Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 3794605..3795578 Replicon chromosome
Accession NZ_CP044345
Organism Mycobacterium tuberculosis strain L

Toxin (Protein)


Gene name tacT Uniprot ID O05911
Locus tag F6W99_RS18545 Protein ID WP_003404756.1
Coordinates 3795078..3795578 (+) Length 167 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID O05910
Locus tag F6W99_RS18540 Protein ID WP_003898647.1
Coordinates 3794605..3795081 (+) Length 159 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
F6W99_RS18520 3790452..3791723 - 1272 WP_003404747.1 PPE family protein -
F6W99_RS18525 3791738..3792037 - 300 WP_003404748.1 PE family protein -
F6W99_RS18530 3792481..3794262 + 1782 WP_003404749.1 BCCT family transporter -
F6W99_RS18535 3794259..3794516 + 258 WP_003404750.1 hypothetical protein -
F6W99_RS18540 3794605..3795081 + 477 WP_003898647.1 DUF1778 domain-containing protein Antitoxin
F6W99_RS18545 3795078..3795578 + 501 WP_003404756.1 GNAT family N-acetyltransferase Toxin
F6W99_RS18555 3795891..3797210 - 1320 WP_003404757.1 IS256-like element IS1554 family transposase -
F6W99_RS18560 3797498..3798079 + 582 WP_003404760.1 IS607-like element IS1535 family transposase -
F6W99_RS18565 3798079..3799731 + 1653 WP_003404761.1 IS607 family element transposase accessory protein TnpB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 3794259..3846954 52695


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(38-143)

Antitoxin

Vibrio phage ICP1, Orf50

(74-150)


Sequences

Toxin        


Download         Length: 167 a.a.        Molecular weight: 18048.55 Da        Isoelectric Point: 7.8659

>T140782 WP_003404756.1 NZ_CP044345:3795078-3795578 [Mycobacterium tuberculosis]
VSGYSAPRRISDADDVTSFSSGEPSLDDYLRKRALANHVQGGSRCFVTCRDGRVVGFYALASGSVAHADAPGRVRRNMPD
PVPVILLSRLAVDRKEQGRGLGSHLLRDAIGRCVQAADSIGLRAILVHALHDEARAFYVHFDFEISPTDPLHLMLLMKDA
RALIGD

Download         Length: 501 bp

>T140782 NZ_CP059422:c1925043-1924864 [Enterobacter hormaechei]
ATGGATAGCAGGAATGCAATAGCAATGATAGAAGCCGATGGGTGGTATCTAGTGAGAGTTAAAGGCAGTCATCACCAGTT
CAAACACCCAACGAAAAAGGGGCTGGTAACGGTAAAGCATCCACAGAAAGACATACCGTTACCAACACTGAAAAGCATCA
AAAAACAGGCGGGGCTCTAA

Antitoxin


Download         Length: 159 a.a.        Molecular weight: 17387.98 Da        Isoelectric Point: 11.2467

>AT140782 WP_003898647.1 NZ_CP044345:3794605-3795081 [Mycobacterium tuberculosis]
MHRAGAAVTANVWCRAGGIRMAPRPVIPVATQQRLRRQADRQSLGSSGLPALNCTPIRHTIDVMATKPERKTERLAARLT
PEQDALIRRAAEAEGTDLTNFTVTAALAHARDVLADRRLFVLTDAAWTEFLAALDRPVSHKPRLEKLFAARSIFDTEG

Download         Length: 477 bp

>AT140782 NZ_CP059422:c1924842-1924432 [Enterobacter hormaechei]
ATGTTGTACCCTGTTGCTATTGATAAAGGCGATTCATCCTTCGGCGTTCGCGTACCTGATATTCCTGGCTGCTTCTCTGG
CGGTGATGATTATCAGGACGCGATCGAAAGCGTACGTGAAGCGATCGAGGCACACATCGAATTACTGGTTGAAGATGGCG
AGAGCGTTCCGGAAGCAACGACCGTCGAAAACTGGCTATCTGATCCGGAATACACCGGTGCGGTATGGGCCCTGGTTGAC
GTGGATATAACTCGCCTGATGGGAAAAGCGGAAAAAATCAATGTGACGCTTCCCTCATTGCTGATCCGCCGTATCGATCA
GTTTGTGGCCGCGCATCCAGAATACGGCAGTCGGTCTGGCTTCCTGTCTCGTGTCGCGGCAGATAAAGTAATCGGACGAG
AAAAACGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4BSZ2

Antitoxin

Source ID Structure
AlphaFold DB A5U0V8

References