Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1-PHD
Location 3464629..3465275 Replicon chromosome
Accession NZ_CP044345
Organism Mycobacterium tuberculosis strain L

Toxin (Protein)


Gene name vapC Uniprot ID O07783
Locus tag F6W99_RS16715 Protein ID WP_003403122.1
Coordinates 3464629..3465021 (-) Length 131 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID G0TQ86
Locus tag F6W99_RS16720 Protein ID WP_003403125.1
Coordinates 3465018..3465275 (-) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
F6W99_RS16700 3460291..3461817 + 1527 WP_003403112.1 virulence factor Mce family protein -
F6W99_RS16705 3461814..3463022 + 1209 WP_003403114.1 virulence factor Mce family protein -
F6W99_RS16710 3463027..3464577 + 1551 WP_003403119.1 MCE family protein -
F6W99_RS16715 3464629..3465021 - 393 WP_003403122.1 type II toxin-antitoxin system toxin ribonuclease C4 Toxin
F6W99_RS16720 3465018..3465275 - 258 WP_003403125.1 type II toxin-antitoxin system antitoxin VapB4 Antitoxin
F6W99_RS16725 3465458..3466693 - 1236 WP_003403128.1 ATP-binding protein -
F6W99_RS16730 3466944..3467357 - 414 WP_003403137.1 type II toxin-antitoxin system VapC family toxin -
F6W99_RS16735 3467354..3467590 - 237 WP_003403139.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
F6W99_RS16740 3467694..3468200 - 507 WP_003900973.1 two component system sensor kinase HK1 -
F6W99_RS16745 3468314..3468784 - 471 WP_003403144.1 HAMP domain-containing protein -
F6W99_RS16750 3468828..3469589 - 762 WP_003403148.1 two-component system response regulator TcrA -
F6W99_RS16755 3469646..3469957 + 312 WP_003403164.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(8-122)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(3-53)


Sequences

Toxin        


Download         Length: 131 a.a.        Molecular weight: 14080.08 Da        Isoelectric Point: 4.6558

>T140770 WP_003403122.1 NZ_CP044345:c3465021-3464629 [Mycobacterium tuberculosis]
VNVRRALADTSVFIGIEATRFDPDRFAGYEWGVSVVTLGELRLGVLQASGPEAAARRLSTYQLAQRFEPLGIDEAVSEAW
ALLVSKLRAAKLRVPINDSWIAATAVAHGIAILTQDNDYAAMPDVEVITI

Download         Length: 393 bp

>T140770 NZ_CP059419:c1283156-1282938 [Enterobacter hormaechei]
ATGTCTGATAAACCATTAACTAAAGTCGATTATTTGATGCGCCTGCGACGCTGTCAGTCAATTGACACGCTCGAACGAGT
TATTGAGAAAAATAAATACGAGCTGTCTGATAATGAACTGGCGGTATTTTATTCAGCTGCCGACCATCGTCTTGCTGAAC
TGACTATGAATAAACTCTATGACAAGATCCCCTCTTCGGTATGGAAATTTGTTCGTTAG

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9681.06 Da        Isoelectric Point: 7.0175

>AT140770 WP_003403125.1 NZ_CP044345:c3465275-3465018 [Mycobacterium tuberculosis]
MSATIPARDLRNHTAEVLRRVAAGEEIEVLKDNRPVARIVPLKRRRQWLPAAEVIGELVRLGPDTTNLGEELRETLTQTT
DDVRW

Download         Length: 258 bp

>AT140770 NZ_CP059419:c1283559-1283185 [Enterobacter hormaechei]
ATGGACGAGTACTCGCCAAAAAGGCATGATATCGCGCAGTTGAAATTTCTCTGCGAATCCTTGTACCATGACTGCCTTGC
CAATCTTGAAGAAAGTAACCATGGCTGGGTGAACGATCCAACGTCTGCTATCAATTTACAGCTTAATGAGCTGATCGAGC
ATATCGCAACCTTCGCACTTAATTATAAAATTAAGTACAATGAAGATAATAAGTTGATAGAGCAAATTGATGAATACCTG
GACGACACCTTTATGTTGTTCAGCAGCTACGGCATTAACGCACAGGATTTGCAGAAATGGCGTAAATCAGGAAACCGTCT
ATTTCGCTGCTTCACGAATGTCAGCAGAGCTAATCCGGTTAGTCTTTCCTGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4F8V4

Antitoxin

Source ID Structure
AlphaFold DB G0TQ86

References