like protein -
F7G16_RS11865 36117..36347 - 231 WP_004091411.1 DUF433 domain-containing protein -
F7G16_RS11870 36553..36858 - 306 WP_004091409.1 BrnA antitoxin family protein Antitoxin
F7G16_RS11875 36845..37135 - 291 WP_038229226.1 BrnT family toxin Toxin
F7G16_RS11880 37312..37485 + 174 Protein_42 XRE family transcriptional regulator -
F7G16_RS11885 37596..37916 + 321 WP_004091397.1 type II toxin-antitoxin system RelE/ParE family toxin -
F7G16_RS11890 38046..38372 + 327 WP_004091396.1 DNA-binding protein -
F7G16_RS11895 38514..39440 - 927 WP_051606204.1 hypothetical protein -
F7G16_RS11900 39846..40262 - 417 WP_038232460.1 IS200/IS605 family transposase -
F7G16_RS11905 40285..41442 + 1158 WP_038232468.1 transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..41753 41753
- inside IScluster/Tn - - 34999..41442 6443


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-89)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(11-93)


Sequences

Toxin        


Download         Length: 97 a.a.        Molecular weight: 11304.62 Da        Isoelectric Point: 6.0917

>T140800 WP_038229226.1 NZ_CP044353:c37135-36845 [Xylella fastidiosa]
VERYFEWDDKKAESNFRKHGIRFDEAALAFDDPFAVSEQNRIENGEQRWQTIGMAGGCLLLLVAHTVHFEDDGIEVVRII
SARRVDRKERRRYEHG

Download         Length: 291 bp

>T140800 NZ_CP059445:c1212613-1211693 [Campylobacter fetus]
ATGCAAGAACAATATACGGAAATCAAAGATAGCAATATTATTAATAAACAAAACATAGATATTGCAGTAGGGCTTGGGCT
TGTAGATGATCTGAAGCCTTCTGAGTATTTTTATAAGGCTGTTGAAAAATCGCGAACATACAACGAACTTGAAGACAATG
TAAAGAAATATTATGATGGCAAAAAGCTAGATAAAAAAGAGGTCGGCGAAAAAGAGTGCGATATAGTTTCAGTAAATATC
GCCAAGTATTTAGAAAAAAGTGGCTTTACGCTATCTCCTGTTACGTTGAAGACTATACATAAAAATTTATTTTGGGATGC
CTTTCCACAAGGGCTTGAAAAATATGTTGGAGTTTTTAGGGATGTAAATATTTCAAAAAAAGAAGAAGTCTTGGGTGGCG
AAAAAAGCGTAGAGTATGGTAATTATGATGAGCTTGGCGATATGCTGGATTATGATTTTGATAGAGAAAAACGCAAAGAT
TACACCAAAATGACTAGGCAAGAACAGGCTTTAAATGTCGGCAAATTTGTAAGCGGGATATGGCAAATTCATCCGTTTCG
AGAGGGCAATACTAGGACAATTGCGGTTTTTACTATTAAGTATCTGCAAAGTAAAGGCTTTGAAGCAAACAACGATATCT
TTAAAGAAAACTCAAAATATTTTAGGGATGCTTTAGTGTTAGCAAATTACGACAATATGAAAGAAAATATAAAAAGTGAT
TTTTCATACCTAGAGAGTTTTTTTAACAAATTTATTCTAAATAAAAATATAGAGTTAAAACTGCTACCAAACAGCCCAAA
AGATTACAAAAGTGAGAAATCGGTCCTATCTAGACTTGGTGCAGTCTATGACAAAAAGGTGCAAAGTGGAGCGATCAAAC
AACCACAAATAAGCGAAAAATCAAAAGAAAAGGAAAGATGA

Antitoxin


Download         Length: 102 a.a.        Molecular weight: 11684.43 Da        Isoelectric Point: 9.4870

>AT140800 WP_004091409.1 NZ_CP044353:c36858-36553 [Xylella fastidiosa]
MNMVRYKQSELPPLTEERKAELKALAKKPDSEIDYSDIPPLDESFWARAVPNPFFKPVKTHASVRIDSDVLAWLKSQGKG
YQTRMNAILREAMLRSLHQQE

Download         Length: 306 bp

>AT140800 NZ_CP059445:c1213449-1212613 [Campylobacter fetus]
ATGGATGGTGGCGAAAATTTAGGCAATTTCTTAAAAAACAACAACGAGCGAGACTATTGGCTAGATGTATGTGTTCGTAT
GGCTCATCATAGCACAGCAATCGAGGGTAATACTTTAAGTCAGGACGAGACTGCTTCAATCTTGCTTGATGGCTATATAG
CAAAAGCAACTAGCGAGCGTGAATTTTATGAAGTAAAAAACTATCGTCAAATTTTGCCAAAAATGTTTTCATCTTTACAA
GAAAAAGCCAAGATAGACGAGAAGCTAATAAAAGATTTTCATAGGCTAATAATGAATAATTTAATAGACAACAACGGCAA
ATTTAAAACTATCGAAAATTTAGTTGTAGGCGCAAATTTCGAACCTACAAAGCCGTATCTAGTGCCAGTATCAATTAAAG
ATATGTGCGATAATCTATATTTTAGACTTGATAATGCCAAAAATAATGACGATAAGCTAAAAGCAATATTGCAAAGCCAT
ATCAAATTTGAAAAAATTCATCCTTTTAGCGACGGAAACGGCAGAACCGGCAGGATAATTATGATCTATGGTTGTTTAGA
GAATAATTTAGCACCAATTATAATACCAAAAGAGCAAAAAAATAGATACATTGCAATACTGCGCAACAATGACATCGATG
GATTTATGGCGTTTGCTAAAGAAATAGAAAAAGATGAAATATCAAGGCGTAATAAATTTTTAAGCCAACGACCAAATGAG
CAAGATAATAGTAAATCAGTCTTATCTAGGCTCAGCAATGCTTACGCCAAAAAGCTAGAAAGCGGAGCGATCAAAGCATT
AAATGATACCGCAAATTCAAAGGAAAAGGAGAGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A060HA48

Antitoxin

Source ID Structure
AlphaFold DB A0A060HE88

References