Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 4345369..4346024 Replicon chromosome
Accession NZ_CP044345
Organism Mycobacterium tuberculosis strain L

Toxin (Protein)


Gene name vapC Uniprot ID P9WFA6
Locus tag F6W99_RS21210 Protein ID WP_003898861.1
Coordinates 4345369..4345770 (-) Length 134 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID G0TIK2
Locus tag F6W99_RS21215 Protein ID WP_003407272.1
Coordinates 4345767..4346024 (-) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
F6W99_RS21195 4340841..4342226 - 1386 WP_003407260.1 cytochrome P450 -
F6W99_RS21200 4342304..4343338 + 1035 WP_003407264.1 AraC family transcriptional regulator -
F6W99_RS21205 4343384..4345114 - 1731 WP_031694300.1 PE family protein -
F6W99_RS21210 4345369..4345770 - 402 WP_003898861.1 type II toxin-antitoxin system VapC family toxin Toxin
F6W99_RS21215 4345767..4346024 - 258 WP_003407272.1 ribbon-helix-helix domain-containing protein Antitoxin
F6W99_RS21220 4346107..4347066 - 960 WP_003407276.1 alpha/beta hydrolase -
F6W99_RS21225 4347091..4348053 - 963 WP_003407279.1 alpha/beta hydrolase -
F6W99_RS21230 4348187..4348789 + 603 WP_003407282.1 lysoplasmalogenase -
F6W99_RS21235 4348870..4350837 + 1968 WP_003407285.1 primosomal protein N' -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(2-108)

Antitoxin

Ribbon-helix-helix protein, CopG

(3-39)


Sequences

Toxin        


Download         Length: 134 a.a.        Molecular weight: 14952.43 Da        Isoelectric Point: 11.4656

>T140788 WP_003898861.1 NZ_CP044345:c4345770-4345369 [Mycobacterium tuberculosis]
MILVDSDVLIAHLRGVVAARDWLVSARKDGPLAISVVSTAELIGGMRTAERREVWRLLASFRVQPATEVIARRAGDMMRR
YRRSHNRIGLGDYLIAATADVQDLQLATLNVWHFPMFEQLKPPFAVPGHRPRA

Download         Length: 402 bp

>T140788 NZ_CP059424:49123-49608 [Enterobacter hormaechei]
GTGGGACGTGTAACAGTACCAGAACCTTTATCCAGCTTTCATCAGGTGGCGGAATTCGTTTGCGGTGAAACTGTTCTGGA
TGACTGGTTAAAGCAGAAAGGCCTCAAAAACCAGGCTCTCGGATCGGCCAGAACGTTTGTAGTGTGTAAAAAAGACACGA
AACAAGTAGCCGGTTTTTACTCTCTGGCCACCGGGAGCGTCAACCATACAGAAGCGACAGGCAATCTTCGGCGTAACATG
CCCGATCCCATCCCTGTTATTATACTTGCCCGTCTTGCTGTCGATCTCTCTTTCCGCGGAAAAGGGCTTGGCGCTGATTT
ACTTCATGATGCAGTGCTTCGTTGCTATCGGGTTGCCGAGAATATTGGTGTACGTGCAATCATGGTTCATGCACTCACCG
AAGAAGCTAAAAATTTCTACATTCACCATGGTTTCAAACCATCACAAACTCAGCAACGGACATTATTTCTTAAACTCCCT
CAATAG

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9443.58 Da        Isoelectric Point: 6.4631

>AT140788 WP_003407272.1 NZ_CP044345:c4346024-4345767 [Mycobacterium tuberculosis]
MKRTNIYLDEEQTASLDKLAAQEGVSRAELIRLLLNRALTTAGDDLASDLQAINDSFGTLRHLDPPVRRSGGREQHLAQV
WRATS

Download         Length: 258 bp

>AT140788 NZ_CP059424:48869-49135 [Enterobacter hormaechei]
ATGAAATCAGATGTTCAACTTAATCTTAGAGCAAAGGAATCACAGCGGGCGCTCATTGATGCTGCTGCGGAAATTCTTCA
CAAATCACGAACAGACTTCATCCTGGAAATGGCCTGTCAGGCCGCTGAGAACGTGATCCTCGATCGCCGGGTCTTTAATT
TTAACGATGAACAATATGCCGAATTCATCGACATGCTTGATGCCCCTGTTGCAGATGATCCTGCCATAGATAAACTGCTG
GCAAGGAAACCTCAGTGGGACGTGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A045ISB0

Antitoxin

Source ID Structure
AlphaFold DB G0TIK2

References