Detailed information of TA system TA140776

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 3523307..3523940
• Replicon: chromosome
• Accession: NZ_CP044345
• Organism: Mycobacterium tuberculosis strain L

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: F6W99_RS17070
• Protein ID: WP_003912919.1
• Coordinates: 3523307..3523690 (-)
• Length: 128 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: P9WJ56
• Locus tag: F6W99_RS17075
• Protein ID: WP_003403368.1
• Coordinates: 3523785..3523940 (-)
• Length: 52 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F6W99_RS17045	3518599..3519135	+	537	WP_003403341.1	50S ribosomal protein L10	-
F6W99_RS17050	3519172..3519564	+	393	WP_003403353.1	50S ribosomal protein L7/L12	-
F6W99_RS17055	3519557..3520252	-	696	WP_003403355.1	TetR/AcrR family transcriptional regulator	-
F6W99_RS17060	3520323..3521828	+	1506	WP_003403360.1	carotenoid cleavage oxygenase	-
F6W99_RS17065	3521909..3522919	+	1011	WP_003911248.1	ABC transporter ATP-binding protein	-
F6W99_RS17070	3523307..3523690	-	384	WP_003912919.1	ribonuclease VapC6	Toxin
F6W99_RS17075	3523785..3523940	-	156	WP_003403368.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
F6W99_RS17080	3524016..3524732	-	717	WP_003403371.1	CPBP family intramembrane metalloprotease	-
F6W99_RS17085	3525008..3525316	-	309	WP_003403376.1	type II toxin-antitoxin system PemK/MazF family toxin	-
F6W99_RS17090	3525303..3525548	-	246	WP_003403381.1	ribbon-helix-helix protein, CopG family	-
F6W99_RS17095	3525658..3526095	-	438	WP_003403386.1	type II toxin-antitoxin system VapC family toxin	-
F6W99_RS17100	3526092..3526346	-	255	WP_003911263.1	antitoxin VapB7	-
F6W99_RS17105	3526460..3528823	+	2364	WP_003898547.1	arylsulfatase AtsD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (3-44)

Sequences

Toxin

Protein

• Length: 128 a.a.
• Molecular weight: 13989.76 Da
• Isoelectric Point: 6.0803

>T140776 WP_003912919.1 NZ_CP044345:c3523690-3523307 [Mycobacterium tuberculosis]
MAAATTTGTHRGLELRAAQRAVGSCEPQRAEFCRSARNADEFDQMSRMFGDVYPDVPVPKSVWRWIDSAQHRLARAGAVG
ALSVVDLLICDTATARGLVVLHDDADYELAERHLPDIRVRRVVSADD

Nucleotide

• Length: 384 bp

>T140776 NZ_CP059421:c208016-207729 [Enterobacter hormaechei]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin

Protein

• Length: 52 a.a.
• Molecular weight: 5872.72 Da
• Isoelectric Point: 5.7734

>AT140776 WP_003403368.1 NZ_CP044345:c3523940-3523785 [Mycobacterium tuberculosis]
VSVTQIDLDDEALADVMRIAAVHTKKEAVNLAMRDYVERFRRIEALARSRE

Nucleotide

• Length: 156 bp

>AT140776 NZ_CP059421:c208255-208016 [Enterobacter hormaechei]
ATGGGTAGCATTAACCTACGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGCGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGACTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Structures

Antitoxin

• AlphaFold DB: A0A7U4BSF8