Detailed information of TA system TA140785

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 4010676..4011244
• Replicon: chromosome
• Accession: NZ_CP044345
• Organism: Mycobacterium tuberculosis strain L

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TH08
• Locus tag: F6W99_RS19655
• Protein ID: WP_003405865.1
• Coordinates: 4010870..4011244 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TH07
• Locus tag: F6W99_RS19650
• Protein ID: WP_003405863.1
• Coordinates: 4010676..4010873 (+)
• Length: 66 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F6W99_RS19630	4006717..4007355	-	639	WP_003898730.1	lipid droplet-associated protein	-
F6W99_RS19635	4007445..4008452	+	1008	WP_003405853.1	4-hydroxy-3-methylbut-2-enyl diphosphate reductase	-
F6W99_RS19640	4008469..4009452	-	984	WP_003898731.1	hypothetical protein	-
F6W99_RS19645	4009515..4010588	+	1074	WP_003898732.1	redox-regulated ATPase YchF	-
F6W99_RS19650	4010676..4010873	+	198	WP_003405863.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
F6W99_RS19655	4010870..4011244	+	375	WP_003405865.1	type II toxin-antitoxin system VapC family toxin	Toxin
F6W99_RS19660	4011447..4012145	+	699	WP_003898733.1	hypothetical protein	-
F6W99_RS19665	4012263..4012448	+	186	WP_003901093.1	hypothetical protein	-
F6W99_RS19670	4012375..4012650	-	276	WP_003405867.1	hypothetical protein	-
F6W99_RS19675	4012893..4013216	+	324	WP_003405871.1	antibiotic biosynthesis monooxygenase	-
F6W99_RS19680	4013231..4014091	-	861	WP_003898734.1	hypothetical protein	-
F6W99_RS19685	4014124..4014894	-	771	Protein_3726	adenylate/guanylate cyclase domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-111)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-42)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13444.56 Da
• Isoelectric Point: 5.1881

>T140785 WP_003405865.1 NZ_CP044345:4010870-4011244 [Mycobacterium tuberculosis]
VILVDTSVWIEHLRAADARLVELLGDDEAGCHPLVIEELALGSIKQRDVVLDLLANLYQFPVVTHDEVLRLVGRRRLWGR
GLGAVDANLLGSVALVGGARLWTRDKRLKAACAESGVALAEEVS

Nucleotide

• Length: 375 bp

>T140785 NZ_CP059422:c3954828-3954418 [Enterobacter hormaechei]
GTGGTCCTGTGGCAATCTGATCTTCGCGTCTCGTGGCGCTCACAGTGGATGTCCTTACTGCTCCACGGGCTGGTGGCGGC
ACTGGTGTTATTGGTGCCGTGGCCGCTTAGCTATACCCCTCTGTGGTTGCTATTGCTGTCGTTTGTGGTGTTTGACAGCG
TGCGCAGCCAGCGTCGAATCAACGCCCGTCAGGGTGAAATTAAGCTGCTGATGGATTCTCGCCTGCGCTGGCAGGGAAAA
GAGTGGGAGATGATGGGGACGCCGTGGATGCTTAATACCGGGATGATGCTACGGCTGCGTCGGGTCGAGGATAACCGTCG
CCAGCACCTGTGGCTGGCCGCAGACAGTATGGATCCTGCGGAGTGGCGAGATCTGCGCCGGTTGATCGTGCAACAGCCGA
CGCAGGAGTAA

Antitoxin

Protein

• Length: 66 a.a.
• Molecular weight: 6973.03 Da
• Isoelectric Point: 11.7327

>AT140785 WP_003405863.1 NZ_CP044345:4010676-4010873 [Mycobacterium tuberculosis]
MRTTVTVDDALLAKAAELTGVKEKSTLLREGLQTLVRVESARRLAALGGTDPQATAAPRRRTSPR

Nucleotide

• Length: 198 bp

>AT140785 NZ_CP059422:c3955075-3954809 [Enterobacter hormaechei]
ATGGATATTAACAACAAGGCCCGTATTCACTGGGCGTGCCGTCGCGGCATGCGTGAACTTGATATCTCCATCATGCCTTT
CTTCGAGTATGAGTACGACAGCTTAAGCGATGACGATAAGCGCCTGTTTGTCCGTCTGCTTGAGTCTGACGATCCGGATT
TATTCAACTGGCTGATGAATCACGGCAAGCCCGCCGACACCGAGTTGCAACGGATGGTGCAATTAATTCAAACACGGAAT
CAGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• AlphaFold DB: G0TH08

Antitoxin

• AlphaFold DB: G0TH07