Detailed information of TA system TA140768

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN-CcdA
• Location: 3410019..3410695
• Replicon: chromosome
• Accession: NZ_CP044345
• Organism: Mycobacterium tuberculosis strain L

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TPQ9
• Locus tag: F6W99_RS16465
• Protein ID: WP_003898500.1
• Coordinates: 3410019..3410432 (-)
• Length: 138 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TPR0
• Locus tag: F6W99_RS16470
• Protein ID: WP_003402915.1
• Coordinates: 3410429..3410695 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F6W99_RS16435	3405364..3405666	-	303	WP_003402896.1	DUF3349 domain-containing protein	-
F6W99_RS16440	3405726..3406004	-	279	WP_003402901.1	hypothetical protein	-
F6W99_RS16445	3406001..3407254	-	1254	WP_003402904.1	inorganic phosphate transporter family protein	-
F6W99_RS16450	3407374..3407760	-	387	WP_003402907.1	VOC family protein	-
F6W99_RS16455	3407823..3408707	-	885	WP_003402909.1	SDR family oxidoreductase	-
F6W99_RS16460	3408803..3409705	-	903	WP_003915439.1	1,4-dihydroxy-2-naphthoyl-CoA synthase	-
F6W99_RS16465	3410019..3410432	-	414	WP_003898500.1	PIN domain-containing protein	Toxin
F6W99_RS16470	3410429..3410695	-	267	WP_003402915.1	antitoxin VapB3	Antitoxin
F6W99_RS16475	3410887..3412602	-	1716	WP_003402917.1	fatty-acid--CoA ligase FadD8	-
F6W99_RS16480	3412680..3414284	+	1605	WP_003402920.1	amidohydrolase	-
F6W99_RS16485	3414281..3415261	+	981	WP_003402923.1	o-succinylbenzoate synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Pae0151-like, PIN domain (13-129)

Antitoxin

• Post-segregation antitoxin CcdA (19-61)

Sequences

Toxin

Protein

• Length: 138 a.a.
• Molecular weight: 14667.78 Da
• Isoelectric Point: 7.3603

>T140768 WP_003898500.1 NZ_CP044345:c3410432-3410019 [Mycobacterium tuberculosis]
VRASPTSPPEQVVVDASAMVDLLARTSDRCSAVRARLARTAMHAPAHFDAEVLSALGRMQRAGALTVAYVDAALEELRQV
PVTRHGLSSLLAGAWSRRDTLRLTDALYVELAETAGLVLLTTDERLARAWPSAHAIG

Nucleotide

• Length: 414 bp

>T140768 NZ_CP059418:251686-252171 [Enterobacter hormaechei]
GTGGGACGTGTAACAGCACCAGAACCATTATCCAGCTCCCATCAACTAGCAGAGTTCGTCAGTGGTGAGGCAGTACTTGA
TGACTGGCTTAAACAGAGGGGATTAAAAAACCAAGCTCTTGGTGCAGCACGAACGTTCGTCGTTTGTAAAAGAGACACTA
AACAGGTTGTCGGTTTTTACTCTCTGGCCACCGGCAGCGTTAACCACGTGGAAGCAATGGGAAGCCTCAGGCGCAATATG
CCAGATCCAATTCCCGTAATCATACTTGCTCGCCTGGCAGTTGATGTTTCATACCGTGGCCAAGGGCTCGGGGCCGATTT
ACTGCATGATGCAGTTCTGCGCTGTTACCGTGTAGCTGAGAATATAGGAGTCCGTGCGATTATGGTTCATGCACTGACTG
AAGAAGCCAAAAACTTTTATCTTCACCATGGCTTTAAGGCATCACAAACTCAGGAACGGACATTGTTTTTAAAGTTACCA
CAGTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9543.79 Da
• Isoelectric Point: 4.5994

>AT140768 WP_003402915.1 NZ_CP044345:c3410695-3410429 [Mycobacterium tuberculosis]
MLSRRTKTIVVCTLVCMARLNVYVPDELAERARARGLNVSALTQAAISAELENSATDAWLEGLEPRSTGARHDDVLGAID
AARDEFEA

Nucleotide

• Length: 267 bp

>AT140768 NZ_CP059418:251432-251698 [Enterobacter hormaechei]
ATGAAATCAGATGTTCAGCTCAACCTTAGAGCTAAAGAATCTCAAAGAGCACTTATTGATGCTGCAGCAGAGATTCTCCA
TAAATCACGCACGGACTTCATCCTGGAAATGGCATGTCAGGCTGCGGAGAATGTGATCCTCGATCGTCGCGTATTTAATT
TTAACGATGAGCAGTATGCAGAGTTTATCGATATGCTCGATGCACCGGTTTCGGATGAGGCGGCTATTGATAAACTTCTG
GCAAGGAAACCTCAGTGGGACGTGTAA

Structures

Toxin

• AlphaFold DB: G0TPQ9

Antitoxin

• AlphaFold DB: G0TPR0