Detailed information of TA system TA140790

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): spoIISABC/SpoIISA-SpoIISB
• Location: 1241296..1242213
• Replicon: chromosome
• Accession: NZ_CP044349
• Organism: Bacillus velezensis strain LC1

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: I2HQ15
• Locus tag: F6467_RS06535
• Protein ID: WP_007407256.1
• Coordinates: 1241467..1242213 (-)
• Length: 249 a.a.

Antitoxin (Protein)

• Gene name: spoIISB
• Uniprot ID: I2HQ14
• Locus tag: F6467_RS06530
• Protein ID: WP_003154807.1
• Coordinates: 1241296..1241466 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F6467_RS06495	1236529..1238151	+	1623	WP_032874601.1	pyocin knob domain-containing protein	-
F6467_RS06500	1238164..1238535	+	372	WP_032874603.1	YomQ/XkdW protein, phage-like element PBSX	-
F6467_RS06505	1238540..1238737	+	198	WP_032874605.1	XkdX family protein	-
F6467_RS06510	1238794..1239555	+	762	WP_032874607.1	hypothetical protein	-
F6467_RS06515	1239607..1239870	+	264	WP_003154815.1	hemolysin XhlA family protein	-
F6467_RS06520	1239884..1240147	+	264	WP_003154813.1	phage holin	-
F6467_RS06525	1240161..1241039	+	879	WP_032874609.1	N-acetylmuramoyl-L-alanine amidase	-
F6467_RS06530	1241296..1241466	-	171	WP_003154807.1	type II toxin-antitoxin system SpoIISB family antitoxin	Antitoxin
F6467_RS06535	1241467..1242213	-	747	WP_007407256.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
F6467_RS06540	1242318..1243316	-	999	WP_032874611.1	inorganic phosphate transporter	-
F6467_RS06545	1243329..1243946	-	618	WP_032874614.1	DUF47 domain-containing protein	-
F6467_RS06550	1244232..1245548	-	1317	WP_032874616.1	amino acid permease	-
F6467_RS06555	1245870..1246820	+	951	WP_032874618.1	ring-cleaving dioxygenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1197542..1300065	102523

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (3-239)

Antitoxin

• Antitoxin SpoIISB (1-56)

Sequences

Toxin

Protein

• Length: 249 a.a.
• Molecular weight: 29077.57 Da
• Isoelectric Point: 4.7755

>T140790 WP_007407256.1 NZ_CP044349:c1242213-1241467 [Bacillus velezensis]
MLLFFQIMVWTMAAALILYVYASWRYEAKVKEKMFAIRKTWYLLFVLGSMVYWTYDPESLFAAWRQYLIVAVCFALIDAF
IFLSAYIKKLAGNELETDTREILEENNEMLHSYLEKLKTYQYLLKNEPIHVYYGSTEAYAEGISRLLAAYAEKMNVTASL
CDYSAQSDKDRLTEHMPDAADVQSRLNRKDVYYDQKGRLVLIPFTVQNRHYVIKLTSENLLTEFDYLLFTSLTSIYDLML
PIEEEGDG

Nucleotide

• Length: 747 bp

>T140790 NZ_CP059425:c217500-217213 [Enterobacter hormaechei]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6676.74 Da
• Isoelectric Point: 10.9273

>AT140790 WP_003154807.1 NZ_CP044349:c1241466-1241296 [Bacillus velezensis]
MEQAFQNGQPEQRAKPFKMFKKRSMTSIASYQVSPHTARIFKENERLIDKYKQKKA

Nucleotide

• Length: 171 bp

>AT140790 NZ_CP059425:c217739-217500 [Enterobacter hormaechei]
ATGGGTAGCATTAACCTACGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGCGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGACTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Structures

Toxin

• AlphaFold DB: I2HQ15

Antitoxin

• AlphaFold DB: I2HQ14